浙江农业学报 ›› 2017, Vol. 29 ›› Issue (12): 2023-2031.DOI: 10.3969/j.issn.1004-1524.2017.12.10

• 园艺科学 • 上一篇    下一篇

铁线莲属植物叶绿体微卫星引物开发及其遗传分析

陈文超, 苏琬涵, 刘志高*, 季梦成   

  1. 浙江农林大学 风景园林与建筑学院,浙江 临安 311300
  • 收稿日期:2017-05-12 出版日期:2017-12-20 发布日期:2018-01-08
  • 通讯作者: 刘志高,E-mail:vzhigao@sina.com
  • 作者简介:陈文超(1995—),男,浙江杭州人,园林专业本科生。E-mail:80576133@qq.com
  • 基金资助:
    浙江省农业(林木)新品种选育重大科技专项子课题(2016C02056-13-4); 浙江农林大学学生科研训练项目(104-2013200015)

Development and genetic analysis of novel chloroplast microsatellite markers of Clematis

CHEN Wenchao, SU Wanhan, LIU Zhigao*, JI Mengcheng   

  1. College of Landscape Architecture, Zhejiang A & F University, Lin'an 311300, China
  • Received:2017-05-12 Online:2017-12-20 Published:2018-01-08

摘要: 对GeneBank数据库中Clematis fusca var. coreana叶绿体全基因组进行了分析,以此为基础设计出11对叶绿体SSR引物,并进一步研究了11对叶绿体SSR引物在铁线莲属其他野生种类和栽培品种中的通用性。结果表明,C. fusca var. coreana叶绿体基因组内共有67个SSR位点,主要分布于1 088~41 445 bp和50 135~127 217 bp,以A/T单碱基重复为主;设计所得11对cpSSR引物在铁线莲野生种类和栽培品种中均表现出良好的通用性,在55个铁线莲样本中扩增等位基因数(Na)平均为10.91,有效等位基因数(Ne)平均为5.40,主等位基因频率(MAF)平均为0.31,多态信息含量(PIC)平均为0.79。另外,基于cpSSR数据,对55个样本的UPGMA聚类结果进行了初步讨论。

关键词: 铁线莲属, 叶绿体微卫星, 引物开发, 等位基因

Abstract: The whole chloroplast genome sequence of Clematis fusca var. coreana from GeneBank database was analyzed, and 11 cpSSR primer pairs were designed based on that. In addition, the transferability of the 11 pairs of cpSSR primer in different species and cultivars of Clematis were studied. The results showed that 67 cpSSRs were distributed across the whole chloroplast genome, and most of them located in the area 1 088-41 445 bp and 50 135-127 217 bp, which generally consisted of a single base A/T. All of the 11 pairs of cpSSR primer could be successfully cross-amplified in different species and cultivars of Clematis, which indicated that the 11 primer pairs for cpSSR markers had good transferability in Clematis. The average number of alleles (Na) was 10.91, effective number of alleles (Ne) was 5.40, major allele frequency (MAF) was 0.31, and polymorphism information content (PIC) was 0.79. In addition, clustering result of UPGMA of 55 samples based on cpSSR data was discussed.

Key words: Clematis, cpSSR, primer development, allele

中图分类号: