铜-菲复合污染对分蘖期水稻根系生理特性和污染物积累的影响

doi:10.3969/j.issn.1004-1524.20240168

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (3): 521-529.DOI: 10.3969/j.issn.1004-1524.20240168

铜-菲复合污染对分蘖期水稻根系生理特性和污染物积累的影响

宋欣录¹(), 范书红², 武桄旗¹, 展梦琪¹, 侯倩¹, 李明月¹, 徐艳¹^,^*()

1.青岛大学环境科学与工程学院,山东青岛 266071
2.枣庄市农业农村事业发展中心,山东枣庄 277099

收稿日期:2024-02-29 出版日期:2025-03-25 发布日期:2025-04-02
作者简介:宋欣录(1999—),男,山东泰安人,硕士研究生,研究方向为土壤微生物学。E-mail:song1xinlu@163.com
通讯作者: * 徐艳,E-mail:yanxu@qdu.edu.cn
基金资助:
国家自然科学基金(42177028);山东省高等学校青创人才引育计划(DC2000000961)

Effects of combined copper-phenanthrene pollution on physiological characteristics and pollutant accumulation of rice roots at tillering stage

SONG Xinlu¹(), FAN Shuhong², WU Guangqi¹, ZHAN Mengqi¹, HOU Qian¹, LI Mingyue¹, XU Yan¹^,^*()

1. School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, Shandong, China
2. Zaozhuang Agricultural and Rural Business Development Center, Zaozhuang 277099, Shandong, China

Received:2024-02-29 Online:2025-03-25 Published:2025-04-02

摘要/Abstract

摘要：

土壤污染中经常存在重金属与多环芳烃复合污染的情况,污染物通过根系进入植物体内,严重危害作物生产安全。为探究复合污染对作物的影响,采用盆栽试验,研究菲、铜和铜-菲复合污染下分蘖期水稻生理生化特性和根系中铜与菲的积累特征。结果表明,复合污染下水稻的电解质渗透率较菲污染处理高出16.08百分点;污染处理的水稻根系过氧化物酶和过氧化氢酶活性均显著(P<0.05)降低,但复合污染与单一污染处理之间无显著差异。复合污染下水稻根内与根际的菲含量分别为0.59 mg·kg^-1和6.51 mg·kg^-1,显著(P<0.05)高于菲污染处理(0.01 mg·kg^-1和3.63 mg·kg^-1);水稻根系的铜含量为48.28~74.18 mg·kg^-1,复合污染与单一污染处理的铜含量无显著差异。综上,铜-菲复合污染并未加强对水稻抗氧化酶活性的抑制,但显著增加了水稻根系与根际土壤中的菲含量,加重菲污染。

关键词: 水稻, 迁移转化, 铜, 菲, 复合污染

Abstract:

Soil pollution often involves a combination of heavy metals and polycyclic aromatic hydrocarbon, pollutants enter the plant through the root system, seriously endangering the safety of crop production. In order to study the effect of compound pollution on crops, pot experiment was carried out to analyze the effects of single additions of phenanthrene and copper, as well as their combined addition, on rice physiological and biochemical properties and copper and phenanthrene accumulation in rice roots at tillering stage. The results showed that the electrolyte permeability of rice under combined pollution of phenanthrene and copper was 16.08 percentage points higher than phenanthrene treatment; the activities of peroxidase and catalase in rice root were significantly (P<0.05) decreased under the pollution treatment, but there was no significant difference between combined pollution treatment and single pollution treatment. The content of phenanthrene in rice roots and rhizosphere under combined pollution of copper and phenanthrene were 0.59 mg·kg^-1 and 6.51 mg·kg^-1, respectively, which were significantly (P<0.05) higher than those in phenanthrene treatment (0.01 mg·kg^-1 and 3.63 mg·kg^-1). The content of copper in the rice roots was ranged from 48.28 to 74.18 mg·kg^-1, there was no significant difference in copper content between the combined pollution treatment and the single pollution treatment. In conclusion, copper-phenanthrene combined pollution did not enhance the inhibition of antioxidant enzyme activities in rice, significantly increased the content of phenanthrene in rice root and rhizosphere soil, and aggravated phenanthrene pollution.

Key words: rice, transport and transfer, copper, phenanthrene, combined pollution

中图分类号:

S511
X173

宋欣录, 范书红, 武桄旗, 展梦琪, 侯倩, 李明月, 徐艳. 铜-菲复合污染对分蘖期水稻根系生理特性和污染物积累的影响[J]. 浙江农业学报, 2025, 37(3): 521-529.

SONG Xinlu, FAN Shuhong, WU Guangqi, ZHAN Mengqi, HOU Qian, LI Mingyue, XU Yan. Effects of combined copper-phenanthrene pollution on physiological characteristics and pollutant accumulation of rice roots at tillering stage[J]. Acta Agriculturae Zhejiangensis, 2025, 37(3): 521-529.

图/表 5

图1 水稻根系的电解质渗透率

Fig.1 Electrolyte leakage of rice roots

图2 水稻根系的抗氧化酶活性数据以鲜重计。

Fig.2 Activities of antioxidant enzymes in rice roots Data was detected based on fresh weight.

图3 水稻根靶区域显微镜扫描图和LA-ICP-MS铜元素分布扫描图 A,水稻根的纵切面靶区域显微镜扫描图;B,水稻根的横切面靶区域显微镜扫描图;C,水稻根的纵切面LA-ICP-MS铜元素分布色块;D,水稻根的横切面LA-ICP-MS铜元素分布色块。

Fig.3 Microscope scanning of target area and copper distribution scanning by LA-ICP-MS for rice roots A, Microscope scanning of target area in longitudinal section of rice root; B, Microscope scanning of target area in transverse section of rice root; C, LA-ICP-MS copper distribution colormapof longitudinal section of rice root; D, LA-ICP-MS copper distribution colormap of transverse section of rice root.

图4 光学照相机下水稻根尖样品形貌和根中元素含量分布色块图分辨率:2 568 pixel×1 230 pixel;采样步长:18 μm;单点驻留时间:10 ms。

Fig.4 The image of rice root tips under optical camera and the content distribution colormap of elements in rice roots Image resolution: 2 568 pixel×1 230 pixel; Size: 18 μm; Time:10 ms.

图5 不同根区内菲、全铜和有效铜的含量

Fig.5 The contents of phenanthrene, total copper and available copper in different rhizocompartments

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铜-菲复合污染对分蘖期水稻根系生理特性和污染物积累的影响

Effects of combined copper-phenanthrene pollution on physiological characteristics and pollutant accumulation of rice roots at tillering stage

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