牛抵抗素对牛肺泡巨噬细胞TLR4/MyD88非依赖信号通路基因表达的影响

doi:10.3969/j.issn.1004-1524.2019.03.05

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (3): 379-383.DOI: 10.3969/j.issn.1004-1524.2019.03.05

牛抵抗素对牛肺泡巨噬细胞TLR4/MyD88非依赖信号通路基因表达的影响

阳明贤, 左之才^*, 李碧, 王宇

四川农业大学动物医学院,环境公害与动物疾病四川省高校重点实验室,动物疫病与人类健康四川省重点实验室,四川成都 611130

收稿日期:2018-06-12 出版日期:2019-03-25 发布日期:2019-04-08
通讯作者: *左之才,E-mail: zzcjl@126.com
作者简介:阳明贤(1993—),男,重庆梁平人,硕士,研究方向为中西兽医与临床。E-mail: 2812188700@qq.com
基金资助:
国家重点研发计划(2018YFD0501800); 四川省科技计划(2018NZ002); 国家现代农业产业技术体系四川省创新团队(035Z2036)

Analysis of differential expression of TLR4-MyD88 independent pathway genes under resistin-induced bovine alveolar macrophages

YANG Mingxian, ZUO Zhicai^*, LI Bi, WANG Yu

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Key Laboratory of Environmental Hazard and Animal Disease of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2018-06-12 Online:2019-03-25 Published:2019-04-08

摘要/Abstract

摘要： 旨在探究牛抵抗素通过TLR4/MyD88非依赖信号通路诱发牛肺泡巨噬细胞的相关炎症反应机理。使用100 ng·mL^-1终浓度牛抵抗素诱导牛肺泡巨噬细胞,分别在0、1.5、3.0、6.0、12.0、24.0 h时采用qRT-PCR检测TLR4/MyD88非依赖信号通路相关基因(TLR4、TRAM、NF-κB)mRNA水平表达量,通过ELISA法检测下游IL-6、1L-1β、TNF-α等促炎细胞因子表达水平。结果显示,100 ng·mL^-1牛抵抗素处理牛肺泡巨噬细胞后,TLR4、TRAM、NF-κB基因表达量于6.0 h时极显著上调(P<0.01),诱导12.0 h时,相关基因表达量达到最高;IL-6、1L-1β、TNF-α等促炎细胞因子于1.5 h表达量极显著上调(P<0.01),且均存在时间依赖效应。抵抗素诱导牛肺泡巨噬细胞后,可激活TLR4/MyD88非依赖信号通路,通过信号传递,释放大量促炎细胞因子,引起肺部炎症。

关键词: 牛抵抗素, 牛肺泡巨噬细胞, TLR4/MyD88非依赖信号通路, 促炎细胞因子

Abstract: In order to explore the mechanism of bovine resistin through TLR4-MyD88 independent signal pathway in bovine alveolar macrophages, bovine alveolar macrophages were exposed to resistin(100 ng·mL^-1) for 0, 1.5, 3.0, 6.0, 12.0, 24.0 h, respectively. The relative mRNA expression of TLR4-MyD88 independent signal pathway genes (TLR4, TRAM, NF-κB) were detected by qRT-PCR, the expression of proinflammatory cytokine IL-6, 1L-1β and TNF-α were tested by ELISA methods. The results showed that resistin(100 ng·mL^-1) sharply upregulated the expression of TLR4-MyD88 independent signal pathway genes at 6.0 h(P<0.01). The expression of related genes reached the highest at 12.0 h. The expression of proinflammatory cytokines IL-6, IL-1β and TNF-α significantly increased at 1.5 h (P<0.01). Through the signal transmission, a large number of proinflammatory cytokines were released, causing pulmonary inflammation.

Key words: resistin, bovine alveolar macrophages, TLR4-MyD88 inpendent signal pathway, proinflammatory cytokine

中图分类号:

S852
R563.1

阳明贤, 左之才, 李碧, 王宇. 牛抵抗素对牛肺泡巨噬细胞TLR4/MyD88非依赖信号通路基因表达的影响[J]. 浙江农业学报, 2019, 31(3): 379-383.

YANG Mingxian, ZUO Zhicai, LI Bi, WANG Yu. Analysis of differential expression of TLR4-MyD88 independent pathway genes under resistin-induced bovine alveolar macrophages[J]. , 2019, 31(3): 379-383.

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牛抵抗素对牛肺泡巨噬细胞TLR4/MyD88非依赖信号通路基因表达的影响

Analysis of differential expression of TLR4-MyD88 independent pathway genes under resistin-induced bovine alveolar macrophages

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