炭疽病对茶树叶片光合特性的影响

doi:10.3969/j.issn.1004-1524.2020.11.12

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (11): 2020-2026.DOI: 10.3969/j.issn.1004-1524.2020.11.12

炭疽病对茶树叶片光合特性的影响

张政^1,2, 王晓荣³, 钱虹⁴, 张兰¹, 颜鹏¹, 张丽平¹, 张新富², 李鑫^1,*, 韩文炎¹

1.中国农业科学院茶叶研究所,浙江杭州 310008;
2.青岛农业大学园艺学院,山东青岛 266109;
3.河北建筑工程学院经济管理学院,河北张家口 075000;
4.德清县农业技术推广中心,浙江德清 313200

收稿日期:2020-03-03 出版日期:2020-11-25 发布日期:2020-12-02
通讯作者: * 李鑫,E-mail: lixin@tricaas.com
作者简介:张政(1998—),男,山东高密人,硕士研究生,主要从事茶树栽培生理研究。E-mail: zhangzheng3373@163.com
基金资助:
浙江省自然科学基金(Y19C160031); 国家重点研发计划(2017YFE0107500)

Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.)

ZHANG Zheng^1,2, WANG Xiaorong³, QIAN Hong⁴, ZHANG Lan¹, YAN Peng¹, ZHANG Liping¹, ZHANG Xinfu², LI Xin^1,*, HAN Wenyan¹

1. Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China;
2. Horticulture Department, Qingdao Agricultural University, Qingdao 266109, China;
3. School of Economics and Management, Hebei University of Architecture, Zhangjiakou 075000, China;
4. Deqing Agricultural Technology Extension Center, Deqing 313200, China

Received:2020-03-03 Online:2020-11-25 Published:2020-12-02

摘要/Abstract

摘要： 茶树炭疽病是一种严重危害茶叶产量和品质的重要病害,在我国各大茶区均广泛发生。以龙井43和中茶108两个茶树品种为材料,研究炭疽病对茶树叶片光合速率、Rubisco活性、光合相关基因表达和过氧化氢(H₂O₂)含量的影响。结果表明:未接种茶树炭疽病病原菌的龙井43和中茶108的叶片光合速率无显著差异;接种炭疽病病原菌后,龙井43和中茶108的叶片光合速率均随着接种时间延长而显著降低,接种6 d后,龙井43和中茶108的叶片光合速率分别降低了61.76%和42.04%,Rubisco最大羧化速率(V_cmax)分别降低了33.78%和20.22%,RuBP最大再生速率(J_max)分别降低了37.67%和20.83%,Rubisco活性也显著下降;接种茶树炭疽病病原菌后光合相关基因表达下调,龙井43和中茶108叶片中CsRbcL的表达量分别降低47.08%和36.36%,CsRbcS的表达量分别降低34.99%和23.90%。此外,接种炭疽病病原菌后,龙井43和中茶108叶片中H₂O₂含量分别提高174.91%和96.69%。以上结果表明,龙井43比中茶108对炭疽病更为敏感,推测茶树炭疽病发生后叶片光合速率和Rubisco活性的降低可能与叶片中H₂O₂的过量累积有关。

关键词: 炭疽病, 茶树, 光合作用, 过氧化氢, 基因表达

Abstract: Anthracnose caused by Colletotrichum camelliae is an important foliar disease that severely damages the yield and quality of tea. It occurs widely in most of the tea production areas in China. In this study, Longjing 43 and Zhongcha 108 were used as experimental materials to explore the effects of anthracnose on photosynthetic rate, Rubisco activity, photosynthetic genes expression and H₂O₂ content in tea leaves. It was revealed that there was no significant difference in photosynthetic rate between Longjing 43 and Zhongcha 108 under mock treatment. However, after inoculation with the pathogen, the photosynthetic rate decreased significantly along with the post-inoculation time. At 6 days post-inoculation, the photosynthetic rate of Longjing 43 and Zhongcha 108 decreased by 61.76% and 42.04%, maximum carboxylation rate of Rubisco (V_cmax) decreased by 33.78% and 20.22%, and maximum rates of RuBP regeneration (J_max) decreased by 37.67% and 20.83%, respectively. The expression of photosynthetic genes decreased significantly after inoculation. Notably, the expression of CsRbcL in the leaves of Longjing 43 and Zhongcha 108 decreased by 47.08% and 36.36%, and CsRbcS decreased by 34.99% and 23.90%, respectively. In addition, the content of H₂O₂ in the leaves of Longjing 43 and Zhongcha 108 increased by 174.91% and 96.69%, respectively. The study further confirmed that Longjing 43 was more susceptible to anthracnose than Zhongcha 108. It was also suggested that the decrease of leaf photosynthetic rate and Rubisco activity after pathogen inoculation was mainly attributed to the excessive accumulation of H₂O₂ in tea leaves.

Key words: anthracnose, Camellia sinensis, photosynthesis, hydrogen peroxide, gene expression

中图分类号:

S435.711

张政, 王晓荣, 钱虹, 张兰, 颜鹏, 张丽平, 张新富, 李鑫, 韩文炎. 炭疽病对茶树叶片光合特性的影响[J]. 浙江农业学报, 2020, 32(11): 2020-2026.

ZHANG Zheng, WANG Xiaorong, QIAN Hong, ZHANG Lan, YAN Peng, ZHANG Liping, ZHANG Xinfu, LI Xin, HAN Wenyan. Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.)[J]. , 2020, 32(11): 2020-2026.

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炭疽病对茶树叶片光合特性的影响

Effects of anthracnose disease on photosynthetic characteristics in tea leaves (Camellia sinensis L.)

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