浙江农业学报 ›› 2019, Vol. 31 ›› Issue (9): 1531-1540.DOI: 10.3969/j.issn.1004-1524.2019.09.18

• 环境科学 • 上一篇    下一篇

基于主成分分析及隶属函数法的巨菌草幼苗耐盐碱性评价

闫晶秋子1, 李钢铁1,*, 刘玉军2, 麻云霞1, 杨颖1   

  1. 1.内蒙古农业大学 沙漠治理学院,内蒙古 呼和浩特 010018;
    2.通辽市奈曼旗林业局兴隆沼林场,内蒙古 通辽 028300
  • 收稿日期:2019-04-01 出版日期:2019-09-25 发布日期:2019-10-11
  • 通讯作者: *李钢铁,E-mail: 13848817183@163.com
  • 作者简介:闫晶秋子(1994—),女,甘肃金塔人,硕士研究生,研究方向为沙区植物资源保护与利用。E-mail:450604975@qq.com
  • 基金资助:
    内蒙古自治区科技成果转化项目(CGZH2018137)

Evaluation of salt and alkali resistance of Pennisetum giganteum Z. X. Lin seedlings based on principal component analysis and membership function method

YAN Jingqiuzi1, LI Gangtie1,*, LIU Yujun2, MA Yunxia1, YANG Ying1   

  1. 1. College of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Xinglongzhao Forest Farm, Naimanqi Forestry Bureau of Tongliao City, Tongliao 028300, China
  • Received:2019-04-01 Online:2019-09-25 Published:2019-10-11

摘要: 模拟不同浓度的混合盐碱胁迫环境,测定巨菌草幼苗的株高生长量(H)、含水量(MC)、相对含水量(RWC)、水分饱和亏(WSD)、丙二醛含量(MDA)、过氧化氢酶活性(CAT)、超氧化物歧化酶活性(SOD)、可溶性糖含量(SS)、游离脯氨酸含量(Pro)、可溶性蛋白含量(SP)、叶绿素含量(Chl)(含叶绿素a含量Chla、叶绿素b含量Chlb)的变化,对其抗盐碱性进行综合评价。结果表明:随盐碱浓度升高,不同混合盐碱胁迫下巨菌草幼苗的H、RWC不同程度地下降,CAT、SOD活性,以及SS、SP、Pro含量变化不一。当组配的溶液中NaCl、Na2SO4、NaHCO3浓度比为1:2:1、总盐碱浓度为30 mmol·L-1时,供试巨菌草的隶属函数综合得分最高,为0.893,说明巨菌草在该环境下耐受性较好。

关键词: 巨菌草, 幼苗耐盐性, 主成分分析, 隶属函数

Abstract: The plant height increase (H), water content (MC), relative water content (RWC), water saturation deficit (WSD), malondialdehyde content (MDA), catalase activity (CAT), superoxide dismutase activity (SOD), soluble sugar content (SS), free proline content (Pro), soluble protein content (SP), chlorophyll content (Chl) (including chlorophyll a content Chla, and chlorophyll b content Chlb) of Pennisetum giganteum Z. X. Lin seedlings were determined under simulated saline-alkali stress, and comprehensive evaluation of saline-alkali resistance was also carried out. It was shown that with the increase of saline-alkali concentration, H, RWC decreased, while the activities of CAT, SOD, and contents of SS, SP, Pro varied differently. When the concentration of test solution was 30 mmol·L-1, and the ratio of NaCl, Na2SO4, NaHCO3 concentration was 1:2:1, the comprehensive score of membership function was the highest (0.893), indicating good tolerance of Pennisetum giganteum Z. X. Lin under this simulated environment.

Key words: Pennisetum giganteum Z. X. Lin, salt tolerance of seedlings, principal component analysis, membership function

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