浙江农业学报 ›› 2021, Vol. 33 ›› Issue (6): 965-973.DOI: 10.3969/j.issn.1004-1524.2021.06.01

• 作物科学 • 上一篇    下一篇

高粱种质资源出苗相关性状与SSR标记的关联分析

刘秀慧(), 邹桂花, 翟国伟, 刘合芹, 郑学强, 陈合云*()   

  1. 浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021
  • 收稿日期:2020-10-21 出版日期:2021-06-25 发布日期:2021-06-25
  • 通讯作者: 陈合云
  • 作者简介:*陈合云,E-mail: chy21@sina.com
    刘秀慧(1991—),女,山东聊城人,硕士,研究实习员,主要从事旱粮种质资源与遗产育种研究。E-mail: caas_liuxh@sina.com
  • 基金资助:
    国家自然科学基金(31571681)

Association analysis between SSR markers and germination-related traits in sorghum germplasm resources

LIU Xiuhui(), ZOU Guihua, ZHAI Guowei, LIU Heqin, ZHENG Xueqiang, CHEN Heyun*()   

  1. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2020-10-21 Online:2021-06-25 Published:2021-06-25
  • Contact: CHEN Heyun

摘要:

选用54对均匀分布在高粱全基因组的SSR标记对110份高粱种质资源进行检测,研究高粱中胚轴长、胚芽鞘长、根长和百粒重4个出苗相关性状与SSR标记的关联性,以期为选育耐深播的优异高粱资源提供理论支持和材料保障。结果表明,高粱中胚轴长与根长呈显著(P≤0.05)负相关,与胚芽鞘长呈极显著(P≤0.01)正相关。胚芽鞘长、根长和百粒重3个性状间存在极显著(P≤0.01)正相关;在54个SSR标记位点中,共检测到339个等位基因,平均每个位点检测到6.28个等位基因,平均有效等位基因数为2.67个,Shannon’s 信息指数平均值为1.129 9;结构分析将参试高粱种质主要分为3个亚群;关联分析共检测到9个标记与中胚轴长显著(P≤0.05)相关,单个位点解释的贡献率为11.18%~28.05%。5个标记与胚芽鞘长度显著(P≤0.05)相关,单个位点解释的贡献率为11.22%~18.12%。只有1个标记与根长显著(P≤0.05)相关,另检测到2个标记与百粒重显著相关。

关键词: 高粱, 出苗相关性状, SSR标记, 关联分析

Abstract:

Fifty-four pairs of simple sequence repeat (SSR) markers spanned uniformly over sorghum genome were selected to detect 110 sorghum germplasm resources. The relevances between SSR and germination-related traits (mesocotyl length, coleoptile elongate, root length, and hundred-grain weight) were studied in order to provide theoretical foundation and materials for screening elite deep-seeding tolerance in sorghum. The results showed that there were significantly (P≤0.05) negative correlation between mesocotyl length and root length, and significantly (P≤0.01) positive correlation between mesocotyl length and coleoptile length. Significantly (P≤0.01) positive correlations were found among coleoptile length, root length, and hundred-grain weight. A total of 339 alleles were identified at all of 54 SSR markers loci, with an average of 6.28 alleles per marker locus and 2.67 effective alleles. The average values of Shannon’s information index were 1.129 9. All of the sorghum germplasms were divided into three subgroups by structural analysis. Nine sites had significant (P≤0.05) correlation with mesocotyl length, whose phenotypic contribution rate was from 11.18%-28.05%. Five sites had significant (P≤0.05) correlation with coleoptile elongate, whose phenotypic contribution rate were 11.22%-18.12%. Only one site had significant (P≤0.05) correlation with root length. Two sites had significant correlation with hundred-grain weight.

Key words: sorghum, seedling-emergence related traits, SSR markers, association analysis

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