利用染色体片段替换系鉴定水稻二化螟抗性QTL

doi:10.3969/j.issn.1004-1524.20240217

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (3): 530-537.DOI: 10.3969/j.issn.1004-1524.20240217

利用染色体片段替换系鉴定水稻二化螟抗性QTL

雷志伟¹^,²(), 李新欣²^,³, 徐恒², 张恒², 朱英², 张华²^,^*()

1.浙江农林大学现代农学院,浙江杭州 311300
2.浙江省农业科学院病毒学与生物技术研究所,农产品质量安全全国重点实验室,浙江省作物分子育种工程技术研究中心,浙江杭州 310021
3.浙江师范大学化学与生命科学学院,浙江金华 321004

收稿日期:2024-03-08 出版日期:2025-03-25 发布日期:2025-04-02
作者简介:雷志伟(1995—),男,山西朔州人,硕士研究生,主要从事水稻抗虫基因功能研究。E-mail:1364641389@qq.com
通讯作者: * 张华,E-mail:zhanghua2011@whu.edu.cn
基金资助:
国家重点研发计划(2023YFD1200901)

Identification of QTLs for stem borer resistance using chromosome segment substitution lines in rice

LEI Zhiwei¹^,²(), LI Xinxin²^,³, XU Heng², ZHANG Heng², ZHU Ying², ZHANG Hua²^,^*()

1. College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China
2. State Key Laboratory for Quality and Safety of Agro-Products, Research Center for Crop Molecular Breeding at Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, Zhejiang, China

Received:2024-03-08 Online:2025-03-25 Published:2025-04-02

摘要/Abstract

摘要：

螟虫灾害对我国水稻生产危害较重,而水稻二化螟抗性的遗传研究却较为匮乏,不利于抗螟虫水稻品种的培育。本研究利用一套以粳稻日本晴为供体、籼稻9311为受体的染色体片段替换系为材料,对水稻二化螟抗性性状进行遗传分析,在水稻基因组鉴定出5个与二化螟抗性相关的数量性状基因座(quantitative trait loci, QTL)。通过对QTL相关替换系的表型分析,发现4个来源于粳稻日本晴的QTL(qRSB1、qRSB3、qRSB4和qRSB6)可以显著提高籼稻9311对二化螟的抗性。同时,根据相关替换系的基因型鉴定结果,通过物理定位初步明确了各个QTL在水稻基因组中的候选区间。以上研究结果可以为这些QTL的下一步精细定位和候选基因的分离打下研究基础,也可以为二化螟抗性水稻品种的培育提供必要的鉴定方法和理论指导。

关键词: 水稻二化螟, 染色体片段替换系, QTL定位, 分子标记

Abstract:

The rice stem borer disaster causes serious damage to rice production in China. However, the genetic mechanisms of stem borer resistance in rice is still unknown, and the breeding of stem borer resistant rice variety faces big challenges. In this study, a set of chromosome segment substitution lines with japonica rice ‘Nipponbare’ as the donor and indica rice ‘9311’ as the recipient were used as materials to conduct a genetic analysis of the traits related to resistance to the rice stem borer. Five quantitative trait loci (QTL) related to resistance to the rice stem borer were identified in the rice genome. Through phenotypic analysis of the relevant substitution lines, it was found that four QTLs derived from japonica rice ‘Nipponbare’ (qRSB1, qRSB3, qRSB4, and qRSB6) could significantly enhance the resistance of indica rice ‘9311’ to the rice stem borer. Meanwhile, based on the genotypic identification results of the relevant substitution lines, the candidate intervals of each QTL in the rice genome were initially determined through physical mapping. The above research results provide a basis for the further fine mapping and candidate gene isolation of these stem borer resistance QTLs and would be beneficial to the breeding of stem borer resistant rice varieties in future.

Key words: rice stem borer, chromosome segment substitution line, QTL mapping, molecular marker

中图分类号:

S511

雷志伟, 李新欣, 徐恒, 张恒, 朱英, 张华. 利用染色体片段替换系鉴定水稻二化螟抗性QTL[J]. 浙江农业学报, 2025, 37(3): 530-537.

LEI Zhiwei, LI Xinxin, XU Heng, ZHANG Heng, ZHU Ying, ZHANG Hua. Identification of QTLs for stem borer resistance using chromosome segment substitution lines in rice[J]. Acta Agriculturae Zhejiangensis, 2025, 37(3): 530-537.

图/表 6

图1 粳稻日本晴(NIP)和籼稻9311在田间和网室的二化螟抗性鉴定结果 A、B,日本晴和9311在田间的二化螟抗性鉴定结果,水稻灌浆后期植株死亡率越高表示对二化螟越敏感;C,日本晴和9311在网室的二化螟抗性鉴定结果,人工接虫后,水稻植株枯心指数越高表示对二化螟越敏感。**表示在P<0.01水平差异显著。下同。

Fig.1 Rice stem borer resistance results of japonica cultivar Nipponbare (NIP) and indica variety 9311 in field and netting house A and B, Rice stem borer resistance results of Nipponbare and 9311 in field condition, the higher the mortality rate of rice plants during the late filling stage indicates greater sensitivity to rice stem borer; C, Rice stem borer resistance results of Nipponbare and 9311 in netting house, after manual infestation, the higher the dead heart index of rice plants indicates greater sensitivity to rice stem borer. ** means significant differences at the levels of P<0.01. The same as below

图2 水稻染色体片段替换系的基因型检测结果空白处表示9311背景,蓝色区表示日本晴替换片段,红色表示杂合区。

Fig.2 Genotype of chromosome segment substitution lines of rice The blank represents background of 9311, the blue region represents substitution chromosome segment from Nipponbare, and the red region represents heterozygous genotype.

图3 水稻染色体片段替换系的田间抗螟虫性状统计

Fig.3 Statistical results of rice chromosome segment substitution lines resistant to stem borer

图4 水稻二化螟抗性QTL分析结果

Fig.4 Analysis results of QTLs for rice stem borer resistance

表1 水稻二化螟抗性QTL的LOD值和连锁分子标记

Table 1 LOD score and linked molecular marker of QTL for rice stem borer resistance

QTL	LOD值 LOD score	连锁分子标记 Linked molecular marker
qRSB1	5.343	CS0138
qRSB3	4.608	CS0333
qRSB4	5.142	R4M43
qRSB6	5.067	CS0610
qRSB10	4.483	CS1002

图5 水稻二化螟抗性QTL(qRSB1、qRSB3、qRSB4、qRSB6和qRSB10)的物理定位结果空白处表示9311背景,黑色区表示日本晴替换片段。R和S分别表示二化螟抗性较强(L3)和较弱(L1)。

Fig.5 Physical mapping results of QTLs (qRSB1, qRSB3, qRSB4, qRSB6 and qRSB10) for rice stem borer resistance The blank represents background of 9311, the black region represents substitution chromosome segment from Nipponbare. R and S represent resistant(L3)/susceptible(L1) to rice stem borer, respectively.

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利用染色体片段替换系鉴定水稻二化螟抗性QTL

Identification of QTLs for stem borer resistance using chromosome segment substitution lines in rice

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