兔流行性腹胀病病原分离鉴定与防治

doi:10.3969/j.issn.1004-1524.2021.11.05

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (11): 2034-2040.DOI: 10.3969/j.issn.1004-1524.2021.11.05

兔流行性腹胀病病原分离鉴定与防治

季权安(), 刘燕, 肖琛闻, 黄叶娥, 李科, 韦强, 鲍国连^*()

浙江省农业科学院畜牧兽医研究所,浙江杭州 310021

收稿日期:2021-03-15 出版日期:2021-11-25 发布日期:2021-11-26
通讯作者: 鲍国连
作者简介:*鲍国连,E-mail: Baogl@zaas.org
季权安(1967—),男,浙江庆元人,学士,高级实验师,主要从事畜禽传染病诊断与防治研究。E-mail: jiquanan@hotmail.com
基金资助:
国家现代农业产业技术体系(CARS-43-C-2);浙江省重点研发计划(2016C02054-10);浙江省重点研发计划(2019C02052)

Isolation, identification and control of rabbit epidemic abdominal distension

JI Quan’an(), LIU Yan, XIAO Chenwen, HUANG Ye’e, LI Ke, WEI Qiang, BAO Guolian^*()

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-03-15 Online:2021-11-25 Published:2021-11-26
Contact: BAO Guolian

摘要/Abstract

摘要：

为了有效控制家兔流行性腹胀病的蔓延,结合流行病学调查和实验室鉴别诊断技术,对112例40~90日龄患病兔病样开展病原检测,其中,分离鉴定出大肠埃希菌(Escherichia coli)的有78例,分离鉴定出魏氏梭菌(Clostridium welchii)的有69例。致病性试验表明,这两大分离菌为兔流行性腹胀病的主要致病菌。在3个常发病兔场开展溶菌酶临床防治试验。结果显示,无论是预防试验中的发病率,还是治疗试验中的病死率,溶菌酶处理组均比对照组低50多个百分点,表明溶菌酶对该病的防治效果明显,能有效遏制病情蔓延,可作为当前替代抗生素的首选药物。研究结果为兔流行性腹胀病的防治提供了科学依据。

关键词: 兔, 流行性腹胀病, 细菌分离, 溶菌酶

Abstract:

In order to effectively control the spread of epidemic abdominal distension in rabbits, epidemiological investigation and laboratory differential diagnosis technology were applied in the present study. Pathogen detection was carried out on 112 cases of 40-90-d-old diseased rabbits. Among them, Escherichia coli and Clostridium welchii were isolated and identified from 78 and 69 diseased rabbits, respectively. The pathogenic characteristics test showed that these two species were the main pathogens of epidemic abdominal distension in rabbits. The clinical control trials of lysozyme were carried out in 3 rabbit farms suffering from epidemic abdominal distension. It was shown that either the incidence rate in the prevention trial or the mortality rate in the control trial was lowered by 50 percentage points in the treatment group than that in the control group. The results indicated that lysozyme could prevent and treat the disease, hence effectively curb the spread of the disease. Therefore, it could be used as the substitution of antibiotics. These findings provided scientific basis for the prevention and treatment of rabbit epidemic abdominal distension.

Key words: rabbit, epidemic abdominal distension, bacterial isolation, lysozyme

中图分类号:

S858.291

季权安, 刘燕, 肖琛闻, 黄叶娥, 李科, 韦强, 鲍国连. 兔流行性腹胀病病原分离鉴定与防治[J]. 浙江农业学报, 2021, 33(11): 2034-2040.

JI Quan’an, LIU Yan, XIAO Chenwen, HUANG Ye’e, LI Ke, WEI Qiang, BAO Guolian. Isolation, identification and control of rabbit epidemic abdominal distension[J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2034-2040.

图/表 6

参考文献 21

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指标 Index	LH	JS	FY	合计 Total
患病兔数Number of sick rabbits	41	38	33	112
大肠埃希菌分离数	29	26	23	78
Number of Escherichia coli infection
魏氏梭菌分离数	25	23	21	69
Number of Clostridium welchii infection
混合感染分离数	24	21	17	62
Number of mixed infection
球虫感染分离数	3	2	1	6
Number of coccidiosis infection
未定型杂菌分离数	2	1	1	4
Number of unformed bacteria infection

指标 Index	LH	JS	FY	合计 Total
患病兔数Number of sick rabbits	41	38	33	112
大肠埃希菌分离数	29	26	23	78
Number of Escherichia coli infection
魏氏梭菌分离数	25	23	21	69
Number of Clostridium welchii infection
混合感染分离数	24	21	17	62
Number of mixed infection
球虫感染分离数	3	2	1	6
Number of coccidiosis infection
未定型杂菌分离数	2	1	1	4
Number of unformed bacteria infection

试验地 Test site	组别 Group	预防数 Prevention number	发病数 Number of case	发病率 Incidence rate/%
LH	预防组Prevention group	45	6	13.3
	对照组Control group	45	29	64.4
JS	预防组Prevention group	45	4	8.9
	对照组Control group	45	27	60.0
FY	预防组Prevention group	45	2	4.4
	对照组Control group	45	25	55.6

试验地 Test site	组别 Group	预防数 Prevention number	发病数 Number of case	发病率 Incidence rate/%
LH	预防组Prevention group	45	6	13.3
	对照组Control group	45	29	64.4
JS	预防组Prevention group	45	4	8.9
	对照组Control group	45	27	60.0
FY	预防组Prevention group	45	2	4.4
	对照组Control group	45	25	55.6

试验地 Test site	组别 Group	患病数 Number of cases	病死数 Number of deaths	病死率 Mortality rate/%
LH	治疗组Treatment group	45	7	15.6
	对照组Control group	45	42	93.3
JS	预防组Prevention group	45	5	11.1
	对照组Control group	45	40	88.9
FY	预防组Prevention group	45	3	6.7
	对照组Control group	45	38	84.4

兔流行性腹胀病病原分离鉴定与防治

Isolation, identification and control of rabbit epidemic abdominal distension

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