大花蕙兰花粉贮藏与离体萌发研究

doi:10.3969/j.issn.1004-1524.2019.09.14

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (9): 1502-1508.DOI: 10.3969/j.issn.1004-1524.2019.09.14

大花蕙兰花粉贮藏与离体萌发研究

张晓莹, 傅巧娟^*, 赵福康, 李春楠, 阮若昕

杭州市农业科学研究院园艺研究所,浙江杭州 310024

收稿日期:2019-04-25 出版日期:2019-09-25 发布日期:2019-10-11
通讯作者: *傅巧娟,E-mail: fqjwxj@126.com
作者简介:张晓莹(1986—),女,黑龙江伊春人,博士,助理研究员,研究方向为观赏植物遗传育种。E-mail: zxiaoying2013@163.com
基金资助:
杭州市农业与社会发展主动设计项目(20172015A04)

Study on preservation and germination of Cymbidium hybridum pollen

ZHANG Xiaoying, FU Qiaojuan^*, ZHAO Fukang, LI Chunnan, RUAN Ruoxin

Institute of Horticulture, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2019-04-25 Online:2019-09-25 Published:2019-10-11

摘要/Abstract

摘要： 为解决兰花花期不遇的问题,为杂交育种工作提供参考与借鉴。以大花蕙兰(Cymbidium hybridum)品种JOIC和KK560为试材,筛选适宜的花粉离体萌发培养基配方;检测不同贮藏温度、不同保存时间的大花蕙兰花粉离体萌发率,确定适宜的花粉保存方法。结果显示,大花蕙兰品种JOIC花粉粒离体萌发的最适培养基配方为0.5 MS+30%蔗糖,大花蕙兰品种KK560花粉粒离体萌发的最适培养基配方为0.01%硼酸+0.2%氯化钙+30%蔗糖。建议保存前将花粉块硅胶阴干24 h,-20 ℃保存,期限为6个月。

关键词: 大花蕙兰, 花粉贮藏, 花粉活力, 离体萌发

Abstract: To solve the problem of the flowering worst of orchid and provide reference for orchid hybridization breeding. This study have used the Cymbidium hybridum species JOIC and KK560 as test materials to screen the optimal medium for in vitro pollen germination, and detected the in vitro germination rate of Cymbidium hybridum pollen with different storage temperature and storage time to determine the appropriate pollen preservation method. The results showed that the optimum medium for in vitro germination of JOIC pollen was 0.5 MS+30% sucrose, and the optimum medium for in vitro germination of KK560 pollen was 0.01% H₃BO₃+0.2% CaCl₂+30% sucrose. Pollinium should be dried in the shade with silicone for 24 h before preservation and be preserved in -20 ℃ for six months.

Key words: Cymbidium hybridum, pollen preservation, pollen viability, in vitro germination

中图分类号:

S682.31

张晓莹, 傅巧娟, 赵福康, 李春楠, 阮若昕. 大花蕙兰花粉贮藏与离体萌发研究[J]. 浙江农业学报, 2019, 31(9): 1502-1508.

ZHANG Xiaoying, FU Qiaojuan, ZHAO Fukang, LI Chunnan, RUAN Ruoxin. Study on preservation and germination of Cymbidium hybridum pollen[J]. , 2019, 31(9): 1502-1508.

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大花蕙兰花粉贮藏与离体萌发研究

Study on preservation and germination of Cymbidium hybridum pollen

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