五个抗稻瘟病基因在浙江省水稻品种中的分布和抗性评价

doi:10.3969/j.issn.1004-1524.2019.06.09

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (6): 922-929.DOI: 10.3969/j.issn.1004-1524.2019.06.09

五个抗稻瘟病基因在浙江省水稻品种中的分布和抗性评价

何海燕^{1, 2, 3}, 柴荣耀^{2, 3}, 邱海萍^{2, 3}, 毛雪琴^{2, 3}, 王艳丽^{2, 3}, 孙国仓^{2, 3, *}

1.中国农业科学院研究生院,北京 100080;
2.浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021;
3.浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地,浙江杭州 310021;

收稿日期:2018-11-13 出版日期:2019-06-25 发布日期:2019-06-26
通讯作者: 孙国仓,E-mail:sungc01@sina. com
作者简介:何海燕(1981—),女,浙江江山人,硕士,助理研究员,主要从事水稻抗病育种研究。E-mail: hehaiyan@zaas.ac.cn
基金资助:
国家自然科学基金(31470249); 国家重点研发计划(2016YFD0300706,2016YFD0200804); 浙江省“三农六方”科技协作项目(CTZB-F160728AWZ-SNY1-6); 浙江省重点研发计划(2016C02050-3); 湖南省农业科学院科技创新项目(2017JC08)

Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang

HE Haiyan^{1, 2, 3}, CHAI Rongyao^{2, 3}, QIU Haiping^{2, 3}, MAO Xueqin^{2, 3}, WANG Yanli^{2, 3}, SUN Guocang^{2, 3, *}

1. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100080,China;
2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China;
3. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Hangzhou 310021,China

Received:2018-11-13 Online:2019-06-25 Published:2019-06-26

摘要/Abstract

摘要： 为了明确浙江省部分水稻主栽品种和中国稻瘟菌生理小种鉴别品种中稻瘟病抗性基因分布情况,本研究采用5个基于稻瘟病抗感等位基因序列设计的功能性分子标记,对40份浙江省水稻主栽品种和6份稻瘟菌鉴别品种中Pit、Pib、Pii/Pi3/Pi5、Pia、Pi1等5个稻瘟病抗性基因进行了分子检测。结果发现,这5个抗性基因在浙江省栽培品种中分布频率不同,其中Pib基因分布最广,占63.04%;其次是Pia,占58.70%;携带Pii/Pi3/Pi5和Pit抗性基因的材料较少,分别为21.74%和10.87%。同时用2015—2017年田间采集到的141个稻瘟菌菌株检测46个水稻品种苗瘟抗性水平,结果显示,其中仅15个品种的抗性频率在70%以上,大部分品种携带其中1~2个抗性基因。聚合了多个抗性基因的品种抗性水平相对较高。

关键词: 水稻, 稻瘟病, 抗病基因, 分子标记

Abstract: In order to clarify the distribution of blast resistance genes in some rice cultivars and blast-resistant cultivars in Zhejiang Province, five functional molecular markers based on blast resistance allele sequences were used to detect Pit, Pib, Pii/Pi3/Pi5, Pia and Pi1 resistance genes in 40 main rice varieties in Zhejiang Province and 6 Chinese rice blast identification varieties. The results showed that the distribution frequencies of the five resistance genes were different, Pib gene was most widely distributed, accounting for 63.04%, followed by Pia, accounting for 58.70%, Pii/Pi3/Pi5 and Pit resistance genes were less, accounting for 21.74% and 10.87% respectively. At the same time, the resistance level of 46 rice varieties to Magnaporthe grisea was detected by 141 strains collected in the field from 2015 to 2017. The results showed that the resistance frequency of 15 varieties was more than 70%. Most of the varieties carried one or two resistance genes. The resistance level of varieties with multiple resistance genes was relatively higher.

Key words: rice, rice leaf blast, resistance gene, molecular marker

中图分类号:

S435.111.4⁺1

何海燕, 柴荣耀, 邱海萍, 毛雪琴, 王艳丽, 孙国仓. 五个抗稻瘟病基因在浙江省水稻品种中的分布和抗性评价[J]. 浙江农业学报, 2019, 31(6): 922-929.

HE Haiyan, CHAI Rongyao, QIU Haiping, MAO Xueqin, WANG Yanli, SUN Guocang. Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang[J]. , 2019, 31(6): 922-929.

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五个抗稻瘟病基因在浙江省水稻品种中的分布和抗性评价

Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang

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