精准定时输精对后备母猪早期胚胎发育与繁殖性能的影响

doi:10.3969/j.issn.1004-1524.2021.05.03

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (5): 794-800.DOI: 10.3969/j.issn.1004-1524.2021.05.03

精准定时输精对后备母猪早期胚胎发育与繁殖性能的影响

朱志伟¹^,²(), 陈晓宇¹, 于福先¹, 张樑¹, 黄菁¹^,², 王志刚¹, 赖建兵³, 沈顺新⁴, 殷文彬², 潘建治¹^,²^,^*()

1.浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021
2.宁波旷代畜牧科技有限公司,浙江宁波 315181
3.浙江欣宏源生态农业有限公司,浙江青田 313900
4.安吉正新牧业有限公司,浙江安吉 313300

收稿日期:2020-12-12 出版日期:2021-05-25 发布日期:2021-05-25
通讯作者: 潘建治
作者简介:*潘建治,E-mail:jzpan9@126.com
朱志伟(1970—),男,浙江上虞人,硕士,副研究员,主要从事动物繁殖调控与胚胎工程技术研究。E-mail:zhuzw1970@163.com
基金资助:
国家重点研发计划(2017YFD0501901-3);浙江省重大科技专项重大农业项目(2016C02054-6);浙江省省长基金项目(2020R30A60C01)

Effect of fixed-time artificial insemination on earlier embryonic development and reproduction performance in gilts

ZHU Zhiwei¹^,²(), CHEN Xiaoyu¹, YU Fuxian¹, ZHANG Liang¹, HUANG Jing¹^,², WANG Zhigang¹, LAI Jianbing³, SHEN Shunxin⁴, YIN Wenbin², PAN Jianzhi¹^,²^,^*()

1. Institute of Virology and Biotechnology, Zhejiang Acadeny of Agricultural Sciences, Hangzhou 310021, China
2. Ningbo Kuagndai livestock Husbandry Technology Co.,Ltd., Ningbo 315181, China
3. Zhejiang Xinhongyuan Ecological Agriculture Co.,Ltd., Qingtian 313900, China
4. Anji Zhengxin Animal Husbandry Co.,Ltd., Anji 313300, China

Received:2020-12-12 Online:2021-05-25 Published:2021-05-25
Contact: PAN Jianzhi

摘要/Abstract

摘要：

为探讨精准定时输精(fixed-time artificial insemination, FTAI)对后备母猪早期胚胎发育和繁殖性能的影响,试验1选取自主培育的金乌猪后备母猪43头,随机分为2组,其中,对照组24头,为自然发情,观察后人工配种(artificial insemination, AI);试验组19头,为精准定时输精组。分别于0 h(停喂烯丙孕素)、42 h [肌注孕马血清促性腺激素(pregnant mare serum gonadotropin, PMSG)]、122 h [肌注促性腺激素释放激素(gonadotropin releasing hormone, GnRH)]静脉采血,检测孕酮(progesteron, P4)、雌二醇(estradiol, E2)、促卵泡激素(follicle-stimulating hormone, FSH)和黄体生成素(luteinizing hormone, LH)的含量;在配种后33 h,分别屠宰对照组和试验组4头后备母猪,回收受精卵进行体外培养,其余后备母猪继续饲养并进行分娩与产仔情况追踪。结果显示,从停喂烯丙孕素至42 h和122 h,P4值呈下降趋势但差异不显著(P>0.05);停喂烯丙孕素至停喂后42 h,E2值从80 pmol·L^-1显著(P<0.05)下降至58.3 pmo·L^-1,而在PMSG作用后又急剧升至134.07 pmol·L^-1(P<0.01);FSH值未见显著变化(P>0.05);停喂烯丙孕素至停喂后42 h,LH值由0.09 IU·L^-1升至0.12 IU·L^-1(P>0.05),PMSG作用后又降至0.09 IU·L^-1(P>0.05),呈先升后降的趋势,符合正常生理周期的变化趋势,表明用药合理无副作用。屠宰后卵巢排卵点计数结果显示,试验组17.5枚,对照组15.25枚,差异不显著(P>0.05);试验组平均窝均总产仔数、活仔数与对照组相比未见显著差异,但相对提高1.05头。试验2选取大长后备母猪189头,处理方式同试验1,随机分为试验组94头、对照组95头,试验组活仔较对照组约提高0.58头(P>0.05),断奶成活率提高2.99百分点。综上所述,精准定时输精程序处理符合母猪生理发情周期特质,可增加排卵数且对早期胚胎发育无不良影响,在提高后备母猪的利用率、增加窝均活仔数和提高仔猪断奶成活率等方面都有一定的积极作用,值得进一步优化研究和推广应用。

关键词: 精准定时输精, 后备母猪, 胚胎, 繁殖性能

Abstract:

To explore the effect of fixed-time artificial insemination (FTAI) on earlier embryonic development and fertility of gilts, in experiment 1, totally 43 gilts (Jinwu pig) were randomly divided into control (n=24) and FTAI groups (n=19). The control group were natural estrus, and performed artificial insemination (AIs) after a standing reflex, and the FTAI group were performed AIs after administration of altrenogest+pregnant mare serum gonadotropin (PMSG)+gonadotropin releasing hormone (GnRH) (ALT+PMSG+GnRH). Blood samples were collected for detecting of the concentration of progesteron (P4), estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) at 0 h (stop feeding altrenogest), 42 h (intramuscular injection of PMSG) and 122 h (intramuscular injection of GnRH). Four gilts of each group were slaughtered at 33 h after AIs, and the fertilized eggs were recovered for in vitro culture, the remaining gilts were used to observe the fertility. The results showed that concentration of progesterone were downregulated from 0 h to 122 h, but there was no significant difference(P>0.05). Estradiol levels were dropped from 80 pmol·L^-1 to 58.3 pmol·L^-1 (P<0.05) during 0 h to 42 h, but dramatically increased to 134.07 pmol·L^-1 from 42 h to 122 h (P<0.01). FSH value showed no significant difference (P>0.05). The concentration of luteinizing hormone increased gradually from 0.09 IU·L^-1 to 0.12 IU·L^-1, and decreased to 0.09 IU·L^-1 at these three time points, showing a up and down trend, which conformed the trend of normal physiological cycle, indicating the administration was reasonable and had no side effects. The ovaries were collected from the control and FTAI groups at 33 h after AIs, the ovulation point were counted, showing that there was no significant difference (P>0.05) between FTAI group (17.5) and control group (15.25). Meanwhile, compared with the control group, the litter size and alive litter size of FTAI group did not show significant difference (P>0.05), but the litter size and alive litter size of FTAI group were all increase of 1.05 heads relatively. In experiment 2, totally 189 gilts (Yorkshire×Landrace) were randomly divided into control (n=95) and FTAI groups (n=94). The alive litter size of FTAI group was increased 0.58 heads, and the weaning survival rate increased 2.99 percentage point compared with the control group. In summary, the administration of FTAI procedure was conforming to the characteristics of gilts physiological estrous cycle, and might increase the ovulation number without adverse effects on the earlier embryos development. Meanwhile, the FTAI procedure had a positive effect on improving the utilization of gilts and increasing the alive litter size and survival rate of weaning piglets to a certain degree, which was worthy of further optimization, promotion and application.

Key words: fixed-time artificial insemination, gilt, embryo, reproduction performance

中图分类号:

S828

朱志伟, 陈晓宇, 于福先, 张樑, 黄菁, 王志刚, 赖建兵, 沈顺新, 殷文彬, 潘建治. 精准定时输精对后备母猪早期胚胎发育与繁殖性能的影响[J]. 浙江农业学报, 2021, 33(5): 794-800.

ZHU Zhiwei, CHEN Xiaoyu, YU Fuxian, ZHANG Liang, HUANG Jing, WANG Zhigang, LAI Jianbing, SHEN Shunxin, YIN Wenbin, PAN Jianzhi. Effect of fixed-time artificial insemination on earlier embryonic development and reproduction performance in gilts[J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 794-800.

图/表 5

图1 停喂烯丙孕素后不同时间检测血液中各激素含量所有数据来自于14次独立重复试验结果,不同字母表示差异显著(P<0.05),相同字母表示差异不显著(P>0.05)。

Fig.1 Levels of different hormones at different time points after cessation of allergenin The data wa based on fourteen independent experiments, the different letter indicated significant difference (P<0.05), and the same letters meant no significant difference (P>0.05).

表1 精准定时输精程序处理对后备母猪早期胚胎发育的影响

Table 1 Effects of FTAI on early embryo development in gilts

组别 Group	屠宰数量 Slaughter number	头均排卵数量 Average number of ovulation	回收胚数量 Recycle number	单细胞率 Monoplast rate/%	2-8细胞率 Rate of 2-8 cell/%	囊胚率 Rate of blastocyst/%
定时输精组FTAI group	4	17.50±1.73 a	42 a	14.28 a	85.72 a	75.00 a
对照组Control group	4	15.25±4.03 a	36 a	13.88 a	86.11 a	74.19 a

图2 胚胎早期发育情况 A、B、C:试验组胚胎,从左到右依次为2细胞期、4细胞期、囊胚期;D、E、F:对照组胚胎,从左到右依次为:2细胞期、4细胞期、囊胚期。标尺=100 μm。

Fig.2 Early embryonic development A, B, C: The embryo of FTAI group: 2 cell stage, 4 cell stage and blastula stage; D, E, F: The embryo of control group: 2 cell stage, 4 cell stage and blastula stage. Bar=100 μm.

表2 精准定时输精程序处理对金乌猪繁殖性能的影响

Table 2 Effect of FTAI on reproductive performance of Jinwu pig

组别 Groups	参繁数量 Number of gilts	配种数量 Number of insemination	参配率 Rate of insemination/%	分娩数量 Number of parturition	分娩率 Parturition rate/%	窝均总产仔数 Litter size	窝均活仔数 Alive litter size
定时输精组FTAI group	15	15	100 a	13	86.66 a	10.69±1.75 a	10.46±1.56 a
对照组Control group	20	18	90 a	17	85.00 a	9.64±3.18 a	9.41+2.95 a

表3 精准定时输精程序对大长母猪繁殖性能的影响

Table 3 Effect of FTAI on reproductive performance of large-white sows

组别 Groups	参繁数量 Number of gilts	参配率 Rate of insemination/%	分娩率 parturition rate/%	窝均总产仔数 Litter size	窝均活仔数 Alive litter size	断奶仔 Weaned litter size	断奶成活率 Weaning survival rate/%
定时输精组FTAI group	94	100 a	93.61 a	12.06±3.69 a	11.10±3.61 a	898	91.91 a
对照组Control group	95	93.68 a	89.47 a	11.64±3.85 a	10.52±3.95 a	795	88.92 a

参考文献 11

[1]	翁士乔. 母猪定时输精技术[J]. 国外畜牧学(猪与禽), 2016,36(6):1-3.
	WENG S Q. Sow timed infusion technology[J]. Animal Science Abroad (Pigs and Poultry), 2016,36(6):1-3.(in Chinese)
[2]	DE RENSIS F, KIRKWOOD R N. Control of estrus and ovulation: fertility to timed insemination of gilts and sows[J]. Theriogenology, 2016,86(6):1460-1466. DOI URL
[3]	吴俊辉, 曹丁壬, 刘艳婷, 等. 家畜定时输精技术研究进展与应用现状[J]. 中国畜牧兽医, 2016,43(7):1826-1833.
	WU J H, CAO D R, LIU Y T, et al. Research advances and application status of timed artificial insemination (TAI) in farm animals[J]. China Animal Husbandry & Veterinary Medicine, 2016,43(7):1826-1833.(in Chinese with English abstract)
[4]	郭晓飞, 张金龙, 李义海, 等. 猪定时输精技术研究进展[J]. 中国畜牧杂志, 2019,55(9):28-33.
	GUO X F, ZHANG J L, LI Y H, et al. Research progress on technology of timed artificial insemination in pig[J]. Chinese Journal of Animal Science, 2019,55(9):28-33.(in Chinese with English abstract)
[5]	GUTHRIE H D, BOLT D J. Pituitary and ovarian hormone secretion and ovulation in gifts injected with gonadotropins during and after oral administration of progesterone agonist (altrenogest)₁[J]. Biology of Reproduction, 1985,33(3):679-689. DOI URL
[6]	GUTHRIE H D, BOLT D J. Changes in plasma follicle-stimulating hormone, luteinizing hormone, estrogen and progesterone during growth of ovulatory follicles in the pig[J]. Domestic Animal Endocrinology, 1990,7(1):83-91. DOI URL
[7]	DEGENSTEIN K L, O’DONOGHUE R, PATTERSON J L, et al. Synchronization of ovulation in cyclic gilts with porcine luteinizing hormone (pLH) and its effects on reproductive function[J]. Theriogenology, 2008,70(7):1075-1085. DOI URL
[8]	RAVAGNANI G M, MARTINEZ C H G, CARNEVALE R F, et al. Effects of oestrous synchronization with altrenogest in gilts on endometrial and embryonic characteristics[J]. Animal, 2020,14(9):1899-1905. DOI URL
[9]	WANG Z, LIU B S, WANG X Y, et al. Effects of altrenogest on reproductive performance of gilts and sows: a meta-analysis[J]. Animal Reproduction Science, 2018,197:10-21. DOI URL
[10]	LOPES T, A BOLARÍN, E MARTÍNEZ, et al. Altrenogest treatment before weaning improves litter size in sows[J]. Reproduction in Domestic Animals, 2017,52:75-77. DOI URL
[11]	吴宝云, 李贺, 张衡, 等. 不同定时输精程序对后备母猪繁殖性能的影响[J]. 中国畜牧杂志, 2018,54(7):55-58.
	WU B Y, LI H, ZHANG H, et al. Effects of different fixed-time artificial insemination programs on the reproductive performance of gilts[J]. Chinese Journal of Animal Science, 2018,54(7):55-58.(in Chinese with English abstract)

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精准定时输精对后备母猪早期胚胎发育与繁殖性能的影响

Effect of fixed-time artificial insemination on earlier embryonic development and reproduction performance in gilts

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