›› 2010, Vol. 22 ›› Issue (1): 0-5.

• 论文 •    

一个矮秆多分蘖突变体的遗传分析和定位

李克磊1,2,汪得凯2,*,陶跃之2   

  1. 1杭州师范大学 生命与环境科学学院,浙江 杭州,310036;2浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-25 发布日期:2010-01-25

Genetic analysis and mapping of a mutant gene for high tillering and dwarf in rice (Oryza sativa L.)

LI Ke-lei;WANG De-kai;*;TAO Yue-zhi   

  1. 1 College of Life and Environmental Sciences, Hangzhou Normal University,Hangzhou 310036, China;2 Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-25 Published:2010-01-25

摘要: 从粳稻品种‘中花11’转基因后代中发现了一个矮秆多分蘖突变体,突变体表现出植株矮化、分蘖力强 、穗长变短、叶片变窄及结实率降低等一系列突变表型。遗传分析表明该矮秆多分蘖突变体表型受一对隐性核基因控制,因其与突变体htd1具有相类似的表型,故将该基因命名为htd2。利用籼粳杂交F2群体将突变基因定位在第3染色体短臂上SSR标记 RM6038和RM5444之间,与两个标记的遗传距离分别为1.4和2.1 cM,两个标记之间的物理距离大约为400 kb。

关键词: 水稻, 矮化多分蘖突变基因, 遗传分析, 图位克隆

Abstract: A rice mutant, showing the phenotype of high-tillering and dwarfing, was discovered from a population derived from a transgenic japonica cultivar ‘Zhonghua11’. The genetic analysis indicated that the phenotype of the mutant was controlled by a single recessive gene, which was designated tentatively as htd2. The F2 population was developed from a cross between the mutant and an indica cultivar ‘Longtefu B’ and used to investigate the genetic model and map the gene. The htd2 gene was firstly mapped on the short arm of chromosome3 and further mapped on the genetic region between SSR marker RM6038 and RM5444, which was 400 kb in length.

Key words: rice (Oryza sativa L.), high-tillering and dwarf mutant, genetic analysis, map-based cloning