根系修剪对枳生长及相关基因表达的影响

doi:10.3969/j.issn.1004-1524.2021.02.10

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (2): 270-277.DOI: 10.3969/j.issn.1004-1524.2021.02.10

根系修剪对枳生长及相关基因表达的影响

黄咏明(), 宋放, 王策, 姚京磊, 王志静, 何利刚, 吴黎明, 蒋迎春^*()

湖北省农业科学院果树茶叶研究所,湖北武汉 430064

收稿日期:2020-05-18 出版日期:2021-02-25 发布日期:2021-02-25
通讯作者: 蒋迎春
作者简介:*蒋迎春,E-mail: 546447505@qq.com
黄咏明(1990—),男,湖北大冶人,助理研究员,主要从事柑橘菌根生理生态功能研究。E-mail: hym900112@163.com
基金资助:
国家重点研发计划(2019YFD1001400);湖北省农业科学院青年科学基金(2018NKYJJ14);湖北省农业科技创新中心创新团队项目(2019-620-000-001-023);湖北省特色水果创新项目

Effects of root pruning on growth and expression of related genes in Poncirus trifoliata

HUANG Yongming(), SONG Fang, WANG Ce, YAO Jinglei, WANG Zhijing, HE Ligang, WU Liming, JIANG Yingchun^*()

Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430064, China

Received:2020-05-18 Online:2021-02-25 Published:2021-02-25
Contact: JIANG Yingchun

摘要/Abstract

摘要：

以枳实生苗为试验材料,通过两年重复试验分析了不切除主根、切除1/3主根、切除1/2主根、切除2/3主根等4个不同水平根系修剪处理下枳实生苗生长发育、根系构型和营养吸收的情况,并检测了不同水平根系修剪处理对侧根发育关键基因表达量的影响。结果表明,切除1/2主根处理增加了枳实生苗的株高、茎粗、地上部鲜重和根系鲜重,改善了根系构型参数(侧根数、总根长、根表面积和平均长度),增加了根系P、K、Ca、B、Fe、Na和Zn等营养元素含量。另外,实时荧光定量PCR(qRT-PCR)检测发现,根系修剪处理诱导了侧根发育关键基因HOX1和CYP2的积累,并抑制了IAA11和IAA13的表达。综上所述,适度根系修剪可能通过诱导HOX1、CYP2基因的上调和IAA11、IAA13基因的下调来调控枳实生苗侧根的生长发育,改善根系构型,从而提高枳实生苗对营养元素的吸收能力及植株的生长发育。

关键词: 根系修剪, 枳, 根系构型, 基因表达

Abstract:

In this study, Poncirus trifoliata seedlings were chosen as the test materials. The effects of four different root pruning levels (no cutting, cutting off 1/3 taproot, cutting off 1/2 taproot and cutting off 2/3 taproot) on the plant growth, root architecture system and nutrient contents of Poncirus trifoliata seedlings were investigated by a repeated experiment for two years, and the expression of key genes involved in the lateral roots development was analyzed under root pruning treatments. The results showed that cutting off 1/2 taproot treatment enhanced the height, stem diameter, shoot and root fresh weights, and improved the root system architecture parameters (the number of lateral roots, the total root length, the root surface area and average length), and also increased the contents of K, P, Ca, B, Fe, Na and Zn in roots of Poncirus trifoliata seedlings. In addition, quantitative real-time PCR (qRT-PCR) revealed that the expression levels of lateral root development related genes HOX1 and CYP2 were induced, but IAA11 and IAA13 were reduced under root pruning treatments. Overall, the moderate root pruning might regulate the development of lateral roots and improve the root architecture through up-regulating of HOX1 and CYP2 genes, and down-regulating of IAA11 and IAA13 genes, thus leading to the improvement of nutrient absorption and growth of Poncirus trifoliata seedlings.

Key words: root pruning, Poncirus trifoliata, root system architecture, gene expression

中图分类号:

S666.9

黄咏明, 宋放, 王策, 姚京磊, 王志静, 何利刚, 吴黎明, 蒋迎春. 根系修剪对枳生长及相关基因表达的影响[J]. 浙江农业学报, 2021, 33(2): 270-277.

HUANG Yongming, SONG Fang, WANG Ce, YAO Jinglei, WANG Zhijing, HE Ligang, WU Liming, JIANG Yingchun. Effects of root pruning on growth and expression of related genes in Poncirus trifoliata[J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 270-277.

图/表 6

参考文献 36

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引物名称 Primer name	引物序列 Primer sequences(5'-3')
IAA11-F	TGTTCCACCAGCCAACAACT
IAA11-R	AGCCAAGAAACATGCCCCAT
IAA13-F	ACTGGGCTTGAGTCTTGGTG
IAA13-R	ACTAGGTCTCCTCCCAGCAG
CYP2-F	GTTCTTCGTCTGTACGGCCA
CYP2-R	GAGCTGGACCCCACTTTCTC
HOX1-F	AAGCCCAAGAAGCGGTATCC
HOX1-R	ATCAACGGTTGGCTCCCATT
eIF1α-F	CCCCAAAGAATCAGAAACCAT
eIF1α-R	TGCCGAGCATACGAAGGAC

引物名称 Primer name	引物序列 Primer sequences(5'-3')
IAA11-F	TGTTCCACCAGCCAACAACT
IAA11-R	AGCCAAGAAACATGCCCCAT
IAA13-F	ACTGGGCTTGAGTCTTGGTG
IAA13-R	ACTAGGTCTCCTCCCAGCAG
CYP2-F	GTTCTTCGTCTGTACGGCCA
CYP2-R	GAGCTGGACCCCACTTTCTC
HOX1-F	AAGCCCAAGAAGCGGTATCC
HOX1-R	ATCAACGGTTGGCTCCCATT
eIF1α-F	CCCCAAAGAATCAGAAACCAT
eIF1α-R	TGCCGAGCATACGAAGGAC

检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	鲜重Fresh weight/g
检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	地上部Shoot	根系Root
2018	RP₀	17.01±2.25 ab	0.28±0.03 ab	0.64±0.03 b	1.27±0.24 b
	RP₁	18.38±1.88 a	0.26±0.01 b	0.65±0.62 ab	1.11±0.91 ab
	RP₂	18.37±1.36 a	0.31±0.04 a	0.72±0.20 a	1.44±0.17 a
	RP₃	15.04±1.63 b	0.28±0.06 ab	0.48±0.09 b	0.84±0.10 c
2019	RP₀	16.15±2.15 bc	0.27±0.05 b	0.59±0.18 b	1.05±0.12 b
	RP₁	18.07±1.86 a	0.25±0.01 c	0.59±0.15 b	0.94±0.23 bc
	RP₂	18.23±1.45 a	0.31±0.04 a	0.65±0.18 a	1.15±0.13 a
	RP₃	15.48±1.80 c	0.28±0.02 ab	0.54±0.11 b	0.86±0.24 c

检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	鲜重Fresh weight/g
检测年份 Detection year	根系修剪处理 Root prunning treatments	株高 Plant height/cm	茎粗 Stem diameter/cm	地上部Shoot	根系Root
2018	RP₀	17.01±2.25 ab	0.28±0.03 ab	0.64±0.03 b	1.27±0.24 b
	RP₁	18.38±1.88 a	0.26±0.01 b	0.65±0.62 ab	1.11±0.91 ab
	RP₂	18.37±1.36 a	0.31±0.04 a	0.72±0.20 a	1.44±0.17 a
	RP₃	15.04±1.63 b	0.28±0.06 ab	0.48±0.09 b	0.84±0.10 c
2019	RP₀	16.15±2.15 bc	0.27±0.05 b	0.59±0.18 b	1.05±0.12 b
	RP₁	18.07±1.86 a	0.25±0.01 c	0.59±0.15 b	0.94±0.23 bc
	RP₂	18.23±1.45 a	0.31±0.04 a	0.65±0.18 a	1.15±0.13 a
	RP₃	15.48±1.80 c	0.28±0.02 ab	0.54±0.11 b	0.86±0.24 c

检测年份 Detection year	根系修剪处理 Root prunning treatments	每株侧根数 Lateral root number per plant	根系总长度 Total root length/cm	表面积 Surface area/cm²	平均长度 Average length/cm
2018	RP₀	148±18 b	254.37±49.02 b	21.35±3.08 b	1.14±0.19 a
	RP₁	164±21 b	235.59±38.41 b	20.06±2.08 b	1.21±0.0.9 a
	RP₂	250±19 a	355.54±28.34 a	33.86±3.53 a	1.31±0.08 a
	RP₃	181±23 b	202.63±16.26 b	16.83±1.39 b	1.06±0.13 a
2019	RP₀	135±11 b	255.20±6.27 b	20.67±0.69 b	1.15±0.12 b
	RP₁	155±10 b	230.02±11.16 b	20.05±0.41 b	1.18±0.10 b
	RP₂	237±23 a	368.73±14.86 a	35.43±2.16 a	1.32±0.06 a
	RP₃	179±16 ab	215.65±17.05 b	18.75±2.08 b	1.03±0.08 b

根系修剪对枳生长及相关基因表达的影响

Effects of root pruning on growth and expression of related genes in Poncirus trifoliata

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检测年份 Detection year	根系修剪处理 Root pruning treatments	大量元素含量 Macro-element content/%			中量元素含量 Secondary element content/%		微量元素含量 Trace-element content/(mg·kg^-1)
检测年份 Detection year	根系修剪处理 Root pruning treatments	N	P	K	Ca	Mg	B	Fe	Na	Zn
2018	RP₀	1.72 ±0.51 c	0.50 ±0.07 b	1.46 ±0.27 c	3.96 ±0.37 c	1.25 ±0.04 c	25.37 ±3.96 c	82.68 ±3.92 c	73.51 ±21.81 c	8.39 ±4.17 c
	RP₁	2.16 ±0.26 ab	0.59 ±0.07 b	2.08 ±0.87 b	4.60 ±1.00 bc	1.42 ±0.18 bc	44.61 ±4.34 a	89.06 ±4.99 bc	90.38 ±13.75 b	10.19 ±7.00 bc
	RP₂	1.99 ±0.05 b	0.78 ±0.11 a	2.67 ±1.08 ab	7.17 ±0.34 a	1.61 ±0.21 b	41.57 ±1.92 ab	125.17 ±5.18 a	108.25 ±7.76 a	19.85 ±3.64 ab
	RP₃	2.26 ±0.15 a	0.72 ±0.04 ab	3.68 ±0.28 a	5.95 ±1.24 ab	1.94 ±0.05 a	26.85 ±1.96 bc	114.41 ±6.43 ab	85.91 ±4.99 bc	25.19 ±2.02 a
2019	RP₀	1.91 ±0.02 b	0.60 ±0.04 c	2.73 ±1.22 b	5.49 ±1.24 c	1.52 ±0.21 b	28.11 ±6.44 b	80.50 ±29.82 b	53.24 ±8.70 b	11.88 ±6.67 c
	RP₁	2.23 ±0.02 ab	0.82 ±0.05 a	3.07 ±0.19 ab	6.93 ±1.08 ab	1.68 ±0.37 ab	34.36 ±1.11 ab	87.34 ±15.41 b	64.35 ±15.65 b	14.85 ±8.46 bc
	RP₂	2.16 ±0.19 b	0.76 ±0.02 ab	3.11 ±0.67 a	7.09 ±0.44 a	1.70 ±0.10 ab	40.38 ±2.93 a	147.03 ±24.8 a	90.37 ±13.75 a	27.01 ±3.75 a
	RP₃	2.32 ±0.03 a	0.74 ±0.05 b	3.17 ±0.71 a	6.46 ±1.10 bc	1.80 ±0.18 a	28.72 ±3.66 b	98.57 ±12.81 b	79.38 ±3.51 b	25.18 ±2.02 ab

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