不同类型烟草种质的烟碱含量变化与相关基因表达水平

doi:10.3969/j.issn.1004-1524.2020.09.04

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1555-1563.DOI: 10.3969/j.issn.1004-1524.2020.09.04

不同类型烟草种质的烟碱含量变化与相关基因表达水平

徐秀红, 刘金亮, 李栋成, 刘仁祥^*

贵州大学烟草学院/贵州省烟草品质研究重点实验室,贵州贵阳 550025

收稿日期:2020-03-18 出版日期:2020-09-25 发布日期:2020-10-10
通讯作者: *刘仁祥,E-mail:1525135452@qq.com
作者简介:徐秀红(1980—),女,安徽宣城人,博士,副教授,研究方向为种质创新与利用、作物分子改良。E-mail:5725861@qq.com
基金资助:
贵州省烟草公司科技项目(201502,黔烟科〔2016〕11号);毕节市烟草公司项目(毕烟201652050024149号)

Analysis of nicotine content and related gene expression in different types of tobacco germplasm

XU Xiuhong, LIU Jinliang, LI Dongcheng, LIU Renxiang^*

College of Tobacco Science, Guizhou University/The Key Laboratory for Tobacco Quality of Guizhou Province, Guiyang 550025, China

Received:2020-03-18 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 为研究不同类型烟草种质的烟碱含量变化规律与相关基因的表达情况,以烤烟K326、香料烟巴斯玛、白肋烟TN90、晾烟马里兰为材料,采用荧光定量PCR技术,测定了烟草不同生育时期上部叶、中部叶、下部叶的烟碱含量与烟碱代谢过程中相关基因PMT、QPT、CYP82E5V2、A622的表达水平。结果表明,随着生育期的推进,烟碱含量缓慢上升,打顶后急剧增加,除巴斯玛打顶后还继续上升外,K326、TN90和马里兰烟均表现为:打顶后烟碱含量急剧增加,后缓慢下降,再渐趋平稳。不同类型烟草种质的PMT在不同生育期均有表达,苗期PMT表达量较高,其变化趋势与烟碱含量的基本一致;QPT在烟草发育前期表达量较高,而后下降,至打顶前1周表达量出现一个峰值,打顶后表达量持续增加,与烟碱含量变化呈正相关;CYP82E5V2表达量在不同生育时期均维持在较高水平,呈波浪型,生长旺期时表达量上升,打顶前后均出现峰值,但巴斯玛成熟期时其表达量下降,打顶前CYP82E5V2表达量与烟碱含量变化呈正相关,打顶后期至成熟期两者呈一定程度的负相关;K326和马里兰烟的A622表达量呈波浪型上升,至成熟时仍维持较高水平,而TN90和巴斯玛在打顶前后1周表达量较高,后表达下降再上升,成熟时表达量较低,与烟碱含量变化无明显一致性。

关键词: 烟草, 烟碱, 实时定量PCR, 基因表达

Abstract: In order to study the changes of nicotine content and expression of related genes in different types of tobacco germplasm, four tobacco types, including flue-cured tobacco K326, oriental tobacco Basma, burley tobacco TN90 and sun-cured tobacco Maryland were used as materials. Nicotine content in different leaves and the expression levels of nicotine metabolism related genes PMT, QPT, CYP82E5V2 and A622 were measured at different developmental stages. The results showed that with the passing of growth stage, the nicotine content increased slowly, and increased dramatically after topping. Except for Basma, the nicotine content continued to increase after topping, while in K326, TN90 and Maryland, the nicotine content increased sharply after topping, then decreased slowly, and gradually stabilized. PMT gene in different types of tobacco was expressed in different growth stages. Expression level of PMT was higher at the seedling stage, and its change trend was basically the same as that of the nicotine content. Expression level of QPT was higher in the early stage, then decreased, and reached a peak one week before topping. After topping, the expression level continued to increase, which was positively correlated with the change of nicotine content. Expression level of CYP82E5V2 remained at a high level in different growth stages, presenting a wave pattern, and increased in the bloom stage, and peaked before and after topping. However, expression level of CYP82E5V2 in Basma decreased when it was mature. Expression level of CYP82E5V2 was positively correlated with the change of nicotine content before the topping, and negatively correlated to a certain extent from the later topping stage to the mature stage. Expression level of A622 in K326 and Maryland rose in a wave-like manner, and remained at a high level at maturity, while in TN90 and Basma, expression level of A622 was higher one week before and after topping, then decreased, and increased again, the expression level was lower at maturity, and there was no obvious consistency with the change of nicotine content.

Key words: tobacco, nicotine, quantitative real-time PCR, gene expression

中图分类号:

S572

徐秀红, 刘金亮, 李栋成, 刘仁祥. 不同类型烟草种质的烟碱含量变化与相关基因表达水平[J]. 浙江农业学报, 2020, 32(9): 1555-1563.

XU Xiuhong, LIU Jinliang, LI Dongcheng, LIU Renxiang. Analysis of nicotine content and related gene expression in different types of tobacco germplasm[J]. , 2020, 32(9): 1555-1563.

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不同类型烟草种质的烟碱含量变化与相关基因表达水平

Analysis of nicotine content and related gene expression in different types of tobacco germplasm

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