›› 2010, Vol. 22 ›› Issue (1): 0-90.

• 论文 •    

不同猪瘟疫苗类型及剂量的抗体反应特性研究

毛文杰1,2,陈宁1,曲健3,唐宇龙1,方维焕1,*   

  1. 1浙江大学 浙江省动物预防医学重点实验室,浙江 杭州 310029;2浙江省舟山市普陀区畜牧兽医局, 浙江 舟山 316100;3浙江省兽药饲料监测所,浙江 杭州 310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-25 发布日期:2010-01-25

Antibody responses of pigs to different types and doses of Hog cholera vaccines

MAO Wen-jie;CHEN Ning;QU Jian;TANG Yu-long;FANG Wei-huan;*   

  1. 1 Zhejiang University, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 310029, China; 2 Putuo Animal Husbandry and Veterinary Administration, Zhoushan 316100, China;3 Zhejiang Institute of Quality Control of Veterinary Pharmaceuticals and Animal Feeds, Hangzhou 310029, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-25 Published:2010-01-25

摘要: 随机选择3个猪场, 从9头母猪生下的仔猪中挑选81头仔猪。随机分成9组,每组又分为3个小组,每小组3头,分别免疫猪瘟组织苗(A)、猪瘟细胞苗(B)和猪瘟脾淋苗(C),各疫苗分别采用1头份、2头份和3头份3个剂量。于25日龄首免疫前后不同时间点采血,分离血清。应用ELISA和IHA两种方法分别对采集的540份血清检测猪瘟抗体。不同类型的猪瘟疫苗免疫试验结果表明:猪瘟脾淋苗免疫效果较好,但不同疫苗之间的抗体水平差异不显著。不同免疫剂量的试验结果显示:3种疫苗以3头份的剂量免疫后抗体反应略高于1头份和2头份,二免后都能获得较高的抗体水平。

关键词: 猪瘟疫苗, 抗体水平, 酶联免疫吸附试验, 正向间接血凝试验

Abstract: Eighty-one piglets, which derived from 9 sows of 3 different farms were divided into 9 groups, 27 subgroups, with 3 piglets for each subgroup. Each group was vaccinated with Hog cholera tissue vaccine (A), cell vaccine (B) or spleen vaccine (C) with three dose levels (1×, 2×or 3×recommended doses). Blood samples were collected from the 25th day after vaccination for serum. Antibodies were tested by E2 protein based on ELISA and IHA. The results showed that spleen vaccine seemed to be better than the other two though there was no significant difference among them. Higher dose (3×recommended dose) generally induced higher antibody response than lower doses. Higher antibody level was observed after the boosting vaccination.

Key words: Hog cholera vaccine, antibody response, ELISA, indirect hemagglutination test