免疫增强剂对猪伪狂犬灭活疫苗的免疫增强作用

doi:10.3969/j.issn.1004-1524.2016.11.04

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (11): 1828-1833.DOI: 10.3969/j.issn.1004-1524.2016.11.04

免疫增强剂对猪伪狂犬灭活疫苗的免疫增强作用

耿文学¹, 唐波², 华涛², 侯继波², 张道华², 许家荣^{1, *}

1.南京农业大学动物医学院,江苏南京 210095;
2.江苏省农业科学院国家兽用生物制品工程技术研究中心,江苏南京 210014

收稿日期:2016-04-18 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 许家荣,E-mail:xjr@njau.edu.cn
作者简介:耿文学(1990—),男,山东淄博人,硕士研究生,从事预防传染病学研究。E-mail:13951692816@163.com
基金资助:
江苏省农业科技自主创新基金项目[CX(11)2047]; 农业公益性行业科研专项(201303046); 江苏省优质学科(20150101)

Improved immune efficiency of inactivated vaccine of pseudorabies virus by immunopotentiator

GENG Wen-xue¹, TANG Bo², HUA Tao², HOU Ji-bo², ZHANG Dao-hua², XU Jia-rong^{1, *}

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2016-04-18 Online:2016-11-15 Published:2016-11-16

摘要/Abstract

摘要： 为了评价免疫增强剂VA5对猪伪狂犬病毒灭活疫苗的免疫增强作用,将VA5与灭活疫苗混合并制成疫苗。通过常规Bartha-K61株灭活疫苗、含免疫增强剂灭活疫苗和Bartha-K61活疫苗3组免疫效力对比试验,首次免疫后14和35 d对猪进行采血,并且进行中和实验以及对相关细胞因子进行检测。结果表明,该免疫增强剂能够显著地提高猪伪狂犬灭活疫苗血清中抗体产生,同时能显著提高猪外周血淋巴细胞(PBMC)中IL-2和IFN-γ mRNA表达。交叉中和试验表明,VA5能够显著地提高猪伪狂犬灭活疫苗对伪狂犬流行变异株的中和作用。试验表明,VA5能够显著提升猪伪狂犬病毒灭活疫苗体液与细胞免疫,为研制免疫效果更好的灭活疫苗提供了依据。

关键词: 免疫增强剂, 伪狂犬病毒灭活疫苗, Bartha-K61株, 免疫效力

Abstract: To evaluate the effect of immunopotentiator VA5 on immunity potency of inactive vaccine of pseudorabies virus (PRV), inactive vaccine was mixed with VA5. The comparison test of the immunity potency was conducted by using conventional inactive vaccine of Bartha-K61 strain of PRV, inactive vaccine mixed with VA5 and attenuated vaccine of Bartha-K61 strain. Blood samples were collected at 14 and 35 days after the first immunization, respectively. Neutralization experiments and cytokine detection were performed. The results showed that, compared with live and inactive vaccine of Bartha-K61 strain of PRV, the inactive vaccine with VA5 could induce higher neutralizing antibody against the heterologous strain of PRV and increase the expression levels of IL-2 and IFN-γ mRNA in porcine peripheral blood lymphocytes (PBMC). Neutralization test showed that VA5 could significantly increase the level of neutralizing antibody against the epidemic variation strain of PRV. This experiment showed that VA5 could improve the humoral and cellular immunity of inactive PRV vaccine, and provide a basis for paving the better immune effect of inactivated PRV vaccine.

Key words: immunopotentiator, inactive PRV vaccine, Bartha-K61 strain, immunity potency

中图分类号:

S851.35

耿文学, 唐波, 华涛, 侯继波, 张道华, 许家荣. 免疫增强剂对猪伪狂犬灭活疫苗的免疫增强作用[J]. 浙江农业学报, 2016, 28(11): 1828-1833.

GENG Wen-xue, TANG Bo, HUA Tao, HOU Ji-bo, ZHANG Dao-hua, XU Jia-rong. Improved immune efficiency of inactivated vaccine of pseudorabies virus by immunopotentiator[J]. , 2016, 28(11): 1828-1833.

参考文献

[1] OLIVER R E. Aujeszky’s disease[J]. Australian Veterinary , 1989, 66(3):432-433.
[2] LUO Y, LI N, CONG X, et al. Pathogenicity and genomic characterization of a pseudorabies virus variant isolated from Bartha-K61-vaccinated swine population in China[J]. Veterinary Microbiology , 2014,174(1/2):107-115.
[3] ZHANG C, GUO L, JIA X, et al. Construction of a triple gene-deleted Chinese Pseudorabies virus variant and its efficacy study as a vaccine candidate on suckling piglets[J]. Vaccine , 2015, 33(21):2432-2437.
[4] GU Z, DONG J, WANG J, et al. A novel inactivated gE/gI deleted pseudorabies virus (PRV) vaccine completely protects pigs from an emerged variant PRV challenge[J]. Virus Research , 2015, 195:57-63.
[5] AN T Q, PENG J M, TIAN Z J, et al. Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012[J]. Emerging Infectious Diseases , 2013, 19(11):1749-1755.
[6] KAWAI T, AKIRA S.The roles of TLRs, RLRs and NLRs in pathogen recognition[J]. International Immunology , 2009, 21(4):317-337.
[7] FISCHER T, BÜTTNER M, RZIHA H J. T helper 1-type cytokine transcription in peripheral blood mononuclear cells of pseudorabies virus ( Suid herpesvirus 1)-primed swine indicates efficient immunization[J]. Immunology , 2000, 101(3):378-387.
[8] 唐应华,陆吉虎,吴培培,等.免疫增强剂提高禽流感疫苗效力的研究[J].江苏农业学报,2014,30(2):344-348.
TANG Y H, LU J H, WU P P, et al.Evaluation of immunopotentiators for improving the efficacy of avian influenza vaccine[J]. Jiangsu Journal of Agricultural Sciences , 2014, 30(2):344-348.(in Chinese with English abstract)
[9] 毛明光,尹立杰,王印庚,等.聚肌胞对雏鸡新城疫抗体水平的影响[J]. 中国家禽,2009,31(14):26-28.
MAO M G, YIN L J, WANG Y G, et al. Effect of polyinosinic-polycytidylic acid on antibody response to newcastle disease vaccine in chickens[J]. China Poultry , 2009, 31(14):26-28.(in Chinese with English abstract)
[10] ZHANG L L, BAI J, LIU J, et al. Toll-like receptor ligands enhance the protective effects of vaccination against porcine reproductive and respiratory syndrome virus in swine[J]. Veterinary Microbiology , 2013, 164 (3/4):253-260.
[11] TANG Y H,LU J H,WU P P,et al. Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens[J]. Veterinary Microbiology , 2014, 172(1/2):120-128.
[12] IWASAKI A, MEDZHITOV R. Regulation of adaptive immunity by the innate immune system[J]. Science , 2010, 327(5963):291-295.
[13] GORDON S. Pattern recognition receptors: doubling up for the innate immune response[J]. Cell , 2002, 111(7):927-930.
[14] 司兴奎,郭鑫,杨汉春.猪圆环病毒2型感染后猪外周血淋巴细胞IL-2、IL-4、IL-10、IL-12p40、IFN-γ和TNF-α mRNA转录的变化[J]. 畜牧兽医学报,2009,40(1):78-82.
SI X K, GUO X, YANG H C. The effects of PCV2 experimental infection on transcriptional levels of IL-2, IL-4, IL-10, IL-12p40, IFN-γ and TNF-α mRNA of peripheral blood lymphocytes[J]. Acta Veterinaria et Zootechnica Sinica , 2009, 40(1): 78-82. (in Chinese with English abstract)

免疫增强剂对猪伪狂犬灭活疫苗的免疫增强作用

Improved immune efficiency of inactivated vaccine of pseudorabies virus by immunopotentiator

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	陈章, 刘晓露, 姚焱彬, 吴琼娟, 孙裴, 魏建忠, 李东风, 李郁. 猪丹毒丝菌灭活疫苗免疫佐剂的筛选[J]. 浙江农业学报, 2019, 31(11): 1803-1811.
[2]	沈锦玉;刘问;曹铮;尹文林;沈智华;钱冬;吴颖蕾. 免疫增强剂对中华绒螯蟹免疫功能的影响[J]. , 2004, 16(1): 0-29.