浙江农业学报 ›› 2023, Vol. 35 ›› Issue (5): 1001-1015.DOI: 10.3969/j.issn.1004-1524.2023.05.04

• 作物科学 • 上一篇    下一篇

油橄榄种质资源表型性状多样性分析与评价

戚建莉1,2(), 张荣1,2, 吴文俊1,2, 姜成英1,2,*(), 赵梦炯1,2, 金高明1,2, 陈炜青1,2   

  1. 1.甘肃省林业科学研究院 油橄榄工程技术研究中心,甘肃 兰州730020
    2.国家林业草原油橄榄工程技术研究中心,甘肃 兰州730020
  • 收稿日期:2022-08-31 出版日期:2023-05-25 发布日期:2023-06-01
  • 作者简介:戚建莉(1986—),女,甘肃景泰人,学士,高级工程师,研究方向为经济林研究。E-mail: 316760156@qq.com
  • 通讯作者: *姜成英,E-mail: jcytxb@126.com
  • 基金资助:
    甘肃省林草局自列项目(2021kj044);甘肃省林草局创新项目(kjcx202005);甘肃省科技计划(21CX6NA041)

Analysis and evaluation of phenotypic character diversity of olive germplasm resources

QI Jianli1,2(), ZHANG Rong1,2, WU Wenjun1,2, JIANG Chengying1,2,*(), ZHAO Mengjiong1,2, JIN Gaoming1,2, CHEN Weiqing1,2   

  1. 1. Center for Olive Research and Technology, Gansu Academy of Forestry Sciences, Lanzhou 730020, China
    2. National Forestry Grassland Olive Engineering Technology Research Center, Lanzhou 730020, China
  • Received:2022-08-31 Online:2023-05-25 Published:2023-06-01

摘要:

探明甘肃地区油橄榄种质资源表型性状的遗传多样性和变异特点,旨在为当地油橄榄种质资源选育、评价和开发利用提供理论参考。对35份油橄榄种质资源的11个特征进行描述,对16个表型性状进行多样性、相关性、主成分、聚类等分析。结果表明:不同油橄榄种质表型性状之间存在显著差异,其变异系数变幅较大,介于8.64%~55.87%,单果重变异系数最大,果肉率变异系数最小。相关性分析发现,果实纵径、果实横径、单果重、果肉厚、果核纵径、果核横径、果核重和果肉率相互间均呈极显著正相关(P<0.01)。主成分分析发现,16个表型性状可被提取为5个主成分,累积贡献率达到91.633%。聚类分析表明,在欧氏距离10处可将35份油橄榄种质资源分成5类,可作为5个不同育种方向,其中切姆拉里因其干鲜果含油率最高单独聚为一类,可作为特异种质进行挖掘利用;M1果实水分最少,干鲜果含油率仅次于切姆拉里,划分为油用类;贝拉和绿源小苹果果实水分最多,鲜果含油率低,划分为果用类;其余2类种质可结合其他生理指标进行挖掘利用。综上所述,不同油橄榄种质间表型性状差异较大,生物多样性较为丰富,小尼、绿源小苹果、M1、切姆拉里等种质表型性状突出,可作为亲本选配;单果重、果形指数、果核指数、鲜果含油率、干果含油率等7个指标可以作为油橄榄种质资源评选和选育鉴定工作的重要指标。

关键词: 油橄榄, 种质资源, 表型性状, 多样性

Abstract:

In order to explore the genetic diversity and variation characteristics of phenotypic traits of olive germplasm resources in Gansu Province, and to provide a theoretical reference for the selection, evaluation, development and utilization of local olive germplasm resources, 11 characters of 35 olive germplasm resources were described, and 16 phenotypic characters were analyzed by diversity, correlation, principal component analysis and cluster analysis. The results showed that there were significant differences among the phenotypic characters of different olive germplasm resources, and the coefficient of variation ranged from 8.64% to 55.87%. The variation coefficient of single fruit weight was the largest, and the variation coefficient of flesh rate was the smallest. Correlation analysis showed that fruit longitudinal diameter, fruit transverse diameter, single fruit weight, pulp thickness, fruit core longitudinal diameter, fruit core transverse diameter, fruit core weight and pulp rate were significantly (P <0.01) positively correlated with each other. Principal component analysis showed that 16 phenotypic traits could be extracted into 5 principal components, with a cumulative contribution rate of 91.633%. Cluster analysis showed that 35 olive germplasm resources could be divided into 5 categories at Euclidean distances of 10, which could be used as 5 different breeding directions. Among them, the highest oil content of dried and fresh fruits of Chemlal de kabylie could be grouped into one category, which could be used as special germplasm for mining; M1 had the least water content, and the oil content of fresh and dry fruits was only lower than that of Chemlal de kabylie, which was divided into oil products; Berat and LYuan Manzanilla had the highest water content and low oil content in fresh fruit, which could be divided into fruit products; The other two types of germplasm could be excavated and utilized in combination with other physiological indicators. This study showed that the phenotypic traits of different olive germplasm resources were significantly different, and the biodiversity was rich. The phenotypic traits of Nikiskii, LYuan Manzanilla, M1, Chemlal de kabylie and other germplasm were prominent, which could be used as parents for selection. Through correlation analysis, 7 indexes, such as single fruit weight, fruit shape index, stone index, fresh fruit oil rate and dry fruit oil rate, were selected as important indexes for selection, breeding and identification of olive germplasm resources.

Key words: olive, germplasm resource, phenotypic traits, diversity

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