关键词: 浙贝母, SCoT分子标记, 遗传多样性, 聚类分析, 相关性分析

Abstract:

To provide theoretical support for germplasm resources evaluation, new varieties development and breeding of Fritillaria thunbergii in Zhangshui, Ningbo, SCoT molecular marker technique was used in this study, genetic diversity of 146 germplasm resources of Fritillaria thunbergii in Zhangshui, Ningbo were analyzed; then the correlation between phenotypes of germplasm resources and the correlation between phenotypic traits and alkaloid content of Fritillary thunbergii by using Pearson’s correlation analysis. The results indicated that total of 111 sites were amplified from 5 SCoT primers, including 96 polymorphic sites, and the average ratio of polymorphic sites was 86.29%. In the genetic diversity analysis at the overall sample level, Na, Ne, H and I were 2.000, 1.585 8, 0.340 5 and 0.509 8 respectively, the results showed that the polymorphism of the primers was high, which could effectively reveal the diversity of germplasm resources of 146 Fritillaria thunbergii. The cluster analysis showed that the genetic similarity coefficient between different Fritillaria thunbergii germplasm resources was between 0.55 and 1.00, the characteristics of offspring were mainly in herited from their parents in two treatments of systematic selection and seed irradiation. The genetic relationship and phenotypic traits of the offspring after different hybridization treatment groups were basically biased on the paternal parent, which was in conformity with the investigation results of germplasm resource traits. The results of Pearson’s correlation analysis showed that there was significantly positive correlation between flower type and leaf number, flower color and leaf number, leaf color and seed setting degree were significantly positively correlated, flower type and leaf color; the inner wall color of flowers was negatively correlated with the number of leaves, flower type and flower color with stem thickness and seed setting degree. Meanwhile, the leaf color and flower color, leaf width and seed setting degree, flower type and seed setting degree were negatively correlated; and leaf width was negatively correlated with peimine, peiminine and total alkaloids. To sum up, SCoT molecular marker technique was used to reveal the genetic diversity of germplasm resources of Fritillaria thunbergii in Zhangshui, Ningbo, it can be effectively used for the superior breeding of Fritillaria thunbergii germplasm resources and reference for the molecular assisted breeding, development and utilization of germplasm resources.

Key words: Fritillaria thunbergii, SCoT molecular marker, genetic diversity, cluster analysis, correlation analysis