杨梅污染肺炎克雷伯氏菌的分离及其耐药特征和毒力基因

doi:10.3969/j.issn.1004-1524.2018.09.10

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (9): 1513-1518.DOI: 10.3969/j.issn.1004-1524.2018.09.10

杨梅污染肺炎克雷伯氏菌的分离及其耐药特征和毒力基因

戴宝玲^{1, 2}, 杨华², 戴贤君¹, 杨桂玲², 汪雯², 肖英平^{2, *}

1.中国计量大学生命科学学院,浙江杭州 310018;
2.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021

收稿日期:2017-09-27 出版日期:2018-09-25 发布日期:2018-10-15
作者简介:戴宝玲(1992—),女,河南信阳人,硕士研究生,研究方向为食品微生物。E-mail: bldaihz123@126.com

Isolation, drug resistance of Klebsiella pneumoniae from waxberry and its virulence gene analysis

DAI Baoling^{1, 2}, YANG Hua², DAI Xianjun¹, YANG Guiling², WANG Wen², XIAO Yingping^{2, *}

1. College of Life Science, China Jiliang University, Hangzhou 310018, China;
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-09-27 Online:2018-09-25 Published:2018-10-15
Contact: 肖英平,E-mail: ypxiaozju@126.com
Supported by:
浙江省植物有害生物防控省部共建国家重点实验室培育基地项目(2010DS700124-ZM1608)

摘要/Abstract

摘要： 杨梅在生产与运输过程中易受到病原菌的污染,为分析杨梅中肺炎克雷伯氏菌的携带情况及肺炎克雷伯氏菌的耐药特征和毒力基因,分别从杭州市场和杨梅生产基地采集90份杨梅样品,测定大肠菌群污染状况,使用梅里埃VITEK 2 Compact全自动微生物鉴定仪和16S rRNA测序方法鉴定肺炎克雷伯氏菌,采用VITEK 2阴性菌药敏卡对所获得的肺炎克雷伯氏菌进行耐药表型分析,PCR扩增检测耐药基因和毒力基因。结果表明:90份样品中共有51份样品存在大肠菌群污染,污染率为56.7%,有9株肺炎克雷伯氏菌,检出率为10%,其中8株肺炎克雷伯氏菌聚为一簇。9株肺炎克雷伯氏菌对氨苄西林、头孢唑林和呋喃咀啶均呈不同程度的耐药,尤其是氨苄西林耐药率达88.89%。9株菌株均携带tetA基因,检出blaTEM、gyrA、aadA1和aac(6')-1b耐药基因的阳性率均为88.89%,blaCTX-M、floR、ereA、ermB和mecA等耐药基因未检出,毒力基因wcaG、magA、rmpA和菌素未检出。综上,杨梅中存在一定的肺炎克雷伯氏菌污染,但这些菌株未携带wcaG、magA、rmpA等毒力基因和菌素。

关键词: 杨梅, 肺炎克雷伯菌, 耐药性, 毒力

Abstract: Waxberry is easy to be polluted by pathogenic bacteria in the process of production and transportation, the main purpose, therefore, is to analyze the carrying status, drug resistance characteristics and virulence genes of Klebsiella pneumoniae in waxberry. 90 samples were collected from Hangzhou market and base. To determine coliform contamination and Klebsiella pneumoniae, the bacteria isolated from the samples were identified by Vitek Auto Microbic System and 16S rRNA sequence. The resistant phenotype of Klebsiella pneumoniae was analyzed by VITEK 2 negative bacteria susceptibility card. Drug resistance genes and virulence genes were detected by PCR amplification. The results showed as follows: 51 samples were contaminated by coliforms in the 90 samples, and the pollution rate was 56.7%; 9 strains of Klebsiella pneumonia were detected, the positive rate was 10%, of which 8 strains were clustered into a cluster with high homology, and the homology of another was significantly different. These 9 strains of Klebsiella pneumonia showed different resistance to ampicillin, cefazolin and nitrofurantain. The resistant rate to ampicillin reached 88.89%. tetA gene was detected in all the 9 strains, and positive rate of drug resistance gene blaTEM, gyrA, aadA1 and AAC(6')-1b were 88.89%, while blaCTX-M, floR, ereA, ermB and mecA gene were not detected. The virulence genes of wcaG, magA, rmpA and Aerobactin were not detected. Qualitative analysis showed that waxberry had a certain contamination of Klebsiella pneumoniae, but these strains did not carry virulence genes such as wcaG, magA, rmpA and Aerobactin.

Key words: waxberry, Klebsiella pneumoniae, drug resistant, virulence

中图分类号:

S662.4

戴宝玲, 杨华, 戴贤君, 杨桂玲, 汪雯, 肖英平. 杨梅污染肺炎克雷伯氏菌的分离及其耐药特征和毒力基因[J]. 浙江农业学报, 2018, 30(9): 1513-1518.

DAI Baoling, YANG Hua, DAI Xianjun, YANG Guiling, WANG Wen, XIAO Yingping. Isolation, drug resistance of Klebsiella pneumoniae from waxberry and its virulence gene analysis[J]. , 2018, 30(9): 1513-1518.

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杨梅污染肺炎克雷伯氏菌的分离及其耐药特征和毒力基因

Isolation, drug resistance of Klebsiella pneumoniae from waxberry and its virulence gene analysis

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