利用微卫星标记分析雁荡土鸡及其他3个鸡群体遗传多样性

doi:10.3969/j.issn.1004-1524.2017.07.03

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (7): 1070-1076.DOI: 10.3969/j.issn.1004-1524.2017.07.03

利用微卫星标记分析雁荡土鸡及其他3个鸡群体遗传多样性

曾涛¹, 孙思维², 田勇¹, 陈黎¹, 卢立志¹, 干贤通³, 干方本³, 吴春琴^{2, *}

1.浙江省农业科学院畜牧兽医研究所,浙江杭州 310021;
2.温州科技职业学院动物科学系,浙江温州 325006;
3.浙江绿雁农业开发有限公司,浙江乐清325615

收稿日期:2017-01-12 出版日期:2017-07-20 发布日期:2017-07-24
通讯作者: 吴春琴,E-mail:nkywcq@163.com
作者简介:曾涛(1987—),男,安徽安庆人,博士,助理研究员,主要从事家禽遗传育种研究。E-mail:zengtao4009@126.com
基金资助:
浙江省农业科技成果转化资金资助项目(2013-101); 温州市种子种苗科技创新专项项目(N20120031); 浙江省科学技术厅重大科技专项重大农业项目(2014C02001)

Genetic diversity of Yandang chicken and other three chicken populations using microsatellite markers

ZENG Tao¹, SUN Siwei², TIAN Yong¹, CHEN Li¹, LU Lizhi¹, GAN Xiantong³, GAN Fangben³, WU Chunqin^{2, *}

1.Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Department of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China;
3. Zhejiang Lvyan Agricultural Department Co., Ltd., Yueqing 325615, China

Received:2017-01-12 Online:2017-07-20 Published:2017-07-24

摘要/Abstract

摘要： 为探讨浙江省不同地方鸡群体的遗传多样性及遗传距离,促进浙江境内鸡群体遗传资源的合理利用和保护,本试验利用STR分型技术对雁荡麻鸡Ⅰ系、Ⅱ系、萧山鸡、龙游麻鸡和仙居鸡5个群体(440只个体)在28个位点上的遗传多样性进行了检测。结果表明,所有位点在5个群体中的平均有效等位基因(Ne)、期望杂合度(He)和多态信息含量(PIC)分别为3.709 0、0.664 1和0.621 5,其中龙游麻鸡的平均Ne、He和PIC最高(3.941 9、0.702 2和0.661 3);仙居鸡最低(3.505 1、0.653 9和0.609 3);雁荡麻鸡2个品系平均值处于龙游麻鸡和仙居鸡之间(Ⅰ系分别为3.785 5、0.670 6和0.628 8,Ⅱ系分别为3.695 7、0.654 6和0.611 4)。遗传距离和系统聚类结果显示,5个鸡群体聚为3类,雁荡麻鸡2个群体为一类,仙居鸡为一类,萧山鸡与龙游麻鸡为一类。雁荡麻鸡Ⅰ系和Ⅱ系是浙江境内不同于仙居鸡、萧山鸡和龙游麻鸡而单独存在的一个品种。

关键词: 鸡, 微卫星, 遗传多样性, 遗传距离

Abstract: In order to study the chicken populations' genetic diversity and relationship and promote the rational use and protection of the chicken populations genetic resources in Zhejiang Province, 28 microsatellite loci were tested in the 5 chicken populations, which were Yandang chicken I, Yandang chicken II, Xiaoshan chicken, Longyou chicken and Xianju chicken, totally 440 samples. The results showed that the average effective number of alleles (Ne) was 3.709 0, the average expected heterozygosity (He) was 0.664 1, and the mean polymorphic information content (PIC) was 0.621 5. In addition, the Ne, He and PIC of Longyou chicken were the highest (3.941 9, 0.702 2 and 0.661 3). The Ne, He and PIC of Xianju chicken were the lowest (3.505 1, 0.653 9 and 0.609 3). The Ne, He and PIC of Yandang chicken were between the two populations (Ⅰ: 3.785 5, 0.670 6 and 0.628 8; Ⅱ: 3.695 7, 0.654 6 and 0.611 4). Phylogenetic tree analysis based on Nei's genetic distance revealed that Yandang chicken (Ⅰ and Ⅱ), Xianju chicken, Xiaoshan chicken and Longyou chicken was clustered into threes group separately. It indicated that Yandang chicken was a separate breed which is different from Xiaoshan chicken, Longyou chicken and Xianju chicken.

Key words: chicken, microsatellite, genetic diversity, genetic distance

中图分类号:

S831.2

曾涛, 孙思维, 田勇, 陈黎, 卢立志, 干贤通, 干方本, 吴春琴. 利用微卫星标记分析雁荡土鸡及其他3个鸡群体遗传多样性[J]. 浙江农业学报, 2017, 29(7): 1070-1076.

ZENG Tao, SUN Siwei, TIAN Yong, CHEN Li, LU Lizhi, GAN Xiantong, GAN Fangben, WU Chunqin. Genetic diversity of Yandang chicken and other three chicken populations using microsatellite markers[J]. , 2017, 29(7): 1070-1076.

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利用微卫星标记分析雁荡土鸡及其他3个鸡群体遗传多样性

Genetic diversity of Yandang chicken and other three chicken populations using microsatellite markers

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