利用染色体片段替换系鉴定水稻二化螟抗性QTL

doi:10.3969/j.issn.1004-1524.20240217

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (3): 530-537.DOI: 10.3969/j.issn.1004-1524.20240217

利用染色体片段替换系鉴定水稻二化螟抗性QTL

雷志伟¹^,²(), 李新欣²^,³, 徐恒², 张恒², 朱英², 张华²^,^*()

1.浙江农林大学现代农学院,浙江杭州 311300
2.浙江省农业科学院病毒学与生物技术研究所,农产品质量安全全国重点实验室,浙江省作物分子育种工程技术研究中心,浙江杭州 310021
3.浙江师范大学化学与生命科学学院,浙江金华 321004

收稿日期:2024-03-08 出版日期:2025-03-25 发布日期:2025-04-02
作者简介:雷志伟(1995—),男,山西朔州人,硕士研究生,主要从事水稻抗虫基因功能研究。E-mail:1364641389@qq.com
通讯作者: * 张华,E-mail:zhanghua2011@whu.edu.cn
基金资助:
国家重点研发计划(2023YFD1200901)

Identification of QTLs for stem borer resistance using chromosome segment substitution lines in rice

LEI Zhiwei¹^,²(), LI Xinxin²^,³, XU Heng², ZHANG Heng², ZHU Ying², ZHANG Hua²^,^*()

1. College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China
2. State Key Laboratory for Quality and Safety of Agro-Products, Research Center for Crop Molecular Breeding at Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, Zhejiang, China

Received:2024-03-08 Online:2025-03-25 Published:2025-04-02

摘要/Abstract

摘要：

螟虫灾害对我国水稻生产危害较重,而水稻二化螟抗性的遗传研究却较为匮乏,不利于抗螟虫水稻品种的培育。本研究利用一套以粳稻日本晴为供体、籼稻9311为受体的染色体片段替换系为材料,对水稻二化螟抗性性状进行遗传分析,在水稻基因组鉴定出5个与二化螟抗性相关的数量性状基因座(quantitative trait loci, QTL)。通过对QTL相关替换系的表型分析,发现4个来源于粳稻日本晴的QTL(qRSB1、qRSB3、qRSB4和qRSB6)可以显著提高籼稻9311对二化螟的抗性。同时,根据相关替换系的基因型鉴定结果,通过物理定位初步明确了各个QTL在水稻基因组中的候选区间。以上研究结果可以为这些QTL的下一步精细定位和候选基因的分离打下研究基础,也可以为二化螟抗性水稻品种的培育提供必要的鉴定方法和理论指导。

关键词: 水稻二化螟, 染色体片段替换系, QTL定位, 分子标记

Abstract:

The rice stem borer disaster causes serious damage to rice production in China. However, the genetic mechanisms of stem borer resistance in rice is still unknown, and the breeding of stem borer resistant rice variety faces big challenges. In this study, a set of chromosome segment substitution lines with japonica rice ‘Nipponbare’ as the donor and indica rice ‘9311’ as the recipient were used as materials to conduct a genetic analysis of the traits related to resistance to the rice stem borer. Five quantitative trait loci (QTL) related to resistance to the rice stem borer were identified in the rice genome. Through phenotypic analysis of the relevant substitution lines, it was found that four QTLs derived from japonica rice ‘Nipponbare’ (qRSB1, qRSB3, qRSB4, and qRSB6) could significantly enhance the resistance of indica rice ‘9311’ to the rice stem borer. Meanwhile, based on the genotypic identification results of the relevant substitution lines, the candidate intervals of each QTL in the rice genome were initially determined through physical mapping. The above research results provide a basis for the further fine mapping and candidate gene isolation of these stem borer resistance QTLs and would be beneficial to the breeding of stem borer resistant rice varieties in future.

Key words: rice stem borer, chromosome segment substitution line, QTL mapping, molecular marker

中图分类号:

S511

雷志伟, 李新欣, 徐恒, 张恒, 朱英, 张华. 利用染色体片段替换系鉴定水稻二化螟抗性QTL[J]. 浙江农业学报, 2025, 37(3): 530-537.

LEI Zhiwei, LI Xinxin, XU Heng, ZHANG Heng, ZHU Ying, ZHANG Hua. Identification of QTLs for stem borer resistance using chromosome segment substitution lines in rice[J]. Acta Agriculturae Zhejiangensis, 2025, 37(3): 530-537.

图/表 6

图1 粳稻日本晴(NIP)和籼稻9311在田间和网室的二化螟抗性鉴定结果 A、B,日本晴和9311在田间的二化螟抗性鉴定结果,水稻灌浆后期植株死亡率越高表示对二化螟越敏感;C,日本晴和9311在网室的二化螟抗性鉴定结果,人工接虫后,水稻植株枯心指数越高表示对二化螟越敏感。**表示在P<0.01水平差异显著。下同。

Fig.1 Rice stem borer resistance results of japonica cultivar Nipponbare (NIP) and indica variety 9311 in field and netting house A and B, Rice stem borer resistance results of Nipponbare and 9311 in field condition, the higher the mortality rate of rice plants during the late filling stage indicates greater sensitivity to rice stem borer; C, Rice stem borer resistance results of Nipponbare and 9311 in netting house, after manual infestation, the higher the dead heart index of rice plants indicates greater sensitivity to rice stem borer. ** means significant differences at the levels of P<0.01. The same as below

图2 水稻染色体片段替换系的基因型检测结果空白处表示9311背景,蓝色区表示日本晴替换片段,红色表示杂合区。

Fig.2 Genotype of chromosome segment substitution lines of rice The blank represents background of 9311, the blue region represents substitution chromosome segment from Nipponbare, and the red region represents heterozygous genotype.

图3 水稻染色体片段替换系的田间抗螟虫性状统计

Fig.3 Statistical results of rice chromosome segment substitution lines resistant to stem borer

图4 水稻二化螟抗性QTL分析结果

Fig.4 Analysis results of QTLs for rice stem borer resistance

表1 水稻二化螟抗性QTL的LOD值和连锁分子标记

Table 1 LOD score and linked molecular marker of QTL for rice stem borer resistance

QTL	LOD值 LOD score	连锁分子标记 Linked molecular marker
qRSB1	5.343	CS0138
qRSB3	4.608	CS0333
qRSB4	5.142	R4M43
qRSB6	5.067	CS0610
qRSB10	4.483	CS1002

图5 水稻二化螟抗性QTL(qRSB1、qRSB3、qRSB4、qRSB6和qRSB10)的物理定位结果空白处表示9311背景,黑色区表示日本晴替换片段。R和S分别表示二化螟抗性较强(L3)和较弱(L1)。

Fig.5 Physical mapping results of QTLs (qRSB1, qRSB3, qRSB4, qRSB6 and qRSB10) for rice stem borer resistance The blank represents background of 9311, the black region represents substitution chromosome segment from Nipponbare. R and S represent resistant(L3)/susceptible(L1) to rice stem borer, respectively.

参考文献 23

[1]	SAVARY S, WILLOCQUET L, PETHYBRIDGE S J, et al. The global burden of pathogens and pests on major food crops[J]. Nature Ecology & Evolution, 2019, 3(3):430-439.
[2]	CHEN M, SHELTON A, YE G Y. Insect-resistant genetically modified rice in China: from research to commercialization[J]. Annual Review of Entomology, 2011, 56: 81-101.
[3]	刘万才, 刘振东, 黄冲, 等. 近10年农作物主要病虫害发生危害情况的统计和分析[J]. 植物保护, 2016, 42(5): 1-9.
	LIU W C, LIU Z D, HUANG C, et al. Statistics and analysis of crop yield losses caused by main diseases and insect pests in recent 10 years[J]. Plant Protection, 2016, 42(5): 1-9. (in Chinese with English abstract)
[4]	叶恭银, 方琦, 徐红星, 等. 我国水稻螟虫发生及治理研究进展[J]. 植物保护, 2023, 49(5): 167-180.
	YE G Y, FANG Q, XU H X, et al. Research advances on the occurrence, damage and management of rice stem borers in China[J]. Plant Protection, 2023, 49(5): 167-180. (in Chinese with English abstract)
[5]	中华人民共和国农业农村部. 一类农作物病虫害名录[EB/OL]. 北京:(2023-03-07)[2023-07-18]. http://www.moa.gov.cn/govpublic//ZZYGLS/202303/t20230314_6422981.htm.
[6]	盛承发, 宣维健, 焦晓国, 等. 我国稻螟暴发成灾的原因、趋势及对策[J]. 自然灾害学报, 2002, 11(3): 103-108.
	SHENG C F, XUAN W J, JIAO X G, et al. Causes, trend and control strategies of disaster of rice borers in China[J]. Journal of Natural Disasters, 2002, 11(3): 103-108. (in Chinese with English abstract)
[7]	盛承发, 王红托, 盛世余, 等. 我国稻螟灾害的现状及损失估计[J]. 昆虫知识, 2003, 40(4): 289-294.
	SHENG C F, WANG H T, SHENG S Y, et al. Pest status and loss assessment of crop damage caused by the rice borers, Chilo suppressalis and Tryporyza incertulas in China[J]. Entomological Knowledge, 2003, 40(4): 289-294. (in Chinese with English abstract)
[8]	XU H X, YE X H, YANG Y J, et al. Comparative genomics sheds light on the convergent evolution of miniaturized wasps[J]. Molecular Biology and Evolution, 2021, 38(12): 5539-5554.
[9]	TANG R, BABENDREIER D, ZHANG F, et al. Assessment of Trichogramma japonicum and T. chilonis as potential biological control agents of yellow stem borer in rice[J]. Insects, 2017, 8(1): 19.
[10]	LU Y H, ZHAO Y Y, LU H, et al. Midgut transcriptional variation of Chilo suppressalis larvae induced by feeding on the dead-end trap plant, Vetiveria zizanioides[J]. Frontiers in Physiology, 2018, 9: 1067.
[11]	LU Y H, BAI Q, LI Q, et al. Two P450 genes, CYP6SN3 and CYP306A1 involved in the growth and development of Chilo suppressalis and the lethal effect caused by vetiver grass[J]. International Journal of Biological Macromolecules, 2022, 223: 860-869.
[12]	DEKA S, BARTHAKUR S. Overview on current status of biotechnological interventions on yellow stem borer Scirpophaga incertulas(Lepidoptera: Crambidae) resistance in rice[J]. Biotechnology Advances, 2010, 28(1): 70-81.
[13]	TU J, ZHANG G, DATTA K, et al. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis delta-endotoxin[J]. Nature Biotechnology, 2000, 18(10): 1101-1104.
[14]	顾正远, 肖英方, 王益民. 江苏省主要水稻品种对三种害虫的抗性[J]. 江苏农业学报, 1989, 5(2): 38-41.
	GU Z Y, XIAO Y F, WANG Y M. Resistance of main rice varieties or lines to brown planthopper, whitebacked planthopper and striped stem borer in Jiangsu[J]. Jiangsu Journal of Agricultural Sciences, 1989, 5(2): 38-41. (in Chinese with English abstract)
[15]	束兆林, 方继朝, 盛生兰, 等. 水稻品种(系)对二化螟抗性的初步研究[J]. 华东昆虫学报, 2003, 12(1): 14-18.
	SHU Z L, FANG J C, SHENG S L, et al. Study on resistance of the rice varieties to Chilo suppressalis(Walker)[J]. Entomological Journal of East China, 2003, 12(1): 14-18. (in Chinese with English abstract)
[16]	徐红星, 吕仲贤, 陈建明, 等. 不同水稻品种对二化螟的抗性及其与形态学和解剖学特征的关系[J]. 植物保护学报, 2006, 33(3): 241-245.
	XU H X, LV Z X, CHEN J M, et al. Resistance of different rice varieties to the striped stem borer, Chilo suppressalis, and its relationship with the morphological and anatomic characteristics of rice[J]. Journal of Plant Protection, 2006, 33(3): 241-245. (in Chinese with English abstract)
[17]	秦文婧, 黄水金, 黄建华, 等. 抗二化螟的水稻品种筛选[J]. 应用昆虫学报, 2015, 52(3): 721-727.
	QIN W J, HUANG S J, HUANG J H, et al. Resistance of rice varieties to the stripe stem borer Chilo suppressalis[J]. Chinese Journal of Applied Entomology, 2015, 52(3): 721-727. (in Chinese with English abstract)
[18]	SELVI A, SHANMUGASUNDARAM P, MOHAN KUMAR S, et al. Molecular markers for yellow stem borer Scirpophaga incertulas(Walker) resistance in rice[J]. Euphytica, 2002, 124(3): 371-377.
[19]	林贤文. 水稻抗螟虫及其相关性状的数量遗传分析与验证[D]. 杭州: 浙江大学, 2010.
	LIN X W. Genetic analysis and validation of the quantitative resistance and its related traits of rice to the stem borerp[D]. Hangzhou: Zhejiang University, 2010. (in Chinese with English abstract)
[20]	ZHU W Y, LIN J, YANG D W, et al. Development of chromosome segment substitution lines derived from backcross between two sequenced rice cultivars, indica recipient 93-11 and Japonica donor nipponbare[J]. Plant Molecular Biology Reporter, 2009, 27(2): 126-131.
[21]	ZHANG H, ZHAO Q, SUN Z Z, et al. Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of Japonica Nipponbare[J]. Journal of Genetics and Genomics, 2011, 38(12): 603-611.
[22]	BROMAN K W, WU H, SEN S, et al. R/QTL: QTL mapping in experimental crosses[J]. Bioinformatics, 2003, 19(7): 889-890.
[23]	HALEY C S, KNOTT S A. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers[J]. Heredity, 1992, 69(4): 315-324.

利用染色体片段替换系鉴定水稻二化螟抗性QTL

Identification of QTLs for stem borer resistance using chromosome segment substitution lines in rice

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