镉胁迫对不同甜玉米自交系幼苗生长的影响及其相关简单重复序列分子标记初筛

doi:10.3969/j.issn.1004-1524.2022.08.02

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (8): 1582-1590.DOI: 10.3969/j.issn.1004-1524.2022.08.02

镉胁迫对不同甜玉米自交系幼苗生长的影响及其相关简单重复序列分子标记初筛

姜昊梁¹^,²(), 黄允¹, 梁绍芳¹^,², 谢梦晨¹^,², 徐天成¹, 宋芷婷¹^,², 向文文¹^,², 陈青春¹^,², 万小荣¹^,², 孙伟¹^,²^,^*()

1.仲恺农业工程学院农业与生物学院,广东广州 510225
2.广州市特色作物种质资源研究与利用重点实验室,广东广州 510225

收稿日期:2021-09-21 出版日期:2022-08-25 发布日期:2022-08-26
通讯作者: 孙伟
作者简介:*孙伟,E-mail: starking521@126.com
姜昊梁(1994—),男,山东烟台人,硕士研究生,主要从事玉米抗逆分子遗传育种相关研究。E-mail: jianghaoliang094@163.com
基金资助:
国家自然科学基金(31901565);广州市特色作物种质资源研究与利用重点实验室项目(202002010010)

Influences of cadmium stress on seedling growth of different sweet corn inbred lines and screening of associated molecular markers via simple sequence repeats

JIANG Haoliang¹^,²(), HUANG Yun¹, LIANG Shaofang¹^,², XIE Mengchen¹^,², XU Tiancheng¹, SONG Zhiting¹^,², XIANG Wenwen¹^,², CHEN Qingchun¹^,², WAN Xiaorong¹^,², SUN Wei¹^,²^,^*()

1. College of Agriculture & Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
2. Guangzhou Key Laboratory for Research and Development of Crop Germplasm Resources, Guangzhou 510225, China

Received:2021-09-21 Online:2022-08-25 Published:2022-08-26
Contact: SUN Wei

摘要/Abstract

摘要：

为探究不同甜玉米自交系幼苗期对镉的响应差异,发掘与甜玉米苗期耐镉性状相关的分子标记位点,选取13份甜玉米自交系进行镉胁迫处理(Cd浓度为0.01 mol·L^-1),综合评价甜玉米幼苗生理指标差异,并寻找与甜玉米苗期耐镉性相关的简单重复序列(SSR)分子标记。结果显示,从耐镉性判断,KY188、M114A为敏感型自交系,T96-4、M3、T35和T8-1为耐受型自交系。与对照处理相比:KY188(敏感型)经过镉胁迫处理后,叶绿素含量随时间推进显著(P<0.05)降低,苗高、总根数、根长、地上部鲜重和干重、地下部鲜重和干重、总鲜重、总干重分别显著(P<0.05)降低了18.87%、24.32%、21.13%、58.86%、2.04%、33.77%、26.11%、47.11%、14.29%;T96-4(耐受型)经过镉胁迫处理后,上述指标无显著变化。通过相关性分析,共检测到9个与甜玉米苗期耐镉性状相关的SSR标记,分别位于第2、3、4、5、6、8、9、10号染色体上。

关键词: 镉胁迫, 主成分分析, 耐镉性, 简单重复序列分子标记, 甜玉米

Abstract:

In order to explore the responses of different sweet corn inbred lines to cadmium at seedling stage and to screen molecular markers associated with these responses, 13 sweet corn inbred lines were treated with 0.01 mol·L^-1 cadmium, comprehensive evaluation of these sweet corn inbred lines was carried out based on physiological indexes, and the associated molecular makers were identified via genomic simple sequence repeats (SSR) markers. It was shown that KY188 and M114A were sensitive materials, and T96-4, M3, T35 and T8-1 were tolerant materials by Cd tolerance analysis. Compared with the control (without Cd), the chlorophyll content of KY188 (Cd sensitive inbred line) treated with Cd significantly (P<0.05) decreased with time, and its plant height, root number, root length, fresh weight of shoot, dry weight of shoot, fresh weight of root, dry weight of root, total fresh weight of plant, total dry weight of plant were significantly (P<0.05) decreased by 18.87%, 24.32%, 21.13%, 58.86%, 2.04%, 33.77%, 26.11%, 47.11% and 14.29%, respectively. However, the tolerant inbred line T96-4 showed no significant changes in the above indexes after Cd treatment. Through correlation analysis, 9 SSR markers were screened out on the 2nd, 3rd, 4th, 5th, 6th, 8th, 9th and 10th chromosome.

Key words: cadmium stress, principal component analysis, cadmium tolerance, simple sequence repeats (SSR) molecular markers, sweet corn

中图分类号:

S332.1
S513

姜昊梁, 黄允, 梁绍芳, 谢梦晨, 徐天成, 宋芷婷, 向文文, 陈青春, 万小荣, 孙伟. 镉胁迫对不同甜玉米自交系幼苗生长的影响及其相关简单重复序列分子标记初筛[J]. 浙江农业学报, 2022, 34(8): 1582-1590.

JIANG Haoliang, HUANG Yun, LIANG Shaofang, XIE Mengchen, XU Tiancheng, SONG Zhiting, XIANG Wenwen, CHEN Qingchun, WAN Xiaorong, SUN Wei. Influences of cadmium stress on seedling growth of different sweet corn inbred lines and screening of associated molecular markers via simple sequence repeats[J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1582-1590.

图/表 7

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性状Trait	RPH	RRL	RRN	RSFW	RRFW	RSDW	RRDW	RSFW/RRFW	RSDW/RRDW	RTDW
RRL	0.431^**
RRN	0.586^**	0.274^*
RSFW	0.845^**	0.230	0.601^**
RRFW	0.659^**	-0.009	0.503^**	0.855^**
RSDW	0.743^**	0.484^**	0.810^**	0.788^**	0.634^**
RRDW	0.087	-0.104	0.122	0.129	0.198	0.102
RSFW/RRFW	0.039	0.450^**	0.443^**	-0.071	-0.048	0.526^**	-0.027
RSDW/RRDW	-0.113	-0.125	-0.036	-0.109	-0.082	-0.103	0.954^**	-0.048
RTDW	0.371^**	-0.069	0.325^*	0.480^**	0.564^**	0.368^**	0.910^**	-0.046	0.762^**
RTFW	0.828^**	0.330^*	0.733^**	0.944^**	0.825^**	0.918^**	0.136	0.196	-0.111	0.470^**

性状Trait	RPH	RRL	RRN	RSFW	RRFW	RSDW	RRDW	RSFW/RRFW	RSDW/RRDW	RTDW
RRL	0.431^**
RRN	0.586^**	0.274^*
RSFW	0.845^**	0.230	0.601^**
RRFW	0.659^**	-0.009	0.503^**	0.855^**
RSDW	0.743^**	0.484^**	0.810^**	0.788^**	0.634^**
RRDW	0.087	-0.104	0.122	0.129	0.198	0.102
RSFW/RRFW	0.039	0.450^**	0.443^**	-0.071	-0.048	0.526^**	-0.027
RSDW/RRDW	-0.113	-0.125	-0.036	-0.109	-0.082	-0.103	0.954^**	-0.048
RTDW	0.371^**	-0.069	0.325^*	0.480^**	0.564^**	0.368^**	0.910^**	-0.046	0.762^**
RTFW	0.828^**	0.330^*	0.733^**	0.944^**	0.825^**	0.918^**	0.136	0.196	-0.111	0.470^**

自交系Inbred lines	CI₁	CI₂	CI₃	U₁	U₂	U₃	D	耐镉性Cd tolerance
KY188	-2.551	-3.822	1.704	0	0	0.975	0.099	不耐镉Not tolerant
M114A	-1.579	-1.487	-2.335	0.233	0.485	0	0.230	不耐镉Not tolerant
M103	-0.795	-0.881	-1.066	0.420	0.611	0.306	0.377	中度耐镉Medium tolerant
M119	-0.554	-0.537	-1.190	0.478	0.682	0.276	0.418	中度耐镉Medium tolerant
T52-1	-0.203	-0.418	-0.575	0.562	0.707	0.425	0.477	中度耐镉Medium tolerant
M118	-0.976	0.623	0.505	0.377	0.923	0.686	0.478	中度耐镉Medium tolerant
T96-2	-0.396	-0.148	-0.275	0.516	0.763	0.497	0.478	中度耐镉Medium tolerant
M55	-0.083	-0.205	-1.351	0.591	0.751	0.238	0.482	中度耐镉Medium tolerant
T43	-0.283	-0.205	-0.340	0.543	0.751	0.482	0.486	中度耐镉Medium tolerant
T35	0.148	0.152	0.227	0.646	0.826	0.618	0.564	高度耐镉Highly tolerant
T8-1	0.707	0.055	1.807	0.780	0.805	1.000	0.657	高度耐镉Highly tolerant
M3	1.297	0.372	-0.388	0.921	0.871	0.470	0.683	高度耐镉Highly tolerant
T96-4	1.628	0.992	-2.241	1.000	1.000	0.023	0.706	高度耐镉Highly tolerant

自交系Inbred lines	CI₁	CI₂	CI₃	U₁	U₂	U₃	D	耐镉性Cd tolerance
KY188	-2.551	-3.822	1.704	0	0	0.975	0.099	不耐镉Not tolerant
M114A	-1.579	-1.487	-2.335	0.233	0.485	0	0.230	不耐镉Not tolerant
M103	-0.795	-0.881	-1.066	0.420	0.611	0.306	0.377	中度耐镉Medium tolerant
M119	-0.554	-0.537	-1.190	0.478	0.682	0.276	0.418	中度耐镉Medium tolerant
T52-1	-0.203	-0.418	-0.575	0.562	0.707	0.425	0.477	中度耐镉Medium tolerant
M118	-0.976	0.623	0.505	0.377	0.923	0.686	0.478	中度耐镉Medium tolerant
T96-2	-0.396	-0.148	-0.275	0.516	0.763	0.497	0.478	中度耐镉Medium tolerant
M55	-0.083	-0.205	-1.351	0.591	0.751	0.238	0.482	中度耐镉Medium tolerant
T43	-0.283	-0.205	-0.340	0.543	0.751	0.482	0.486	中度耐镉Medium tolerant
T35	0.148	0.152	0.227	0.646	0.826	0.618	0.564	高度耐镉Highly tolerant
T8-1	0.707	0.055	1.807	0.780	0.805	1.000	0.657	高度耐镉Highly tolerant
M3	1.297	0.372	-0.388	0.921	0.871	0.470	0.683	高度耐镉Highly tolerant
T96-4	1.628	0.992	-2.241	1.000	1.000	0.023	0.706	高度耐镉Highly tolerant

自交系Inbred lines	处理Treatment	苗高Plant height/cm	总根数Root number	根长Root length/cm
KY188	对照Control	14.52±0.54 a	9.17±0.85 a	14.01±1.19 a
	镉胁迫Cd stress	11.78±0.61 b	6.94±0.49 b	11.05±0.64 b
T96-4	对照Control	14.87±0.61 a	7.17±0.45 a	13.45±0.89 a
	镉胁迫Cd stress	14.77±0.65 a	7.63±0.49 a	13.29±1.08 a

镉胁迫对不同甜玉米自交系幼苗生长的影响及其相关简单重复序列分子标记初筛

Influences of cadmium stress on seedling growth of different sweet corn inbred lines and screening of associated molecular markers via simple sequence repeats

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标记名称Marker name	染色体Chromosome	引物序列Primer sequences(5'→3')	P
bnlg108	2	F: GCACTCACGCGCACAGGTCA R:CGCCTGCCAAGGTACATCAC	0.001 3
bnlg1456	3	F: CTCTAGGTGGTTAAGATTAACTC R: TTCATGAGGACCGTGTTGAA	0.045 0
bnlg589	4	F: GGGTCGTTTAGGGAGGCACCTTTG R: GCGACAGACAGACAGACAAGCG	0.023 0
bnlg2305	5	F: CACCTTGAAAGCATCCTCGT R: GTATCACACCCTCTGCTGCA	0.000 2
umc1083	6	F: CTTTCCTCTCTGGAGCGTGTATTG R: ATATGTTGCAGAACCATCCAGGTC	0.005 6
umc1139	8	F: TTTGTAATATGGCGCTCGAAAACT R: GAAGACGCCTCCAAGATGGATAC	0.000 4
umc1170	9	F: TGGGTGCTAAAACGTAACAACAAA R: GAGGACGAAGCAGAAATCCTACC	0.004 2
bnlg210	10	F: GCCTCGCACCAAGACATAATA R: TGCCCCATTTGAGTAGACTTC	0.000 8
phi059	10	F: AAGCTAATTAAGGCCGGTCATCCC R: TCCGTGTACTCGGCGGACTC	0.000 2

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