连城白鸭不同组织中主要矿物元素沉积规律与关键基因表达水平研究

doi:10.3969/j.issn.1004-1524.2021.12.06

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (12): 2264-2274.DOI: 10.3969/j.issn.1004-1524.2021.12.06

连城白鸭不同组织中主要矿物元素沉积规律与关键基因表达水平研究

白皓¹(), 李潇凡², 仲黎², 宋倩倩², 江勇², 张扬², 王志秀², 徐琪², 常国斌¹^,², 陈国宏¹^,²^,^*()

1.扬州大学农业科技发展研究院,教育部农业与农产品安全国际合作联合实验室,江苏扬州 225009
2.扬州大学动物科学与技术学院,江苏扬州 225009

收稿日期:2020-08-25 出版日期:2021-12-25 发布日期:2022-01-10
通讯作者: 陈国宏
作者简介:* 陈国宏,E-mail: ghchen@yzu.edu.cn
白皓(1987—),男,江苏扬州人,博士,助理研究员,主要从事家禽遗传育种研究。E-mail: bhowen1027@yzu.edu.cn
基金资助:
国家现代农业产业技术体系(CARS-42-3);江苏现代农业(水禽)产业技术体系(JATS[2019]310);江苏省农业科技自主创新资金(CX181004)

Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

BAI Hao¹(), LI Xiaofan², ZHONG Li², SONG Qianqian², JIANG Yong², ZHANG Yang², WANG Zhixiu², XU Qi², CHANG Guobin¹^,², CHEN Guohong¹^,²^,^*()

1. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China
2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2020-08-25 Online:2021-12-25 Published:2022-01-10
Contact: CHEN Guohong

摘要/Abstract

摘要：

为了探讨连城白鸭不同组织中主要矿物元素的沉积规律及相关基因mRNA的表达水平,本研究利用原子荧光光度计,分别测定了0、21、35、49和63日龄连城白鸭胸肌、腿肌、肝脏、背皮和胫骨组织中的镁、钾、铁、锌和硒等矿物元素含量,采用qRT-PCR法分析不同日龄肝脏组织中各矿物元素相关基因表达水平及其与矿物元素沉积之间的关系。结果表明:镁与钾元素的沉积规律在所有组织中基本一致,且两者呈显著性正相关(P<0.05),在胸肌、腿肌和肝脏组织中,几乎所有矿物元素的含量在49~63日龄达到峰值(P<0.05)。镁元素相关基因TRPM6和TRPM7在21日龄时的表达水平最高(P<0.05);钾元素相关基因ATP1A1和ATP1B1在0日龄时的表达水平最高(P<0.05);铁元素相关基因FTH1的表达规律表现为先显著性下降再显著性升高的趋势,在35日龄时的表达水平最低(P<0.05);锌元素相关基因ATP6和ATP8分别在0和21日龄时的表达水平最高(P<0.05);硒元素相关基因GPX1和GPX4均在0日龄时的表达水平最高(P<0.05)。此外所有元素相关基因的表达水平与元素在肝脏组织中的沉积量呈负相关,但不显著(P>0.05)。综上,镁元素和钾元素可作为联合指标用于连城白鸭优质品系的精准选育;49~63日龄可作为连城白鸭最佳出栏日龄的参考日龄。

关键词: 连城白鸭, 矿物元素, 基因表达, 相关性

Abstract:

This study was conducted to investigate the depositions of several mineral elements and the mRNA expression levels of mineral-related genes in different tissues of Liancheng white duck. The contents of Mg, K, Fe, Zn and Se in breast muscle, thigh muscle, liver, skin and tibia at 0, 21, 35, 49 and 63 days of age, respectively, were measured by atomic fluorescence spectrophotometer. The mRNA expression levels of mineral-related genes were detected by qRT-PCR. The results revealed that the depositions of Mg and K were basically the same in all tissues, showing a significant positive correlation (P<0.05). In view of the breast muscle, thigh muscle and liver, the contents of almost all the mineral elements reached the peak value (P<0.05) at the age of 49-63 days. The expression levels of Mg-related genes TRPM6 and TRPM7 were the highest at the age of 21 days (P<0.05); the expression levels of K-related genes ATP1A1 and ATP1B1 were the highest at birth (P<0.05); the expression of Fe-related gene FTH1 showed a significant decrease and then a significant increase with the change of age, which had the lowest expression at the age of 35 days (P<0.05); the expression levels of Zn-related genes ATP6 and ATP8 were the highest at 0 and 21 days, respectively (P<0.05); the expression levels of Se-related genes GPX1 and GPX4 were the highest at birth (P<0.05). In addition, there were negative correlations between the expression level of genes and the deposition of mineral elements in liver tissue but not significantly (P>0.05). Taken together, Mg and K could be used as joint indicators for the precise breeding of high quality strain of Liancheng white duck; 49-63 days can be used as the reference of the best marketing age.

Key words: Liancheng white duck, mineral elements, gene expression, correlation

中图分类号:

S834

白皓, 李潇凡, 仲黎, 宋倩倩, 江勇, 张扬, 王志秀, 徐琪, 常国斌, 陈国宏. 连城白鸭不同组织中主要矿物元素沉积规律与关键基因表达水平研究[J]. 浙江农业学报, 2021, 33(12): 2264-2274.

BAI Hao, LI Xiaofan, ZHONG Li, SONG Qianqian, JIANG Yong, ZHANG Yang, WANG Zhixiu, XU Qi, CHANG Guobin, CHEN Guohong. Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2264-2274.

图/表 10

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项目Item	0~7 d	8~21 d	22~42 d	43~63 d
原料Ingredient
玉米Corn/%	10.32	10.63	47.18	21.27
次粉Wheat middling/%	15.41	15.00	6.89	20.00
麦麸Wheat bran/%	-	-	20.00	30.01
米粉Rice noodles/%	35.21	34.99	-	10.00
米糠Rice bran/%	15.81	15.00	3.00	5.00
花生粉Peanut meal/%	-	-	3.00	2.37
玉米蛋白粉Corn gluten meal/%	-	-	5.00	-
豆粕Soybean meal/%	12.63	13.70	5.94	2.50
核苷酸渣Nucleotide slag/%	2.00	2.00	-	-
石灰石粉Limestone powder/%	1.52	1.58	1.90	1.96
磷酸氢钙Calcium hydrogen phosphate/%	1.10	1.10	1.01	0.84
复合预混料Compound premix^a/%	6.00	6.00	6.00	6.00
配方营养成分Formulated nutrient profile
粗蛋白Crude protein/(g·kg^-1)	185.00	190.00	170.00	170.00
粗脂肪Crude fat/(g·kg^-1)	20.00	30.00	35.00	35.00
粗纤维Crude fiber/(g·kg^-1)	60.00	50.00	70.00	70.00
粗灰分Crude ash/(g·kg^-1)	90.00	80.00	100.00	100.00
钙Calcium/(g·kg^-1)	10.00	10.00	10.00	10.00
磷Phosphorus/(g·kg^-1)	5.00	5.50	4.50	4.50
氯化钠Sodium chloride/(g·kg^-1)	6.00	6.00	6.00	6.00
蛋氨酸Methionine/(g·kg^-1)	4.00	4.00	2.80	2.80
水分Moisture/(g·kg^-1)	140.00	130.00	130.00	130.00

项目Item	0~7 d	8~21 d	22~42 d	43~63 d
原料Ingredient
玉米Corn/%	10.32	10.63	47.18	21.27
次粉Wheat middling/%	15.41	15.00	6.89	20.00
麦麸Wheat bran/%	-	-	20.00	30.01
米粉Rice noodles/%	35.21	34.99	-	10.00
米糠Rice bran/%	15.81	15.00	3.00	5.00
花生粉Peanut meal/%	-	-	3.00	2.37
玉米蛋白粉Corn gluten meal/%	-	-	5.00	-
豆粕Soybean meal/%	12.63	13.70	5.94	2.50
核苷酸渣Nucleotide slag/%	2.00	2.00	-	-
石灰石粉Limestone powder/%	1.52	1.58	1.90	1.96
磷酸氢钙Calcium hydrogen phosphate/%	1.10	1.10	1.01	0.84
复合预混料Compound premix^a/%	6.00	6.00	6.00	6.00
配方营养成分Formulated nutrient profile
粗蛋白Crude protein/(g·kg^-1)	185.00	190.00	170.00	170.00
粗脂肪Crude fat/(g·kg^-1)	20.00	30.00	35.00	35.00
粗纤维Crude fiber/(g·kg^-1)	60.00	50.00	70.00	70.00
粗灰分Crude ash/(g·kg^-1)	90.00	80.00	100.00	100.00
钙Calcium/(g·kg^-1)	10.00	10.00	10.00	10.00
磷Phosphorus/(g·kg^-1)	5.00	5.50	4.50	4.50
氯化钠Sodium chloride/(g·kg^-1)	6.00	6.00	6.00	6.00
蛋氨酸Methionine/(g·kg^-1)	4.00	4.00	2.80	2.80
水分Moisture/(g·kg^-1)	140.00	130.00	130.00	130.00

基因 Gene	引物序列(5'-3') Sequence of primer (5'-3')	产物长度 Product length/bp	退火温度 Tm/℃
TRPM6	F:TTGCAAGGTGTTGGGGAAAAC	199	60
	R:GCCTTTCCATTGTGCAGTCG
TRPM7	F:TGATTGATGTGGGGCTGGTT	124	60
	R:GCACCAGTGTCAATCCGACT
ATP1A1	F:TGAGCCTACTGCAACATCCG	168	60
	R:GCAGTAGTCAAACCCCGACT
ATP1B1	F:TGTGCTCCCAAGAGAGACGA	112	60
	R:GAAGCTTGCCGTAGTAGGGG
FTH1	F:AATGGGCTGACTGCAATGGA	109	60
	R:CACACAGGTGTGGGTCGTT
GPX1	F:ACTTCCTGCAGCTCAACGAG	103	60
	R:TTGGTGGCATTCTCCTGGTG
GPX4	F:GACAACGCGCAGATTAAGGC	152	60
	R:TTTATGGCATTGCCCAGGGT
ATP6	F:TTGGCATCCCCCTGATCCTA	167	60
	R:GGCTCATTTGTGGCCGTTTT
ATP8	F:CCTGACTAACCCTCGCACTC	114	60
	R:ATGGTCAGGCTCATGGTGTG
β-actin	F:GTGCTATGTCGCCCTGGATT	171	60
	R:CCACAGGACTCCATACCCAAG

基因 Gene	引物序列(5'-3') Sequence of primer (5'-3')	产物长度 Product length/bp	退火温度 Tm/℃
TRPM6	F:TTGCAAGGTGTTGGGGAAAAC	199	60
	R:GCCTTTCCATTGTGCAGTCG
TRPM7	F:TGATTGATGTGGGGCTGGTT	124	60
	R:GCACCAGTGTCAATCCGACT
ATP1A1	F:TGAGCCTACTGCAACATCCG	168	60
	R:GCAGTAGTCAAACCCCGACT
ATP1B1	F:TGTGCTCCCAAGAGAGACGA	112	60
	R:GAAGCTTGCCGTAGTAGGGG
FTH1	F:AATGGGCTGACTGCAATGGA	109	60
	R:CACACAGGTGTGGGTCGTT
GPX1	F:ACTTCCTGCAGCTCAACGAG	103	60
	R:TTGGTGGCATTCTCCTGGTG
GPX4	F:GACAACGCGCAGATTAAGGC	152	60
	R:TTTATGGCATTGCCCAGGGT
ATP6	F:TTGGCATCCCCCTGATCCTA	167	60
	R:GGCTCATTTGTGGCCGTTTT
ATP8	F:CCTGACTAACCCTCGCACTC	114	60
	R:ATGGTCAGGCTCATGGTGTG
β-actin	F:GTGCTATGTCGCCCTGGATT	171	60
	R:CCACAGGACTCCATACCCAAG

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.88^**	1
Fe	0.43	0.48	1
Se	-0.57	-0.54	0.01	1
Zn	0.22	0.21	0.22	0.62	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.67^*	1
Fe	0.19	0.01	1
Se	-0.10	0.44	0.08	1
Zn	-0.37	-0.30	0.05	0.33	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.22	1
Fe	0.01	-0.30	1
Se	0.08	-0.30	0.49	1
Zn	-0.13	-0.30	0.27	-0.10	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.84^**	1
Fe	0.68	0.49	1
Se	0.46	0.14	0.66	1
Zn	-0.13	-0.20	0.52	0.04	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.23	1
Fe	-0.51	-0.06	1
Se	-0.43	0.02	0.71^*	1
Zn	0.59	0.08	-0.35	0.10	1

连城白鸭不同组织中主要矿物元素沉积规律与关键基因表达水平研究

Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

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0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.69^*	1
Fe	-0.11	0.11	1
Se	0.12	-0.10	0.42	1
Zn	0.29	0.52	0.78^*	0.44	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.68^*	1
Fe	0.21	0.44	1
Se	0.27	0.20	-0.08	1
Zn	0.02	0.33	0.75^*	0.22	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.79^**	1
Fe	0.35	0.07	1
Se	-0.04	0.03	0.29	1
Zn	0.29	0.45	0.35	0.01	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.21	1
Fe	-0.50	0.62	1
Se	-0.07	0.20	0.52	1
Zn	-0.61	0.01	0.86^*	0.19	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.79^**	1
Fe	0.27	0.56	1
Se	-0.41	-0.17	-0.20	1
Zn	-0.06	0.14	0.63	0.20	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.92^**	1
Fe	-0.10	-0.22	1
Se	0.76^*	0.64^*	-0.30	1
Zn	0.15	0.09	0.61	0.01	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.64^*	1
Fe	0.46	0.43	1
Se	0.95^**	0.32	0.42	1
Zn	0.74^*	0.49	0.79^*	0.70^*	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.36	1
Fe	0.16	0.10	1
Se	0.55	0.38	0.79^*	1
Zn	0.74^*	0.71^*	0.18	0.59	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.71^*	1
Fe	0.19	0.33	1
Se	0.65^*	0.78^**	0.57	1
Zn	0.63	0.61	0.24	0.54	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.76^*	1
Fe	-0.54	0.09	1
Se	0.10	0.55	0.07	1
Zn	0.13	-0.50	-0.50	0.50	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.31	1
Fe	0.58	0.38	1
Se	0.45	-0.10	-0.20	1
Zn	-0.50	0.21	-0.10	-0.60	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.99^**	1
Fe	0.53	0.49	1
Se	0.86^**	0.86^**	0.18	1
Zn	0.98^**	0.97^**	0.51	0.88^**	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.93^**	1
Fe	0.43	-0.50	1
Se	0.05	0.38	-0.10	1
Zn	0.77^*	-0.10	0.72^*	-0.30	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.98^**	1
Fe	0.02	-0.10	1
Se	0.53	0.52	-0.30	1
Zn	-0.1	0.01	-0.60	0.21	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.85^**	1
Fe	0.56	-0.10	1
Se	-0.50	-0.40	-0.20	1
Zn	0.46	0.04	0.45	0.40	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.90^*	1
Fe	0.96	-0.80	1
Se	-0.90	0.25	-0.90	1
Zn	0.67	-0.70	0.10	0.21	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.93^*	1
Fe	-0.72	-0.90	1
Se	-0.30	-0.10	0.65	1
Zn	0.14	-0.20	-0.80	-0.10	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.47	1
Fe	0.08	-0.10	1
Se	0.74	-0.80^*	-0.16	1
Zn	0.36	0.53	0.50	-0.30	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.36	1
Fe	0.94	0.35	1
Se	-0.50	-0.40	-0.10	1
Zn	-0.90	0.26	-0.70	0.17	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.72^*	1
Fe	0.28	0.85	1
Se	0.72	-0.70	-0.50	1
Zn	-0.90^*	0.6	0.31	-0.80^*	1

基因 Gene	矿物元素Mineral elements
基因 Gene	Mg	K	Fe	Se	Zn
TRPM6	-0.482	—	—	—	—
TRPM7	-0.601	—	—	—	—
ATP1A1	—	-0.821	—	—	—
ATP1B1	—	-0.770	—	—	—
FTH1	—	—	-0.651	—	—
GPX1	—	—	—	-0.001	—
GPX4	—	—	—	-0.729	—
ATP6	—	—	—		-0.798
ATP8	—	—	—	—	-0.730

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