不同生长时期红螯螯虾表型性状差异分析

doi:10.3969/j.issn.1004-1524.2020.12.06

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (12): 2154-2161.DOI: 10.3969/j.issn.1004-1524.2020.12.06

不同生长时期红螯螯虾表型性状差异分析

陈红林¹(), 秦高婵², 楼宝¹^,^*(), 钱豪杰³, 姚振海⁴

1.浙江省农业科学院水生生物研究所,浙江杭州 310021
2.浙江海洋大学水产学院,浙江舟山 316022
3.海宁市鸿海养殖有限公司,浙江海宁 314400
4.海宁市水产技术服务站,浙江海宁 314400

收稿日期:2020-06-16 出版日期:2020-12-25 发布日期:2020-12-25
通讯作者: 楼宝
作者简介:*楼宝,E-mail: loubao6577@163.com
陈红林(1989—),女,辽宁铁岭人,博士,助理研究员,主要从事水产动物遗传育种研究。E-mail: chenhonglin@zaas.ac.cn
基金资助:
浙江省农业科学院水产学科建设经费(SSS2019005)

Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages

CHEN Honglin¹(), QIN Gaochan², LOU Bao¹^,^*(), QIAN Haojie³, YAO Zhenhai⁴

1. Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. School of Fishery, Zhejiang Ocean University, Zhoushan 316022, China
3. Honghai Culture Co., Ltd., Haining 314400, China
4. Haining Aquaculture Technology Service Station of Zhejiang Province, Haining 314400, China

Received:2020-06-16 Online:2020-12-25 Published:2020-12-25
Contact: LOU Bao

摘要/Abstract

摘要：

为研究红螯螯虾(Cherax quadricarinatus von Martens)不同生长时期的表型特征,统计了3月龄、4.5月龄和6月龄3个生长时期红螯螯虾的体重Y₁、肝胰腺质量Y₂、全长X₁、头胸甲长X₂、头胸甲宽X₃、腹节总长X₄、第一腹节长X₅、第一腹节宽X₆和掌节长X₇共9个性状,对比3个生长时期各表型性状间的差异和相关性,分析不同生长时期各表型性状在雌性与雄性群体间的差异,采用多元回归分析分别研究了不同生长时期表型性状与体重的关系,并构建回归方程。结果显示,头胸甲和腹节的长度比例在幼虾和成虾中保持一致,而宽度指标(头胸甲宽、第一腹节宽)在6月龄阶段显著(P<0.05)增长。随着红螯螯虾的生长,大部分性状间的相关性降低,雌雄群体间表型性状的差异逐渐增加。3月龄阶段的回归方程为Y₁=-31.33+0.73X₂+0.91X₃;4.5月龄阶段的回归方程分别为Y₁=-65.25+1.29X₃+0.89X₄+1.46X₆(雌性)、Y₁=-69.67+1.17X₃+0.40X₇+1.77X₆+0.59X₄(雄性);6月龄阶段的回归方程分别为Y₁=-79.77+1.01X₁-1.89X₆+1.15X₇(雌性)、Y₁=-98.02+0.72X₁+1.22X₇(雄性)。从3个生长时期的回归方程可以看出,各表型性状在不同时期与体重的相关性差异较大。研究结果可为红螯螯虾的选育工作提供理论依据。

关键词: 红螯螯虾, 表型性状, 雌雄差异, 相关系数, 回归分析

Abstract:

In order to study the phenotypic characteristics of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages, nine phenotypic traits including body weight (Y₁), hepatopancreas weight (Y₂), full-length (X₁), cephalothorax length (X₂), cephalothorax width (X₃), total abdominal segment length (X₄), first abdominal segment length (X₅), first abdominal segment width (X₆), propodite length (X₇) in three growth stages of 3 months, 4.5 months and 6 months were measured and analyzed. The differences of the phenotypic traits in the three stages were compared, and the correlations of the phenotypic traits in different growth stages were analyzed. Multiple regression equations of phenotypic traits to body weight of male and female red claw crayfish populations in different growth stages were constructed to measure the relevance of phenotypic traits and body weight. The results showed that the proportion of cephalothorax or abdominal segments to full-length remained the same in juvenile and adult stage, while the width index increased significantly (P<0.05) at 6 months of age. With the growth of red claw crayfish, the correlations among the majority of the traits decreased, and the differences in phenotypic characteristics between male and female populations gradually increased. The regression equation in 3 months of age was Y₁=-31.33+0.73X₂+0.91X₃. The regression equation in 4.5 months of age was Y₁=-65.25+1.29X₃+0.89X₄+1.46X₆ for female and Y₁=-69.67+1.17X₃+0.40X₇+1.77X₆+0.59X₄ for male, respectively. The regression equation in 6 months of age was Y₁=-79.77+1.01X₁-1.89X₆+1.15X₇ for female and Y₁=-98.02+0.72X₁+1.22X₇ for male, respectively. These results could provide theoretical basis for the selective breeding of red claw crayfish.

Key words: red claw crayfish, phenotypic characters, sexual dimorphism, correlation coefficient, regression analysis

中图分类号:

S966.12

陈红林, 秦高婵, 楼宝, 钱豪杰, 姚振海. 不同生长时期红螯螯虾表型性状差异分析[J]. 浙江农业学报, 2020, 32(12): 2154-2161.

CHEN Honglin, QIN Gaochan, LOU Bao, QIAN Haojie, YAO Zhenhai. Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2154-2161.

图/表 6

参考文献 25

[1]	MAC LOUGHLIN C, CANOSA I S, SILVEYRA G R, et al. Effects of atrazine on growth and sex differentiation, in juveniles of the freshwater crayfish Cherax quadricarinatus[J]. Ecotoxicology and Environmental Safety, 2016,131:96-103. URL PMID
[2]	ROUSE D B, AUSTIN C M, MEDLEY P B. Progress toward profits?: information on the Australian crayfish[J]. Aquaculture Magazine, 1991,17(3):46-56.
[3]	WICKINS J F, O’C LEE D. Crustacean farming, ranching and culture[J]. Aquaculture Research, 2003,34(3):269-270. DOI URL
[4]	TIERNEY L J, WILD C H, FURSE J M. Total incombustible (mineral) content of Cherax quadricarinatus differs between feral populations in Central-Eastern Australia[J]. PeerJ, 2019,7:e6351. URL PMID
[5]	王广军, 孙悦, 郁二蒙, 等. 澳洲淡水龙虾与克氏原螯虾肌肉营养成分分析与品质评价[J]. 动物营养学报, 2019,31(9):4339-4348.
	WANG G J, SUN Y, YU E M, et al. Analysis and quality evaluation of nutrient components in muscle of Cherax quadricarinatus and Procambarus clarkii[J]. Chinese Journal of Animal Nutrition, 2019,31(9):4339-4348.(in Chinese with English abstract)
[6]	顾志敏, 许谷星, 黄鲜明, 等. 红螯螯虾的室内人工育苗[J]. 水产学报, 2003,27(1):32-37.
	GU Z M, XU G X, HUANG X M, et al. Indoor artificial breeding and juvenile nursing of Cherax quadricarinatus[J]. Journal of Fisheries of China, 2003,27(1):32-37.(in Chinese with English abstract)
[7]	CORTÉS-JACINTO E, VILLARREAL-COLMENARES H, CIVERA-CERECEDO R, et al. Effect of dietary protein level on growth and survival of juvenile freshwater crayfish Cherax quadricarinatus(Decapoda: Parastacidae)[J]. Aquaculture Nutrition, 2003,9(4):207-213. DOI URL
[8]	GAO M L, LI F, XU L M, et al. White spot syndrome virus strains of different virulence induce distinct immune response in Cherax quadricarinatus[J]. Fish & Shellfish Immunology, 2014,39(1):17-23. DOI URL PMID
[9]	LIU H P, CHEN R Y, ZHANG Q X, et al. Differential gene expression profile from haematopoietic tissue stem cells of red claw crayfish, Cherax quadricarinatus, in response to WSSV infection[J]. Developmental and Comparative Immunology, 2011,35(7):716-724. DOI URL PMID
[10]	WANG D L, ZUO D, WANG L M, et al. Effects of white spot syndrome virus infection on immuno-enzyme activities and ultrastructure in gills of Cherax quadricarinatus[J]. Fish & Shellfish Immunology, 2012,32(5):645-650. DOI URL PMID
[11]	DUAN H, JIN S J, ZHANG Y, et al. Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways[J]. Developmental & Comparative Immunology, 2014,46(2):186-193. DOI URL PMID
[12]	BRYLAWSKI B J, MILLER T J. Temperature-dependent growth of the blue crab (Callinectes sapidus): a molt process approach[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2006,63(6):1298-1308. DOI URL
[13]	MANOR R, AFLALO E D, SEGALL C, et al. Androgenic gland implantation promotes growth and inhibits vitellogenesis in Cherax quadricarinatus females held in individual compartments[J]. Invertebrate Reproduction & Development, 2004,45(2):151-159.
[14]	JONES C M. Production of juvenile redclaw crayfish, Cherax quadricarinatus(Von Martens) (Decapoda, Parastacidae) I: development of hatchery and nursery procedures[J]. Aquaculture, 1995,138(1/2/3/4):221-238. DOI URL
[15]	CURTIS M C, JONES C M. Observations on monosex culture of redclaw crayfish Cherax quadricarinatus von Martens (Decapoda: Parastacidae) in earthen ponds[J]. Journal of the World Aquaculture Society, 1995,26(2):154-159. DOI URL
[16]	费志良, 宋胜磊, 唐建清, 等. 克氏原螯虾含肉率及蜕皮周期中微量元素分析[J]. 水产科学, 2005,24(10):8-11.
	FEI Z L, SONG S L, TANG J Q, et al. Dressed crayfish and microelement levels in Procambarus clarkii during ecdysis[J]. Fisheries Science, 2005,24(10):8-11.(in Chinese with English abstract)
[17]	NISKANEN H, MANNONEN A, JUSSILA J. Variation in pleonite width in wild noble crayfish, Astacus astacus, females in relation to glair gland development[J]. Freshwater Crayfish, 1996 (11):378-383.
[18]	HOLLAND D L. Lipid reserves and energy metabolism in the larvae of benthic marine invertebrates[M]//SARGENT D C, MALINS J R. Biochemical and biophysical perspectives in marine biology. London: Academic Press, 1978: 85-123.
[19]	COCCIA E, DE LISA E, DI CRISTO C, et al. Effects of estradiol and progesterone on the reproduction of the freshwater crayfish Cherax albidus[J]. The Biological Bulletin, 2010,218(1):36-47. URL PMID
[20]	VOGT G. Functional cytology of the hepatopancreas of decapod crustaceans[J]. Journal of Morphology, 2019,280(9):1405-1444. URL PMID
[21]	RODRÍGUEZ-GONZÁLEZ H, HERNÁNDEZ-LLAMAS A, VILLARREAL H, et al. Gonadal development and biochemical composition of female crayfish Cherax quadricarinatus(Decapoda: Parastacidae) in relation to the gonadosomatic index at first maturation[J]. Aquaculture, 2006,254(1/2/3/4):637-645. DOI URL
[22]	RODRÍGUEZ-GONZÁLEZ H, VILLARREAL H, GARCÍA-ULLOA M, et al. Dietary lipid requirements for optimal egg quality of redclaw crayfish, Cherax quadricarinatus[J]. Journal of the World Aquaculture Society, 2009,40(4):531-539. DOI URL
[23]	SAGI A, KHALAILA I, ABDU U, et al. A newly established ELISA showing the effect of the androgenic gland on secondary-vitellogenic-specific protein in the hemolymph of the crayfish Cherax quadricarinatus[J]. General and Comparative Endocrinology, 1999,115(1):37-45. URL PMID
[24]	ABDU U, YEHEZKEL G, SAGI A. Oocyte development and polypeptide dynamics during ovarian maturation in the red-claw crayfish Cherax quadricarinatus[J]. Invertebrate Reproduction & Development, 2000,37(1):75-83.
[25]	TSENG D Y, CHEN Y N, KOU G H, et al. Hepatopancreas is the extraovarian site of vitellogenin synjournal in black tiger shrimp, Penaeus monodon[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2001,129(4):909-917. DOI URL

性状 Trait	月龄 Month	平均值 Mean	变异系数 CV/%
Y₁/g	3	19.30	28.03
	4.5	40.86	21.39
	6	62.75	24.57
Y₂/g	3	1.32	0.29
	4.5	2.75	0.26
	6	4.30	0.24
X₁/mm	3	94.44	10.11
	4.5	122.65	8.10
	6	138.72	7.76
X₂/mm	3	45.67	9.81
	4.5	59.17	8.01
	6	67.66	8.20
X₃/mm	3	18.93	12.04
	4.5	24.57	8.22
	6	29.10	8.52
X₄/mm	3	32.93	9.75
	4.5	42.57	7.56
	6	48.14	7.19
X₅/mm	3	7.62	10.37
	4.5	9.79	8.07
	6	11.72	14.51
X₆/mm	3	18.13	9.93
	4.5	23.97	9.01
	6	27.99	9.90
X₇/mm	3	28.44	11.60
	4.5	39.32	12.03
	6	50.35	12.95

性状 Trait	月龄 Month	平均值 Mean	变异系数 CV/%
Y₁/g	3	19.30	28.03
	4.5	40.86	21.39
	6	62.75	24.57
Y₂/g	3	1.32	0.29
	4.5	2.75	0.26
	6	4.30	0.24
X₁/mm	3	94.44	10.11
	4.5	122.65	8.10
	6	138.72	7.76
X₂/mm	3	45.67	9.81
	4.5	59.17	8.01
	6	67.66	8.20
X₃/mm	3	18.93	12.04
	4.5	24.57	8.22
	6	29.10	8.52
X₄/mm	3	32.93	9.75
	4.5	42.57	7.56
	6	48.14	7.19
X₅/mm	3	7.62	10.37
	4.5	9.79	8.07
	6	11.72	14.51
X₆/mm	3	18.13	9.93
	4.5	23.97	9.01
	6	27.99	9.90
X₇/mm	3	28.44	11.60
	4.5	39.32	12.03
	6	50.35	12.95

生长时期	X₂/X₁	X₃/X₁	X₄/X₁	X₅/X₁	X₆/X₁	X₇/X₁	Y₂/Y₁
Growth stage
3月龄3 months	48.89±0.97 a	20.31±0.73 b	34.33±0.99 a	7.99±0.50 b	18.84±0.63 b	31.26±2.27 c	7.03±1.05 a
4.5月龄4.5 months	48.28±1.11 a	20.05±0.65 b	34.72±1.08 a	7.99±0.48 b	19.54±0.74 b	32.07±2.76 b	6.79±1.29 a
6月龄 6 months	48.77±1.54 a	21.00±1.20 a	34.74±1.30 a	8.47±1.21 a	20.20±1.55 a	36.21±2.80 a	6.98±1.20 a

生长时期	X₂/X₁	X₃/X₁	X₄/X₁	X₅/X₁	X₆/X₁	X₇/X₁	Y₂/Y₁
Growth stage
3月龄3 months	48.89±0.97 a	20.31±0.73 b	34.33±0.99 a	7.99±0.50 b	18.84±0.63 b	31.26±2.27 c	7.03±1.05 a
4.5月龄4.5 months	48.28±1.11 a	20.05±0.65 b	34.72±1.08 a	7.99±0.48 b	19.54±0.74 b	32.07±2.76 b	6.79±1.29 a
6月龄 6 months	48.77±1.54 a	21.00±1.20 a	34.74±1.30 a	8.47±1.21 a	20.20±1.55 a	36.21±2.80 a	6.98±1.20 a

性状Trait	性别Sex	3月龄3 months	4.5月龄4.5 months	6月龄6 months
Y₁	雌Female	24.44±7.07	41.04±8.34	57.93±17.24^*
	雄Male	24.77±6.70	40.44±9.31	65.45±13.92
Y₂	雌Female	1.72±0.61	2.96±0.72^**	4.13±0.99
	雄Male	1.74±0.50	2.53±0.65	4.4±1.03
X₁	雌Female	103.02±11.47	123.99±9.44	137.94±14.09
	雄Male	103.05±10.63	121.34±10.41	139.42±8.41
X₂	雌Female	50.24±5.82	59.48±4.66	66.81±6.30
	雄Male	50.75±5.78	58.79±4.90	68.42±4.62
X₃	雌Female	20.87±2.62	24.92±1.94	29.25±3.21
	雄Male	21.17±2.67	24.19±2.10	29.04±2.03
X₄	雌Female	35.44±3.53	42.94±3.04	49.05±4.43
	雄Male	35.03±3.33	42.06±3.39	47.66±2.74
X₅	雌Female	8.21±1.00	9.81±0.81	12.66±2.37^**
	雄Male	8.24±0.88	9.73±0.79	11.19±0.86
X₆	雌Female	19.49±2.02	24.65±2.20^**	29.96±3.40^**
	雄Male	19.07±1.74	23.23±1.93	26.92±1.57
X₇	雌Female	31.92±4.60	38.58±4.74	48.16±6.92^*
	雄Male	32.95±4.94	40.07±4.78	51.60±6.08

不同生长时期红螯螯虾表型性状差异分析

Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 25

相关文章 15

编辑推荐

Metrics

本文评价

性状Trait	Y₁	Y₂	X₁	X₂	X₃	X₄	X₅	X₆	X₇
Y₁	1.00	0.81	0.98^**	0.98^**	0.98^**	0.97^**	0.84	0.97^**	0.54
Y₂	0.79	1.00	0.79	0.79	0.82	0.72	0.63	0.77	0.47
X₁	0.96^**	0.70	1.00	0.98^**	0.95^**	0.97^**	0.84	0.97^**	0.46
X₂	0.95^**	0.68	0.97^**	1.00	0.96^**	0.97^**	0.84	0.98^**	0.49
X₃	0.94^**	0.70	0.94^**	0.94^**	1.00	0.94^**	0.81	0.95^**	0.52
X₄	0.96^**	0.79	0.94^**	0.92^**	0.91^**	1.00	0.87^**	0.98^**	0.44
X₅	0.82	0.71	0.81	0.82	0.78	0.86^**	1.00	0.87^**	0.39
X₆	0.94^**	0.80	0.94^**	0.91^**	0.90^**	0.95^**	0.84	1.00	0.44
X₇	0.77	0.79	0.81	0.76	0.70	0.68	0.70	0.52	1.00

[1]	洪霞, 赵永彬, 屈为栋, 陈银龙, 邱莉萍, 王娇阳. 基于表型性状与简单重复序列标记的浙江省芋种质资源遗传多样性比较[J]. 浙江农业学报, 2020, 32(9): 1544-1554.
[2]	楼宇杰, 张本效, 王真真. 村级集体经济经营性收入影响因素分析——基于浙江省金华市的调查数据[J]. 浙江农业学报, 2020, 32(8): 1506-1512.
[3]	谢文钢, 李晓松, 李伟, 唐茜. 四川地方中小叶茶树资源的表型遗传多样性[J]. 浙江农业学报, 2019, 31(9): 1405-1415.
[4]	刘志, 贺正, 苗芳芳, 贾彪. 基于无人机的水肥一体化玉米出苗率估算方法与试验[J]. 浙江农业学报, 2019, 31(6): 977-985.
[5]	陈越, 张敦宇, 丁明亮, 王玲仙, 肖素勤, 柯学, 程在全. 多个省份水稻资源的表型多样性与优异资源的筛选[J]. 浙江农业学报, 2019, 31(11): 1779-1789.
[6]	胡志辉, 汪艳杰, 张丽琴. 菜用大豆施肥后荧光、光谱、光合等参数对产量的预测[J]. 浙江农业学报, 2018, 30(8): 1355-1362.
[7]	白羽祥, 杨焕文, 徐照丽, 吴涛, 易建华, 王戈. 连作植烟土壤中酚酸物质与土壤因子的关系分析[J]. 浙江农业学报, 2018, 30(11): 1907-1914.
[8]	陈春玲, 马航, 许童羽, 周云成, 于丰华, 余昌乐. 东北粳稻叶片植被指数NDVI与PRI的相关性分析[J]. 浙江农业学报, 2016, 28(12): 1963-1969.
[9]	许童羽, 洪雪, 陈春玲, 周云成, 曹英丽, 于丰华, 李娜. 基于冠层NDVI数据的北方粳稻产量模型研究[J]. 浙江农业学报, 2016, 28(10): 1790-1795.
[10]	王志明1，王婕姝1，王毅2，韩向敏1，*，王华1. 甘肃高山细毛羊体重与体尺指标的相关性研究[J]. 浙江农业学报, 2016, 28(1): 28-.
[11]	张英虎，陈和，陈健，陶红，乔海龙，臧慧，栾海业，沈会权*. 2007—2014年江苏沿海地区大麦品种（系）产量与产量构成分析[J]. 浙江农业学报, 2015, 27(9): 1510-.
[12]	高光照*，陈国胜. 温州本埠与外埠农民工市民化意愿比较分析[J]. 浙江农业学报, 2015, 27(8): 1499-.
[13]	范坤;冯长焕;汤泽生;杨军;彭正松;. 航天诱变凤仙花SP1代单株小孢子大小的回归分析[J]. , 2012, 24(4): 0-596.
[14]	范坤;冯长焕;汤泽生;杨军;彭正松;. 航天诱变凤仙花SP1代单株小孢子大小的回归分析[J]. , 2012, 24(4): 0-596.
[15]	于建军;闫鼎;叶贤文;马云明;卫盼盼;刘茜. 重庆地区烤烟主要化学成分与评吸质量分析[J]. , 2010, 22(5): 669-672.