猪FTO基因多态性与肉质性状的关联分析

doi:10.3969/j.issn.1004-1524.2019.12.04

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (12): 1971-1978.DOI: 10.3969/j.issn.1004-1524.2019.12.04

猪FTO基因多态性与肉质性状的关联分析

杨又兵^1,2, 卞军平^1,2, 吴燕^1,2, 娄然^1,2, 李世豪^1,2

1.河南科技大学动物科技学院,河南洛阳 471023;
2.洛阳市动物遗传育种重点实验室,河南洛阳 471023

收稿日期:2019-06-09 出版日期:2019-12-25 发布日期:2019-12-25
作者简介:杨又兵(1977-),男,湖北黄冈人,博士,副教授,研究方向为动物遗传育种与繁殖。E-mail:yangyoubing@sina.com
基金资助:
国家自然科学基金委员会-河南省人民政府人才培养联合基金(U1304324)

Association analysis between polymorphism in FTO gene and meat quality traits in pigs

YANG Youbing^1,2, BIAN Junping^1,2, WU Yan^1,2, LOU Ran^1,2, LI Shihao^1,2

1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China;
2. Key Laboratory of Animal Genetics and Breeding of Luoyang City, Luoyang 471023, China

Received:2019-06-09 Online:2019-12-25 Published:2019-12-25

摘要/Abstract

摘要： 为了探究猪FTO基因多态性与肉质性状的关联性,试验通过限制性片段长度多态性聚合酶链式反应(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)的BstuⅠ和TaiⅠ限制性内切酶技术分析了豫西黑猪和长白猪FTO基因第3外显子(c.594C>G)和第4内含子(g.276G>T)的遗传多样性,并将FTO基因的基因型与豫西黑猪背膘厚、pH₁、pH₂₄、滴水损失、肉色和大理石纹等肉质性状进行了关联分析。研究结果表明:FTO基因的c.594C>G位点和g.276G>T位点都具有中度多态性,经检验c.594C>G位点和g.276G>T位点的基因频率均符合χ²检验的Hardy-Weinberg平衡定律(P>0.05)。在c.594C>G位点上,豫西黑猪和长白猪都存在3种不同的基因型,分别为CC、CG和GG基因型,优势等位基因都为C等位基因,且两者C等位基因的频率分别为0.522 7和0.533 3,在肉色评分上,CG基因型和GG基因型个体显著高于CC基因型(P<0.05)。在g.276G>T位点上,豫西黑猪和长白猪都存在3种不同的基因型,分别为GG、GT和TT基因型,优势等位基因均为T等位基因(0.574 1,1.648 1)。结果表明,FTO基因的c.594C>G位点与豫西黑猪的肉质性状具有显著关联性,这在一定程度上说明了FTO基因可以为豫西黑猪肉质性状的遗传选择提供参考依据。

Abstract: In order to investigate the association between the FTO polymorphism and meat quality traits in pigs, PCR-RFLPs with BstuⅠ and TaiⅠ restriction enzymes were used to analyze genetic diversities of two SNPs at the third exon (c.594C>G) and the fourth intron (g.276G>T) of the FTO gene in Henan western black pigs and Landrace pigs, potential associations between genotypes and six meat quality traits including back fat thickness, pH₁, pH₂₄, drip loss, color score and muscle marbling were investigated. The results showed as follows: The population of Henan western black pigs had medium level of internal genetic variation. Two SNPs (c.594C>G and g.276G>T) of FTO gene had moderate polymorphism. For c.594C>G SNP, the results showed that there were 3 different genotypes (CC, CG and GG) in Henan western black pigs and Landrace pigs. C allele was dominant with the frequency of Henan western black pigs and Landrace pigs of 0.522 7, 0.533 3; the individuals with CG and GG genotype had significantly higher color score than individuals with CC (P<0.05). For g.276G>T SNP, the results showed that there were 3 different genotypes (GG, GT and TT) in Henan western black pigs and Landrace pigs. The T allele was dominant with the frequency of Henan western black pigs and Landrace pigs being 0.574 1, 0.648 1. At the c.594C>G locus, allele G significantly increased meat color, and CG genotype individuals and GG genotype individuals were significantly higher than CC genotype individuals (P<0.05). At the g.276G>T locus, the allele G could increase the backfat thickness, pH₁ and pH₂₄ values, and reduce the drip loss. The results demonstrated that the c.594C>G locus of FTO gene was associated with meat quality traits of Henan western black pigs, whether FTO gene would provide a reference for meat quality traits selection in Henan western black pigs offspring.

Key words: Henan western black pig, fat mass and obesity associated gene (FTO), PCR-PFLR, genetic polymorphism, meat quality trait

中图分类号:

S828

杨又兵, 卞军平, 吴燕, 娄然, 李世豪. 猪FTO基因多态性与肉质性状的关联分析[J]. 浙江农业学报, 2019, 31(12): 1971-1978.

YANG Youbing, BIAN Junping, WU Yan, LOU Ran, LI Shihao. Association analysis between polymorphism in FTO gene and meat quality traits in pigs[J]. , 2019, 31(12): 1971-1978.

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猪FTO基因多态性与肉质性状的关联分析

Association analysis between polymorphism in FTO gene and meat quality traits in pigs

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