玉米ZmREM基因的克隆与逆境胁迫后表达分析

doi:10.3969/j.issn.1004-1524.2016.11.03

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (11): 1822-1827.DOI: 10.3969/j.issn.1004-1524.2016.11.03

玉米ZmREM基因的克隆与逆境胁迫后表达分析

瓮巧云¹, 赵彦敏², 张贺¹, 宋晋辉¹, 马海莲¹, 袁进成¹, 刘颖慧^{1, *}

1.河北北方学院农林科技学院,河北张家口 075000;
2.张家口市广播电视大学,河北张家口 075000

收稿日期:2016-03-31 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 刘颖慧,E-mail:leely519@126.com
作者简介:瓮巧云(1979—),女,河北定州人,博士,副教授,从事植物抗逆机制研究。E-mail:nkxwqy@163.com
基金资助:
国家自然科学基金(31101155); 河北北方学院重大项目(ZD201407,ZD201305); 河北省科技厅项目(13226326)

Cloning of ZmREM and its expression analysis in maize under stress

WENG Qiao-yun¹, ZHAO Yan-min², ZHANG He¹, SONG Jin-hui¹, MA Hai-lian¹, YUAN Jin-cheng¹, LIU Ying-hui^{1, *}

1. College of Agriculture and Forestry, Hebei North University, Zhangjiakou 075000, China;
2. Zhangjiakou Radio & TV University, Zhangjiakou 075000, China

Received:2016-03-31 Online:2016-11-15 Published:2016-11-16

摘要/Abstract

摘要： B3超家族基因是植物中特有的一类转录因子,参与植物生长发育、形态建成及抗逆境胁迫等过程。从玉米黄早4中获得一个含有2个B3-DNA结合域的基因,为B3超家族基因,命名为ZmREM。该基因全长1 047 bp,编码含有349个氨基酸的蛋白。Blast比对结果表明,ZmREM和谷子含有B3结构域蛋白、高粱的假定蛋白相似性分别为81%和84%。ZmREM基因是组成型表达,在根中表达量最高。同时ZmREM基因的转录水平可以为干旱、盐、冷和热所诱导。因此,ZmREM可能参与玉米多种非生物胁迫应答途径。

关键词: 玉米, REM转录因子, 基因克隆, 非生物胁迫

Abstract: The plant-specific B3 superfamily constituted a large transcription factor superfamily. They play central roles in plant development, morphogenesis and stress-resistance. Taking total RNA of maize as template, REM gene was cloned by use the method of reverse transcription polymerase chain reaction (RT-PCR). Length of 1 047 bp cDNA sequence that encoding 349 amino acids was obtained. CD search result showed that it contained two B3-DNA domains and was named as ZmREM. Blast result indicated that ZmREM had 84% and 81% homology with Sorghum bicolor hypothetical protein and Setaria italica B3 domain-containing protein. In various tissues, gene expression in root was the highest. The expression of ZmREM in maize was induced by heat, high-salt, cold and PEG treatment. These results suggested that ZmREM might be a stress related gene of maize.

Key words: maize (Zea mays L.), REM transcription factor, gene cloning, abiotic stress

中图分类号:

S513

瓮巧云, 赵彦敏, 张贺, 宋晋辉, 马海莲, 袁进成, 刘颖慧. 玉米ZmREM基因的克隆与逆境胁迫后表达分析[J]. 浙江农业学报, 2016, 28(11): 1822-1827.

WENG Qiao-yun, ZHAO Yan-min, ZHANG He, SONG Jin-hui, MA Hai-lian, YUAN Jin-cheng, LIU Ying-hui. Cloning of ZmREM and its expression analysis in maize under stress[J]. , 2016, 28(11): 1822-1827.

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玉米ZmREM基因的克隆与逆境胁迫后表达分析

Cloning of ZmREM and its expression analysis in maize under stress

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