不同生长时期红螯螯虾表型性状差异分析

doi:10.3969/j.issn.1004-1524.2020.12.06

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (12): 2154-2161.DOI: 10.3969/j.issn.1004-1524.2020.12.06

不同生长时期红螯螯虾表型性状差异分析

陈红林¹(), 秦高婵², 楼宝¹^,^*(), 钱豪杰³, 姚振海⁴

1.浙江省农业科学院水生生物研究所,浙江杭州 310021
2.浙江海洋大学水产学院,浙江舟山 316022
3.海宁市鸿海养殖有限公司,浙江海宁 314400
4.海宁市水产技术服务站,浙江海宁 314400

收稿日期:2020-06-16 出版日期:2020-12-25 发布日期:2020-12-25
通讯作者: 楼宝
作者简介:*楼宝,E-mail: loubao6577@163.com
陈红林(1989—),女,辽宁铁岭人,博士,助理研究员,主要从事水产动物遗传育种研究。E-mail: chenhonglin@zaas.ac.cn
基金资助:
浙江省农业科学院水产学科建设经费(SSS2019005)

Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages

CHEN Honglin¹(), QIN Gaochan², LOU Bao¹^,^*(), QIAN Haojie³, YAO Zhenhai⁴

1. Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. School of Fishery, Zhejiang Ocean University, Zhoushan 316022, China
3. Honghai Culture Co., Ltd., Haining 314400, China
4. Haining Aquaculture Technology Service Station of Zhejiang Province, Haining 314400, China

Received:2020-06-16 Online:2020-12-25 Published:2020-12-25
Contact: LOU Bao

摘要/Abstract

摘要：

为研究红螯螯虾(Cherax quadricarinatus von Martens)不同生长时期的表型特征,统计了3月龄、4.5月龄和6月龄3个生长时期红螯螯虾的体重Y₁、肝胰腺质量Y₂、全长X₁、头胸甲长X₂、头胸甲宽X₃、腹节总长X₄、第一腹节长X₅、第一腹节宽X₆和掌节长X₇共9个性状,对比3个生长时期各表型性状间的差异和相关性,分析不同生长时期各表型性状在雌性与雄性群体间的差异,采用多元回归分析分别研究了不同生长时期表型性状与体重的关系,并构建回归方程。结果显示,头胸甲和腹节的长度比例在幼虾和成虾中保持一致,而宽度指标(头胸甲宽、第一腹节宽)在6月龄阶段显著(P<0.05)增长。随着红螯螯虾的生长,大部分性状间的相关性降低,雌雄群体间表型性状的差异逐渐增加。3月龄阶段的回归方程为Y₁=-31.33+0.73X₂+0.91X₃;4.5月龄阶段的回归方程分别为Y₁=-65.25+1.29X₃+0.89X₄+1.46X₆(雌性)、Y₁=-69.67+1.17X₃+0.40X₇+1.77X₆+0.59X₄(雄性);6月龄阶段的回归方程分别为Y₁=-79.77+1.01X₁-1.89X₆+1.15X₇(雌性)、Y₁=-98.02+0.72X₁+1.22X₇(雄性)。从3个生长时期的回归方程可以看出,各表型性状在不同时期与体重的相关性差异较大。研究结果可为红螯螯虾的选育工作提供理论依据。

关键词: 红螯螯虾, 表型性状, 雌雄差异, 相关系数, 回归分析

Abstract:

In order to study the phenotypic characteristics of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages, nine phenotypic traits including body weight (Y₁), hepatopancreas weight (Y₂), full-length (X₁), cephalothorax length (X₂), cephalothorax width (X₃), total abdominal segment length (X₄), first abdominal segment length (X₅), first abdominal segment width (X₆), propodite length (X₇) in three growth stages of 3 months, 4.5 months and 6 months were measured and analyzed. The differences of the phenotypic traits in the three stages were compared, and the correlations of the phenotypic traits in different growth stages were analyzed. Multiple regression equations of phenotypic traits to body weight of male and female red claw crayfish populations in different growth stages were constructed to measure the relevance of phenotypic traits and body weight. The results showed that the proportion of cephalothorax or abdominal segments to full-length remained the same in juvenile and adult stage, while the width index increased significantly (P<0.05) at 6 months of age. With the growth of red claw crayfish, the correlations among the majority of the traits decreased, and the differences in phenotypic characteristics between male and female populations gradually increased. The regression equation in 3 months of age was Y₁=-31.33+0.73X₂+0.91X₃. The regression equation in 4.5 months of age was Y₁=-65.25+1.29X₃+0.89X₄+1.46X₆ for female and Y₁=-69.67+1.17X₃+0.40X₇+1.77X₆+0.59X₄ for male, respectively. The regression equation in 6 months of age was Y₁=-79.77+1.01X₁-1.89X₆+1.15X₇ for female and Y₁=-98.02+0.72X₁+1.22X₇ for male, respectively. These results could provide theoretical basis for the selective breeding of red claw crayfish.

Key words: red claw crayfish, phenotypic characters, sexual dimorphism, correlation coefficient, regression analysis

中图分类号:

S966.12

陈红林, 秦高婵, 楼宝, 钱豪杰, 姚振海. 不同生长时期红螯螯虾表型性状差异分析[J]. 浙江农业学报, 2020, 32(12): 2154-2161.

CHEN Honglin, QIN Gaochan, LOU Bao, QIAN Haojie, YAO Zhenhai. Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2154-2161.

图/表 6

参考文献 25

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性状 Trait	月龄 Month	平均值 Mean	变异系数 CV/%
Y₁/g	3	19.30	28.03
	4.5	40.86	21.39
	6	62.75	24.57
Y₂/g	3	1.32	0.29
	4.5	2.75	0.26
	6	4.30	0.24
X₁/mm	3	94.44	10.11
	4.5	122.65	8.10
	6	138.72	7.76
X₂/mm	3	45.67	9.81
	4.5	59.17	8.01
	6	67.66	8.20
X₃/mm	3	18.93	12.04
	4.5	24.57	8.22
	6	29.10	8.52
X₄/mm	3	32.93	9.75
	4.5	42.57	7.56
	6	48.14	7.19
X₅/mm	3	7.62	10.37
	4.5	9.79	8.07
	6	11.72	14.51
X₆/mm	3	18.13	9.93
	4.5	23.97	9.01
	6	27.99	9.90
X₇/mm	3	28.44	11.60
	4.5	39.32	12.03
	6	50.35	12.95

性状 Trait	月龄 Month	平均值 Mean	变异系数 CV/%
Y₁/g	3	19.30	28.03
	4.5	40.86	21.39
	6	62.75	24.57
Y₂/g	3	1.32	0.29
	4.5	2.75	0.26
	6	4.30	0.24
X₁/mm	3	94.44	10.11
	4.5	122.65	8.10
	6	138.72	7.76
X₂/mm	3	45.67	9.81
	4.5	59.17	8.01
	6	67.66	8.20
X₃/mm	3	18.93	12.04
	4.5	24.57	8.22
	6	29.10	8.52
X₄/mm	3	32.93	9.75
	4.5	42.57	7.56
	6	48.14	7.19
X₅/mm	3	7.62	10.37
	4.5	9.79	8.07
	6	11.72	14.51
X₆/mm	3	18.13	9.93
	4.5	23.97	9.01
	6	27.99	9.90
X₇/mm	3	28.44	11.60
	4.5	39.32	12.03
	6	50.35	12.95

生长时期	X₂/X₁	X₃/X₁	X₄/X₁	X₅/X₁	X₆/X₁	X₇/X₁	Y₂/Y₁
Growth stage
3月龄3 months	48.89±0.97 a	20.31±0.73 b	34.33±0.99 a	7.99±0.50 b	18.84±0.63 b	31.26±2.27 c	7.03±1.05 a
4.5月龄4.5 months	48.28±1.11 a	20.05±0.65 b	34.72±1.08 a	7.99±0.48 b	19.54±0.74 b	32.07±2.76 b	6.79±1.29 a
6月龄 6 months	48.77±1.54 a	21.00±1.20 a	34.74±1.30 a	8.47±1.21 a	20.20±1.55 a	36.21±2.80 a	6.98±1.20 a

生长时期	X₂/X₁	X₃/X₁	X₄/X₁	X₅/X₁	X₆/X₁	X₇/X₁	Y₂/Y₁
Growth stage
3月龄3 months	48.89±0.97 a	20.31±0.73 b	34.33±0.99 a	7.99±0.50 b	18.84±0.63 b	31.26±2.27 c	7.03±1.05 a
4.5月龄4.5 months	48.28±1.11 a	20.05±0.65 b	34.72±1.08 a	7.99±0.48 b	19.54±0.74 b	32.07±2.76 b	6.79±1.29 a
6月龄 6 months	48.77±1.54 a	21.00±1.20 a	34.74±1.30 a	8.47±1.21 a	20.20±1.55 a	36.21±2.80 a	6.98±1.20 a

性状Trait	性别Sex	3月龄3 months	4.5月龄4.5 months	6月龄6 months
Y₁	雌Female	24.44±7.07	41.04±8.34	57.93±17.24^*
	雄Male	24.77±6.70	40.44±9.31	65.45±13.92
Y₂	雌Female	1.72±0.61	2.96±0.72^**	4.13±0.99
	雄Male	1.74±0.50	2.53±0.65	4.4±1.03
X₁	雌Female	103.02±11.47	123.99±9.44	137.94±14.09
	雄Male	103.05±10.63	121.34±10.41	139.42±8.41
X₂	雌Female	50.24±5.82	59.48±4.66	66.81±6.30
	雄Male	50.75±5.78	58.79±4.90	68.42±4.62
X₃	雌Female	20.87±2.62	24.92±1.94	29.25±3.21
	雄Male	21.17±2.67	24.19±2.10	29.04±2.03
X₄	雌Female	35.44±3.53	42.94±3.04	49.05±4.43
	雄Male	35.03±3.33	42.06±3.39	47.66±2.74
X₅	雌Female	8.21±1.00	9.81±0.81	12.66±2.37^**
	雄Male	8.24±0.88	9.73±0.79	11.19±0.86
X₆	雌Female	19.49±2.02	24.65±2.20^**	29.96±3.40^**
	雄Male	19.07±1.74	23.23±1.93	26.92±1.57
X₇	雌Female	31.92±4.60	38.58±4.74	48.16±6.92^*
	雄Male	32.95±4.94	40.07±4.78	51.60±6.08

不同生长时期红螯螯虾表型性状差异分析

Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages

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性状Trait	Y₁	Y₂	X₁	X₂	X₃	X₄	X₅	X₆	X₇
Y₁	1.00	0.81	0.98^**	0.98^**	0.98^**	0.97^**	0.84	0.97^**	0.54
Y₂	0.79	1.00	0.79	0.79	0.82	0.72	0.63	0.77	0.47
X₁	0.96^**	0.70	1.00	0.98^**	0.95^**	0.97^**	0.84	0.97^**	0.46
X₂	0.95^**	0.68	0.97^**	1.00	0.96^**	0.97^**	0.84	0.98^**	0.49
X₃	0.94^**	0.70	0.94^**	0.94^**	1.00	0.94^**	0.81	0.95^**	0.52
X₄	0.96^**	0.79	0.94^**	0.92^**	0.91^**	1.00	0.87^**	0.98^**	0.44
X₅	0.82	0.71	0.81	0.82	0.78	0.86^**	1.00	0.87^**	0.39
X₆	0.94^**	0.80	0.94^**	0.91^**	0.90^**	0.95^**	0.84	1.00	0.44
X₇	0.77	0.79	0.81	0.76	0.70	0.68	0.70	0.52	1.00

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