浙江农业学报 ›› 2016, Vol. 28 ›› Issue (11): 1915-1921.DOI: 10.3969/j.issn.1004-1524.2016.11.17

• 环境科学 • 上一篇    下一篇

不同碳源促进污染水体氮素转化的微生态过程

赵佩文1, 2, 王新2, 吴逸飞2, 姚晓红2, 柳永2, 孙宏2, 葛向阳1, 汤江武2, *   

  1. 1.华中农业大学 生命科学与技术学院,湖北 武汉 430070;
    2.浙江省农业科学院 植物保护与微生物研究所,浙江 杭州 310021
  • 收稿日期:2016-03-14 出版日期:2016-11-15 发布日期:2016-11-16
  • 通讯作者: 汤江武,E-mail:tangjiangwu@sina.com
  • 作者简介:赵佩文(1991—),女,湖北武汉人,硕士研究生,主要研究方向为微生物生态学。E-mail:332726781@qq.com
  • 基金资助:
    浙江省公益技术应用研究项目(2015C33046); 浙江省重大科技专项重点社发项目(2015C03004); 浙江省农科院创新提升工程项目; 温州市水体污染控制与治理科技创新项目(S20140021)

Microbial ecological process of nitrogen transformation accelerated by different organic carbon in polluted water

ZHAO Pei-wen1, 2, WANG Xin2, WU Yi-fei2, YAO Xiao-hong2, LIU Yong2, SUN Hong2, GE Xiang-yang1, TANG Jiang-wu2, *   

  1. 1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070,China;
    2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
  • Received:2016-03-14 Online:2016-11-15 Published:2016-11-16

摘要: 在试验条件下,向氮污染河道水体中C/N按15∶1补加甲醇、乙醇、乙酸钠、柠檬酸钠、葡萄糖、蔗糖、麦芽糖、羧甲基纤维素钠,检测水体氨氮、亚硝酸盐、硝酸盐、总氮的变化,以及在此过程中水体细菌胞外酶活性、细菌群落结构的变化。结果显示,除甲醇、羧甲基纤维素钠外,补加有机碳源显著降低了水体氨氮的含量,水体β-葡萄糖苷酶活性在碳源补加后快速增加,补加葡萄糖、蔗糖、麦芽糖的水体中细菌群落结构明显异于其他处理,表明添加有机碳源可显著加快污染水体中氮素的转化,水体微生物在此过程中起到关键作用。水体细菌群落结构和代谢活性对不同类型有机碳源有着不同的响应,因而添加不同有机碳源对水体氮素转化具有不同的促进作用。

关键词: 有机碳源, 污染水体, 细菌群落, PCR-DGGE, 细菌代谢活性

Abstract: In the present study, methanol, ethanol, sodium acetate, sodium citrate, glucose, sucrose, maltose, and CMC-Na was added in nitrogen-polluted urban river water with C/N of 15∶1. Variation of ammonia, nitrite, nitrate, total nitrogen were detected by national standard method, β-glucosidase activity were determined using the fluorogenic model substrates, and the change of bacterial community structure were analyzed by PCR-DGGE. It was shown that contents of ammonia decreased significantly after organic carbon sources addition except methanol or CMC-Na. Addition of organic carbon sources increased β-glucosidase activity. Bacterial community structure differed obviously with addition of glucose, sucrose and maltose. The results indicated that addition of organic carbon could accelerate transformation of nitrogen in the nitrogen-polluted water, in which microbes played key role. Bacterial community and metabolic activity had different responses to varied organic carbons, which would lead to different strength on nitrogen transformation acceleration.

Key words: organic carbon, polluted water, bacterial community, PCR-DGGE, bacterial metabolic activity

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