浙江农业学报 ›› 2026, Vol. 38 ›› Issue (4): 755-768.DOI: 10.3969/j.issn.1004-1524.20250325
叶慎奕1,2(
), 沈超怡2, 牛犇2, 刘瑞玲2, 房祥军2, 陈慧芝2, 陈杭君2, 吴伟杰3,*(
), 郜海燕2,*(
)
收稿日期:2025-04-22
出版日期:2026-04-25
发布日期:2026-05-08
作者简介:郜海燕,E-mail:spsghy@163.com通讯作者:
吴伟杰,郜海燕
基金资助:
YE Shenyi1,2(
), SHEN Chaoyi2, NIU Ben2, LIU Ruiling2, FANG Xiangjun2, CHEN Huizhi2, CHEN Hangjun2, WU Weijie3,*(
), GAO Haiyan2,*(
)
Received:2025-04-22
Published:2026-04-25
Online:2026-05-08
Contact:
WU Weijie,GAO Haiyan
摘要:
为探究浙江、四川、江西和安徽4个产地蓝美1号蓝莓果实的营养与品质差异,本研究通过代谢组学分析与营养指标测定,比较了不同产地果实的代谢物组成,并选取四川产地的蓝莓进行加工适用性研究。共鉴定出2 167种代谢物。与其他产地相比,浙江产地蓝莓的显著差异代谢物数量较多,富含有机酸、酯类、类黄酮及其衍生物等特有成分;四川和安徽产地蓝莓差异代谢物较为丰富,含有较多类黄酮和有机氧化合物;江西产地蓝莓的有机氧化合物含量最高,但类黄酮与花色苷独特性较低。代谢通路分析表明,与浙江产地相比,四川和江西产地蓝莓的代谢物富集于氨基酸和亚油酸代谢通路,安徽产地蓝莓则富集于黄酮类生物合成通路。四川蓝莓因维生素C和可溶性糖含量较高,相较于鲜食更适合加工。对其基础加工产品的评价显示,果脯能较好地保留营养物质,果汁工艺有待优化。四川产地蓝莓较适宜用于功能食品等深度加工,以充分发挥其营养优势。本研究获得的代谢组数据可为蓝莓原料选择与加工应用提供科学依据。
中图分类号:
叶慎奕, 沈超怡, 牛犇, 刘瑞玲, 房祥军, 陈慧芝, 陈杭君, 吴伟杰, 郜海燕. 蓝莓原料产地差异及其加工适用性[J]. 浙江农业学报, 2026, 38(4): 755-768.
YE Shenyi, SHEN Chaoyi, NIU Ben, LIU Ruiling, FANG Xiangjun, CHEN Huizhi, CHEN Hangjun, WU Weijie, GAO Haiyan. Geographic origin variation of blueberry raw materials and their processing suitability[J]. Acta Agriculturae Zhejiangensis, 2026, 38(4): 755-768.
| 项目 Items | 评分依据 Scoring basis | 评分标准 Scoring criteria |
|---|---|---|
| 组织形态(25分) Texture (25 score) | 果脯形态完整,皱缩程度适中;果酱黏稠不流散,不析水且无杂质;果汁液体均匀无沉淀 Preserved fruit: intact shape, moderate shrinkage; jam: thick, no separation or impurities; juice: uniform, no sediment | 15~25 |
| 果脯形态较完整,略有皱缩;果酱黏稠程度中等,析水程度及杂质较少;果汁液体均匀, 有少许沉淀 Preserved fruit: mostly intact, slight shrinkage; jam: moderately thick, slight separation or impurities; juice: mostly uniform, slight sediment | 5~14 | |
| 果脯形态不完整或残缺,皱缩严重;果酱分层且析水较多;果汁液体有分层或沉淀情况产生 Preserved fruit: broken/severe shrinkage; jam: watery, noticeable separation; juice: layered or significant sediment | 0~4 | |
| 色泽(25分) Color(25 score) | 果脯色泽深蓝紫色,表面鲜亮有光泽感;果酱呈深蓝紫色,鲜亮光泽;果汁类深红色,色泽鲜亮 Preserved fruit: deep blue-purple color, bright and glossy surface; jam: deep blue-purple, vivid and shiny; juice: deep red, bright color | 15~25 |
| 果脯色泽较深,光泽中等;果酱色泽和光泽中等;果汁亮红色,色泽较亮 Preserved fruit: relatively dark color, moderate gloss; jam: medium color and gloss; juice: bright red, relatively vivid color | 5~14 | |
| 果脯色泽浅且暗淡,无光泽;果酱色泽发暗或发灰,无光泽;果汁红色,色泽暗淡 Preserved fruit: pale and dull color, no gloss; jam: dark or grayish color, no gloss; juice: red, dull color | 0~4 | |
| 气味(25分) | 有明显蓝莓特有香味,且气味纯正Distinct pure blueberry aroma | 15~25 |
| Aroma(25 score) | 蓝莓特有香气较明显Moderate blueberry aroma | 5~14 |
| 蓝莓特有气味不明显或有明显怪味Faint blueberry aroma or noticeable off-flavor | 0~4 | |
| 口感(25分) Taste(25 score) | 果脯和果酱酸甜可口,有果肉感;果汁口感顺滑细腻,酸甜适中 Preserved fruit and jam: balanced sweet-sour taste with good fruit pulp texture; juice: smooth, fine texture with well-balanced sweetness and acidity | 15~25 |
| 口感中等,稍偏酸或偏甜Slightly skewed sweetness or sourness | 5~14 | |
| 口味过淡或不适,较明显偏酸或偏甜Bland or unpleasant taste; pronounced imbalance | 0~4 |
表1 蓝莓产品感官评价标准
Table 1 Sensory evaluation criteria for blueberry products
| 项目 Items | 评分依据 Scoring basis | 评分标准 Scoring criteria |
|---|---|---|
| 组织形态(25分) Texture (25 score) | 果脯形态完整,皱缩程度适中;果酱黏稠不流散,不析水且无杂质;果汁液体均匀无沉淀 Preserved fruit: intact shape, moderate shrinkage; jam: thick, no separation or impurities; juice: uniform, no sediment | 15~25 |
| 果脯形态较完整,略有皱缩;果酱黏稠程度中等,析水程度及杂质较少;果汁液体均匀, 有少许沉淀 Preserved fruit: mostly intact, slight shrinkage; jam: moderately thick, slight separation or impurities; juice: mostly uniform, slight sediment | 5~14 | |
| 果脯形态不完整或残缺,皱缩严重;果酱分层且析水较多;果汁液体有分层或沉淀情况产生 Preserved fruit: broken/severe shrinkage; jam: watery, noticeable separation; juice: layered or significant sediment | 0~4 | |
| 色泽(25分) Color(25 score) | 果脯色泽深蓝紫色,表面鲜亮有光泽感;果酱呈深蓝紫色,鲜亮光泽;果汁类深红色,色泽鲜亮 Preserved fruit: deep blue-purple color, bright and glossy surface; jam: deep blue-purple, vivid and shiny; juice: deep red, bright color | 15~25 |
| 果脯色泽较深,光泽中等;果酱色泽和光泽中等;果汁亮红色,色泽较亮 Preserved fruit: relatively dark color, moderate gloss; jam: medium color and gloss; juice: bright red, relatively vivid color | 5~14 | |
| 果脯色泽浅且暗淡,无光泽;果酱色泽发暗或发灰,无光泽;果汁红色,色泽暗淡 Preserved fruit: pale and dull color, no gloss; jam: dark or grayish color, no gloss; juice: red, dull color | 0~4 | |
| 气味(25分) | 有明显蓝莓特有香味,且气味纯正Distinct pure blueberry aroma | 15~25 |
| Aroma(25 score) | 蓝莓特有香气较明显Moderate blueberry aroma | 5~14 |
| 蓝莓特有气味不明显或有明显怪味Faint blueberry aroma or noticeable off-flavor | 0~4 | |
| 口感(25分) Taste(25 score) | 果脯和果酱酸甜可口,有果肉感;果汁口感顺滑细腻,酸甜适中 Preserved fruit and jam: balanced sweet-sour taste with good fruit pulp texture; juice: smooth, fine texture with well-balanced sweetness and acidity | 15~25 |
| 口感中等,稍偏酸或偏甜Slightly skewed sweetness or sourness | 5~14 | |
| 口味过淡或不适,较明显偏酸或偏甜Bland or unpleasant taste; pronounced imbalance | 0~4 |
| 代谢物分类 Metabolite classification | 各产地的代谢物数量 Quantity of metabolites from different production areas | |||
|---|---|---|---|---|
| 浙江 Zhejiang | 四川 Sichuan | 江西 Jiangxi | 安徽 Anhui | |
| 脂质和类脂分子Lipids and lipid molecules | 249 | 226 | 216 | 210 |
| 有机杂环化合物Organic heterocyclic compounds | 78 | 72 | 71 | 69 |
| 有机氧化合物Organic oxygen compound | 146 | 139 | 135 | 134 |
| 有机酸及其衍生物Organic acids and their derivatives | 128 | 121 | 119 | 118 |
| 有机卤素化合物Organic halogen compounds | 1 | 1 | 1 | 1 |
| 有机硫化合物Organic sulfur compounds | 2 | 2 | 2 | 2 |
| 有机氮化合物Organic nitrogen compounds | 20 | 20 | 20 | 20 |
| 碳氢衍生物Hydrocarbon derivatives | 1 | 1 | 1 | 1 |
| 碳氢化合物Hydrocarbon | 2 | 2 | 2 | 2 |
| 生物碱及其衍生物Alkaloids and their derivatives | 4 | 4 | 4 | 4 |
| 木脂素、新木脂素及其相关化合物Lignans, neolignans and related compounds | 19 | 18 | 16 | 17 |
| 核苷、核苷酸和类似物Nucleosides, nucleotides and analogues | 20 | 19 | 18 | 16 |
| 苯类化合物Benzene compounds | 99 | 97 | 99 | 95 |
| 苯丙类和聚酮类Phenylpropenes and polyketones | 210 | 191 | 186 | 181 |
| 其他Other | 1 067 | 1 051 | 1 037 | 1 007 |
表2 4个产地蓝莓果实代谢物数量统计
Table 2 Quantity statistics of metabolites in blueberry fruits from four production areas
| 代谢物分类 Metabolite classification | 各产地的代谢物数量 Quantity of metabolites from different production areas | |||
|---|---|---|---|---|
| 浙江 Zhejiang | 四川 Sichuan | 江西 Jiangxi | 安徽 Anhui | |
| 脂质和类脂分子Lipids and lipid molecules | 249 | 226 | 216 | 210 |
| 有机杂环化合物Organic heterocyclic compounds | 78 | 72 | 71 | 69 |
| 有机氧化合物Organic oxygen compound | 146 | 139 | 135 | 134 |
| 有机酸及其衍生物Organic acids and their derivatives | 128 | 121 | 119 | 118 |
| 有机卤素化合物Organic halogen compounds | 1 | 1 | 1 | 1 |
| 有机硫化合物Organic sulfur compounds | 2 | 2 | 2 | 2 |
| 有机氮化合物Organic nitrogen compounds | 20 | 20 | 20 | 20 |
| 碳氢衍生物Hydrocarbon derivatives | 1 | 1 | 1 | 1 |
| 碳氢化合物Hydrocarbon | 2 | 2 | 2 | 2 |
| 生物碱及其衍生物Alkaloids and their derivatives | 4 | 4 | 4 | 4 |
| 木脂素、新木脂素及其相关化合物Lignans, neolignans and related compounds | 19 | 18 | 16 | 17 |
| 核苷、核苷酸和类似物Nucleosides, nucleotides and analogues | 20 | 19 | 18 | 16 |
| 苯类化合物Benzene compounds | 99 | 97 | 99 | 95 |
| 苯丙类和聚酮类Phenylpropenes and polyketones | 210 | 191 | 186 | 181 |
| 其他Other | 1 067 | 1 051 | 1 037 | 1 007 |
| 代谢物分类 Metabolite type | 各产地的特征差异代谢物数量(代谢物) Quantity of characteristic differental metabolites from different production areas(metabolites) | |||||||
|---|---|---|---|---|---|---|---|---|
| 浙江Zhejiang | 四川Sichuan | 江西Jiangxi | 安徽Anhui | |||||
| 有机酸 Organic acid | 5(3-硫酸咖啡酸**、3-脱氧-D-甘露糖醛酸酯**、枝孢内酯B**、3,5-二叔丁基-4-羟基苯甲酸钠**、青霉酸**) 5(3-Sulfo-caffeic acid; 3-deoxy-D-mannuronate; cladosporin B; sodium 3,5-di-tert-butyl-4-hydroxybenzoate; penicillic acid) | 6(柠檬酸、反式阿头酸) 6(Citric acid; trans-aconitic acid) | 6(柠檬酸、反式阿头酸) 6(Citric acid; trans-aconitic acid) | 8(柠檬酸、反式阿头酸) 8(Citric acid; trans-aconitic acid) | ||||
| 脂类化合物 Lipid compounds | 7[(24E)-3a-乙酰氧基-15a-羟基-23-氧代-7,9(11),24-羊毛抑菌烯-26-脱醛酸**、柑橘素F**] 7[(24E)-3a-Acetoxy-15a-hydroxy-23-oxo-7,9(11),24-lanostadien-26-oic acid**; citrusin F**] | 8 | 12(6β-羟基杨酸** 6β-Hydroxymellein) | 10 | ||||
| 类黄酮及其衍生物 Flavonoids and their derivatives | 21{2',3,4,4',6'-五羟基查尔酮**、L-表儿茶素**、黄芪素**、木犀草苷A**、5,7-二羟基-2-(3-羟基-4,5-二甲氧基苯基)-3-[(2S,3R,4R,5R,6S)-3,4,5-三羟基-6-甲基氧杂-2-基]氧基铬基-4-酮**、顺式二氢槲皮素**、(2S,3S)-紫杉醇-3-葡萄糖苷**、天竺葵素-3-O-β-D-葡萄糖苷**、芍药素-3-O-α-阿拉伯吡喃糖苷**} 21{2',3,4,4',6'-Pentahydroxychalcone**; L-epicatechin**; astragalin**; luteolinoside A**; 5,7-dihydroxy-2-(3-hydroxy-4,5-dimethoxyphenyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxychromen-4-one**; cis-dihydroquercetin**; (2S,3S)-taxifolin-3-glucoside**; pelargonidin-3-O-β-D-glucoside**; peonidin-3-O-α-arabinopyranoside**} | 25[槲皮素、槲皮素3-(6'-乙酰葡糖苷)、槲皮素3-(6'-丙二酰半乳糖苷)、异槲皮糖苷、槲皮素-7-O-硫酸盐] 25[Quercetin; quercetin 3-(6'-acetylglucoside); quercetin 3-(6'-malonylgalactoside); isoquercitrin; quercetin-7-O-sulfate] | 6[槲皮素、槲皮素3-(6'-乙酰葡糖苷)、槲皮素3-(6'-丙二酰半乳糖苷)、异槲皮糖苷、槲皮素-7-O-硫酸盐] 6[Quercetin; quercetin 3-(6'-acetylglucoside); quercetin 3-(6'-malonylgalactoside); isoquercitrin; quercetin-7-O-sulfate] | 26[槲皮素、槲皮素3-(6’-乙酰葡糖苷)、槲皮素3-(6'-丙二酰半乳糖苷)、异槲皮糖苷、槲皮素-7-O-硫酸盐] 26[Quercetin; quercetin 3-(6'-acetylglucoside); quercetin 3-(6'-malonylgalactoside); isoquercitrin; quercetin-7-O-sulfate] | ||||
| 有机氧化合物 Organic oxygen compound | 12(洋蓟素**、苯乙基芸香糖苷**、阿苏莫苷**、L-鼠李糖呋喃糖**) 12(Cynaropicrin**; phenethyl rutinoside**; azumoside**; L-rhamnofuranose**) | 14{L-二氯酸酯、(2S)-2-[[(2R)-2-[(2-乙基-6-氧代-5-丙-2-基-1H-吡嗪-3-羰基)氨基]-4-甲基戊酰基]氨基]丙酸、黄榄糖苷E} 14{L-Dichloramate; (2S)-2-[[(2R)-2-[(2-ethyl-6-oxo-5-propan-2-yl-1H-pyrazine-3-carbonyl)amino]-4-methylpentanoyl]amino]propanoic acid; huanglansu E} | 16{L-二氯酸酯、(2S)-2-[[(2R)-2-[(2-乙基-6-氧代-5-丙-2-基-1H-吡嗪-3-羰基)氨基]-4-甲基戊酰基]氨基]丙酸、黄榄糖苷E} 16{L-Dichloramate; (2S)-2-[[(2R)-2-[(2-ethyl-6-oxo-5-propan-2-yl-1H-pyrazine-3-carbonyl)amino]-4-methylpentanoyl]amino]propanoic acid; huanglansu E} | 12{L-二氯酸酯、(2S)-2-[[(2R)-2-[(2-乙基-6-氧代-5-丙-2-基-1H-吡嗪-3-羰基)氨基]-4-甲基戊酰基]氨基]丙酸、黄榄糖苷E} 12{L-Dichloramate; (2S)-2-[[(2R)-2-[(2-ethyl-6-oxo-5-propan-2-yl-1H-pyrazine-3-carbonyl)amino]-4-methylpentanoyl]amino]propanoic acid; huanglansu E} | ||||
| 有机杂环化合物 Organic heterocyclic compounds | 6(Artonin Q**、4-羟基维替蒽酮**、大蒜硫胺**、硫酸盐9**、曼苏拉霉素B**) 6(Artonin Q**; 4-hydroxyverticillone**; allithiamine**; sulfate 9**; mansouramycin B**) | 5[4-(3-氯苯基)-α-(二苯基甲基)-1-哌嗪乙醇、9-羟基-4-甲氧基补骨脂素9-葡萄糖苷] 5[4-(3-Chlorophenyl)-α-(diphenylmethyl)-1-piperazineethanol; 9-hydroxy-4-methoxypsoralen 9-glucoside] | 6[4-(3-氯苯基)-α-(二苯基甲基)-1-哌嗪乙醇、9-羟基-4-甲氧基补骨脂素9-葡萄糖苷] 6[4-(3-Chlorophenyl)-α-(diphenylmethyl)-1-piperazineethanol; 9-hydroxy-4-methoxypsoralen 9-glucoside] | 6[4-(3-氯苯基)-α-(二苯基甲基)-1-哌嗪乙醇、9-羟基-4-甲氧基补骨脂素9-葡萄糖苷] 6[4-(3-Chlorophenyl)-α-(diphenylmethyl)-1-piperazineethanol; 9-hydroxy-4-methoxypsoralen 9-glucoside] | ||||
| 萜类 Terpenoids | 2(哈氏罗汉松内酯B** Harziandione B) | 3[(-)-紫苏醇** (-)-Perillyl alcohol] | 3 | 6 | ||||
| 有机氨基化合物 Organic amino compound | 3 | 6(L-脯氨酸**、脯氨酰羟脯氨酸、苯丙氨酸) 6(L-Proline**; prolylhydroxyproline; phenylalanine) | 4(脯氨酰羟脯氨酸、 苯丙氨酸) 4(Prolylhydroxyproline; phenylalanine) | 8(脯氨酰羟脯氨酸、苯丙氨酸) 8(Prolylhyd- roxyproline; phenylalanine) | ||||
| 酯类化合物 Ester compounds | 3(硫酸十二烷基**、康瓦洛糖苷**、葡萄糖酸内酯**) 3(Dodecyl sulfate**; convallatoxin**; gluconolactone**) | 1(蕨甾酮Pterosterone) | 1[(10E,12Z)-9-氢过氧十八烷-10,12-二烯酸(10E,12Z)-9-Hydroperoxyoctadeca-10,12-dienoic acid] | 1[(9S,10E,12Z,15Z)-9-氢过氧十八烷-10,12,15-三烯酸(9S,10E,12Z,15Z)-9-Hydroperoxyoctadeca-10,12,15-trienoic acid] | ||||
| 环多醇 Cyclic polyol | — | — | — | 1(纤维醇** Inositol) | ||||
| 核苷酸及其衍生物 Nucleotides and their derivatives | — | — | 1(1-甲基肌苷** 1-Methylinosine) | — | ||||
| 单宁 Tannin | 1(3-O-甲基鞣花酸** 3-O-Methylellagic acid) | 1(3-O-甲基鞣花酸** 3-O-Methylellagic acid) | — | — | ||||
| 芳香族化合物 Aromatic compounds | 7[1,3,6-三羟基-7-甲氧基-2,5-双(3-甲基-2-丁烯基)氧杂蒽酮**、2D-5-O-甲基-2,3,5/4,6-五羟基环己酮**、芦荟苷B**、棘柱泊苷B**、叶酸菌胆苷B2**] 7[1,3,6-Trihydroxy-7-methoxy-2,5-bis(3-methyl-2-butenyl)xanthen-9-one**; 2D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone**; aloesin B**; echinacoside B**; phyllanthussin B2**] | 3 | 2 | 1 | ||||
| 聚酮类Polyketones | — | 1(罗林霉素A** Roritoxin A) | — | — | ||||
| 总计Total | 67 | 73 | 58 | 78 | ||||
表3 4个产地果实中特征差异代谢物数量统计
Table 3 Statistics on the quantity of characteristic differential metabolites in fruits from four production areas
| 代谢物分类 Metabolite type | 各产地的特征差异代谢物数量(代谢物) Quantity of characteristic differental metabolites from different production areas(metabolites) | |||||||
|---|---|---|---|---|---|---|---|---|
| 浙江Zhejiang | 四川Sichuan | 江西Jiangxi | 安徽Anhui | |||||
| 有机酸 Organic acid | 5(3-硫酸咖啡酸**、3-脱氧-D-甘露糖醛酸酯**、枝孢内酯B**、3,5-二叔丁基-4-羟基苯甲酸钠**、青霉酸**) 5(3-Sulfo-caffeic acid; 3-deoxy-D-mannuronate; cladosporin B; sodium 3,5-di-tert-butyl-4-hydroxybenzoate; penicillic acid) | 6(柠檬酸、反式阿头酸) 6(Citric acid; trans-aconitic acid) | 6(柠檬酸、反式阿头酸) 6(Citric acid; trans-aconitic acid) | 8(柠檬酸、反式阿头酸) 8(Citric acid; trans-aconitic acid) | ||||
| 脂类化合物 Lipid compounds | 7[(24E)-3a-乙酰氧基-15a-羟基-23-氧代-7,9(11),24-羊毛抑菌烯-26-脱醛酸**、柑橘素F**] 7[(24E)-3a-Acetoxy-15a-hydroxy-23-oxo-7,9(11),24-lanostadien-26-oic acid**; citrusin F**] | 8 | 12(6β-羟基杨酸** 6β-Hydroxymellein) | 10 | ||||
| 类黄酮及其衍生物 Flavonoids and their derivatives | 21{2',3,4,4',6'-五羟基查尔酮**、L-表儿茶素**、黄芪素**、木犀草苷A**、5,7-二羟基-2-(3-羟基-4,5-二甲氧基苯基)-3-[(2S,3R,4R,5R,6S)-3,4,5-三羟基-6-甲基氧杂-2-基]氧基铬基-4-酮**、顺式二氢槲皮素**、(2S,3S)-紫杉醇-3-葡萄糖苷**、天竺葵素-3-O-β-D-葡萄糖苷**、芍药素-3-O-α-阿拉伯吡喃糖苷**} 21{2',3,4,4',6'-Pentahydroxychalcone**; L-epicatechin**; astragalin**; luteolinoside A**; 5,7-dihydroxy-2-(3-hydroxy-4,5-dimethoxyphenyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxychromen-4-one**; cis-dihydroquercetin**; (2S,3S)-taxifolin-3-glucoside**; pelargonidin-3-O-β-D-glucoside**; peonidin-3-O-α-arabinopyranoside**} | 25[槲皮素、槲皮素3-(6'-乙酰葡糖苷)、槲皮素3-(6'-丙二酰半乳糖苷)、异槲皮糖苷、槲皮素-7-O-硫酸盐] 25[Quercetin; quercetin 3-(6'-acetylglucoside); quercetin 3-(6'-malonylgalactoside); isoquercitrin; quercetin-7-O-sulfate] | 6[槲皮素、槲皮素3-(6'-乙酰葡糖苷)、槲皮素3-(6'-丙二酰半乳糖苷)、异槲皮糖苷、槲皮素-7-O-硫酸盐] 6[Quercetin; quercetin 3-(6'-acetylglucoside); quercetin 3-(6'-malonylgalactoside); isoquercitrin; quercetin-7-O-sulfate] | 26[槲皮素、槲皮素3-(6’-乙酰葡糖苷)、槲皮素3-(6'-丙二酰半乳糖苷)、异槲皮糖苷、槲皮素-7-O-硫酸盐] 26[Quercetin; quercetin 3-(6'-acetylglucoside); quercetin 3-(6'-malonylgalactoside); isoquercitrin; quercetin-7-O-sulfate] | ||||
| 有机氧化合物 Organic oxygen compound | 12(洋蓟素**、苯乙基芸香糖苷**、阿苏莫苷**、L-鼠李糖呋喃糖**) 12(Cynaropicrin**; phenethyl rutinoside**; azumoside**; L-rhamnofuranose**) | 14{L-二氯酸酯、(2S)-2-[[(2R)-2-[(2-乙基-6-氧代-5-丙-2-基-1H-吡嗪-3-羰基)氨基]-4-甲基戊酰基]氨基]丙酸、黄榄糖苷E} 14{L-Dichloramate; (2S)-2-[[(2R)-2-[(2-ethyl-6-oxo-5-propan-2-yl-1H-pyrazine-3-carbonyl)amino]-4-methylpentanoyl]amino]propanoic acid; huanglansu E} | 16{L-二氯酸酯、(2S)-2-[[(2R)-2-[(2-乙基-6-氧代-5-丙-2-基-1H-吡嗪-3-羰基)氨基]-4-甲基戊酰基]氨基]丙酸、黄榄糖苷E} 16{L-Dichloramate; (2S)-2-[[(2R)-2-[(2-ethyl-6-oxo-5-propan-2-yl-1H-pyrazine-3-carbonyl)amino]-4-methylpentanoyl]amino]propanoic acid; huanglansu E} | 12{L-二氯酸酯、(2S)-2-[[(2R)-2-[(2-乙基-6-氧代-5-丙-2-基-1H-吡嗪-3-羰基)氨基]-4-甲基戊酰基]氨基]丙酸、黄榄糖苷E} 12{L-Dichloramate; (2S)-2-[[(2R)-2-[(2-ethyl-6-oxo-5-propan-2-yl-1H-pyrazine-3-carbonyl)amino]-4-methylpentanoyl]amino]propanoic acid; huanglansu E} | ||||
| 有机杂环化合物 Organic heterocyclic compounds | 6(Artonin Q**、4-羟基维替蒽酮**、大蒜硫胺**、硫酸盐9**、曼苏拉霉素B**) 6(Artonin Q**; 4-hydroxyverticillone**; allithiamine**; sulfate 9**; mansouramycin B**) | 5[4-(3-氯苯基)-α-(二苯基甲基)-1-哌嗪乙醇、9-羟基-4-甲氧基补骨脂素9-葡萄糖苷] 5[4-(3-Chlorophenyl)-α-(diphenylmethyl)-1-piperazineethanol; 9-hydroxy-4-methoxypsoralen 9-glucoside] | 6[4-(3-氯苯基)-α-(二苯基甲基)-1-哌嗪乙醇、9-羟基-4-甲氧基补骨脂素9-葡萄糖苷] 6[4-(3-Chlorophenyl)-α-(diphenylmethyl)-1-piperazineethanol; 9-hydroxy-4-methoxypsoralen 9-glucoside] | 6[4-(3-氯苯基)-α-(二苯基甲基)-1-哌嗪乙醇、9-羟基-4-甲氧基补骨脂素9-葡萄糖苷] 6[4-(3-Chlorophenyl)-α-(diphenylmethyl)-1-piperazineethanol; 9-hydroxy-4-methoxypsoralen 9-glucoside] | ||||
| 萜类 Terpenoids | 2(哈氏罗汉松内酯B** Harziandione B) | 3[(-)-紫苏醇** (-)-Perillyl alcohol] | 3 | 6 | ||||
| 有机氨基化合物 Organic amino compound | 3 | 6(L-脯氨酸**、脯氨酰羟脯氨酸、苯丙氨酸) 6(L-Proline**; prolylhydroxyproline; phenylalanine) | 4(脯氨酰羟脯氨酸、 苯丙氨酸) 4(Prolylhydroxyproline; phenylalanine) | 8(脯氨酰羟脯氨酸、苯丙氨酸) 8(Prolylhyd- roxyproline; phenylalanine) | ||||
| 酯类化合物 Ester compounds | 3(硫酸十二烷基**、康瓦洛糖苷**、葡萄糖酸内酯**) 3(Dodecyl sulfate**; convallatoxin**; gluconolactone**) | 1(蕨甾酮Pterosterone) | 1[(10E,12Z)-9-氢过氧十八烷-10,12-二烯酸(10E,12Z)-9-Hydroperoxyoctadeca-10,12-dienoic acid] | 1[(9S,10E,12Z,15Z)-9-氢过氧十八烷-10,12,15-三烯酸(9S,10E,12Z,15Z)-9-Hydroperoxyoctadeca-10,12,15-trienoic acid] | ||||
| 环多醇 Cyclic polyol | — | — | — | 1(纤维醇** Inositol) | ||||
| 核苷酸及其衍生物 Nucleotides and their derivatives | — | — | 1(1-甲基肌苷** 1-Methylinosine) | — | ||||
| 单宁 Tannin | 1(3-O-甲基鞣花酸** 3-O-Methylellagic acid) | 1(3-O-甲基鞣花酸** 3-O-Methylellagic acid) | — | — | ||||
| 芳香族化合物 Aromatic compounds | 7[1,3,6-三羟基-7-甲氧基-2,5-双(3-甲基-2-丁烯基)氧杂蒽酮**、2D-5-O-甲基-2,3,5/4,6-五羟基环己酮**、芦荟苷B**、棘柱泊苷B**、叶酸菌胆苷B2**] 7[1,3,6-Trihydroxy-7-methoxy-2,5-bis(3-methyl-2-butenyl)xanthen-9-one**; 2D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone**; aloesin B**; echinacoside B**; phyllanthussin B2**] | 3 | 2 | 1 | ||||
| 聚酮类Polyketones | — | 1(罗林霉素A** Roritoxin A) | — | — | ||||
| 总计Total | 67 | 73 | 58 | 78 | ||||
图2 不同产地差异代谢物的KEGG富集图 A,四川vs浙江;B,江西vs浙江;C,安徽vs浙江。
Fig.2 KEGG enrichment map of differential metabolites in different regions A, Sichuan vs Zhejiang; B, Jiangxi vs Zhejiang; C, Anhui vs Zhejiang.
图3 不同产地蓝美1号的品质指标 柱上无相同字母的表示差异显著(p<0.05)。下同。
Fig.3 Quality indicators of Lanmei 1 from different production areas Bars marked without the same letter indicate significant difference at p<0.05.The same as below.
图4 四川产地蓝美1号加工产品及其电子舌PCA分析结果 图A从左至右依次为果脯、果酱和果汁。
Fig.4 Processed products of Lanmei 1 from Sichuan production area and the results of their electronic tongue PCA analysis From left to right in Figure A are preserved fruit, jam, and fruit juice.
| 产品Product | 组织形态Tissue form | 色泽Colour | 气味Aroma | 口感Taste | 总得分Total score |
|---|---|---|---|---|---|
| 果脯Preserved | 23.30±1.68 a | 23.60±0.80 a | 23.20±1.08 a | 23.70±0.78 a | 93.80±1.89 a |
| 果酱Jam | 14.30±1.35 b | 12.90±1.37 b | 18.10±1.87 c | 21.90±1.14 b | 67.20±2.86 c |
| 果汁Juice | 23.30±0.64 a | 23.90±0.83 a | 22.00±1.18 b | 19.20±1.72 c | 88.40±1.56 b |
表4 四川产地蓝美1号加工产品的感官评价结果
Table 4 Sensory evaluation results of processed products from blueberry variety Lanmei 1 in Sichuan production area
| 产品Product | 组织形态Tissue form | 色泽Colour | 气味Aroma | 口感Taste | 总得分Total score |
|---|---|---|---|---|---|
| 果脯Preserved | 23.30±1.68 a | 23.60±0.80 a | 23.20±1.08 a | 23.70±0.78 a | 93.80±1.89 a |
| 果酱Jam | 14.30±1.35 b | 12.90±1.37 b | 18.10±1.87 c | 21.90±1.14 b | 67.20±2.86 c |
| 果汁Juice | 23.30±0.64 a | 23.90±0.83 a | 22.00±1.18 b | 19.20±1.72 c | 88.40±1.56 b |
| [1] | WU Y Q, HAN T Y, YANG H, et al. Known and potential health benefits and mechanisms of blueberry anthocyanins: a review[J]. Food Bioscience, 2023, 55: 103050. |
| [2] | 张晓晓, 黄午阳, 於虹, 等. 不同种植地区蓝莓果中花色苷的分布[J]. 中国食品学报, 2022, 22(10): 314-324. |
| ZHANG X X, HUANG W Y, YU H, et al. The distribution of anthocyanins in blueberry fruits from different growing locations[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22(10): 314-324. | |
| [3] | WEI Z W, YANG H Y, SHI J, et al. Effects of different light wavelengths on fruit quality and gene expression of anthocyanin biosynthesis in blueberry (Vaccinium corymbosm)[J]. Cells, 2023, 12(9): 1225. |
| [4] | ZHOU Y J, LIU Y Q, ZHANG X, et al. Effects of soil properties and microbiome on highbush blueberry (Vaccinium corymbosum) growth[J]. Agronomy, 2022, 12(6): 1263. |
| [5] | LIU Z L, XIE L, ZIELINSKA M, et al. Improvement of drying efficiency and quality attributes of blueberries using innovative far-infrared radiation heating assisted pulsed vacuum drying (FIR-PVD)[J]. Innovative Food Science & Emerging Technologies, 2022, 77: 102948. |
| [6] | MICHALSKA A, ŁYSIAK G. Bioactive compounds of blueberries: post-harvest factors influencing the nutritional value of products[J]. International Journal of Molecular Sciences, 2015, 16(8): 18642-18663. |
| [7] | CABANILLAS-BOJORQUEZ L A, VALDEZ-BARO O, GUTIERREZ-GRIJALVA E P, et al. Antioxidant capacity and evaluation of total phenolic content of optimized blueberry (Vaccinium corymbosum) wine[J]. Acta Scientiarum Technology, 2021, 43: e55134. |
| [8] | SANDRI I G, FONTANA R C, BARFKNECHT D M, et al. Clarification of fruit juices by fungal pectinases[J]. LWT-Food Science and Technology, 2011, 44(10): 2217-2222. |
| [9] | TANG C Q, GU Q Y, LI X W, et al. Hydroxycinnamate decarboxylase in Metschnikowia pulcherrima promotes the formation of vinylphenolic pyranoanthocyanins to enhance the color stability of blueberry wine[J]. LWT, 2024, 207: 116633. |
| [10] | 乔慧茹, 房祥军, 吴伟杰, 等. 蓝莓与铁皮石斛叶复合乳酸菌发酵饮料工艺优化与品质分析[J]. 浙江农业学报, 2025, 37(3): 654-666. |
| QIAO H R, FANG X J, WU W J, et al. Process optimization and quality analysis of composite lactic acid bacteria fermented beverage with blueberries and Dendrobium officinale leaves[J]. Acta Agriculturae Zhejiangensis, 2025, 37(3): 654-666. | |
| [11] | PEREZ C, TAGLIANI C, ARCIA P, et al. Blueberry by-product used as an ingredient in the development of functional cookies[J]. Food Science and Technology International, 2018, 24(4): 301-308. |
| [12] | 张琴, 黄世安, 林欣, 等. 基于UPLC-MS/MS的3个李品种果实初生代谢物分析[J]. 食品科学, 2022, 43(16): 226-234. |
| ZHANG Q, HUANG S A, LIN X, et al. Analysis of primary metabolites in three plum varieties using ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Food Science, 2022, 43(16): 226-234. | |
| [13] | 姚沛琳, 刘梦茹, 杨澳, 等. 基于非靶向代谢组学的蓝莓酵素和沙棘酵素代谢产物特征比较[J]. 食品工业科技, 2022, 43(19): 160-166. |
| YAO P L, LIU M R, YANG A, et al. Comparison of metabolite characteristics of blueberry jiaosu and sea-buckthorn jiaosu based on non-targeted metabolomics approach[J]. Science and Technology of Food Industry, 2022, 43(19): 160-166. | |
| [14] | 王倩, 张长青, 李广平, 等. 基于UPLC-QTOF/MS的蓝莓果实发育代谢组学差异分析[J]. 江苏农业科学, 2020, 48(24): 148-152. |
| WANG Q, ZHANG C Q, LI G P, et al. Metabolomics difference analysis of blueberry fruit development based on UPLC-QTOF/MS[J]. Jiangsu Agricultural Sciences, 2020, 48(24): 148-152. | |
| [15] | 韩延超, 陈慧芝, 牛犇, 等. 振动胁迫对蓝莓花色苷代谢及相关基因表达的影响[J]. 浙江农业学报, 2024, 36(3): 622-633. |
| HAN Y C, CHEN H Z, NIU B, et al. Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry[J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 622-633. | |
| [16] | GHIRARDO A, FOCHI V, LANGE B, et al. Metabolomic adjustments in the orchid mycorrhizal fungus Tulasnella calospora during symbiosis with Serapias vomeracea[J]. New Phytologist, 2020, 228(6): 1939-1952. |
| [17] | CHEN S, LI M H, ZHENG G Y, et al. Metabolite profiling of 14 Wuyi rock tea cultivars using UPLC-QTOF MS and UPLC-QqQ MS combined with chemometrics[J]. Molecules, 2018, 23(2): 104. |
| [18] | 曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007. |
| [19] | ZHANG Y Q, WANG H X, CHEN H J, et al. The crucial evaluation indexes and relative measurement methods of edible value for fresh fruits and vegetables: a review[J]. Future Postharvest and Food, 2024, 1(2): 222-236. |
| [20] | 王晓艳. 物流振动对蓝莓贮藏品质及花色苷代谢的影响[D]. 舟山: 浙江海洋大学, 2022. |
| WANG X Y. Effects of logistics vibration on storage quality and anthocyanin metabolism in blueberries[D]. Zhoushan: Zhejiang Ocean University, 2022. | |
| [21] | 刘慧, 刘杰超, 吕真真, 等. 加工工艺对蓝莓汁和蓝莓酒中花色苷及类黄酮的影响[J]. 中国酿造, 2018, 37(8): 158-162. |
| LIU H, LIU J C, LV Z Z, et al. Effect of processing technology on the contents of anthocyanins and flavonoids in blueberry juice and wine[J]. China Brewing, 2018, 37(8): 158-162. | |
| [22] | FRACASSETTI D, DEL BO’ C, SIMONETTI P, et al. Effect of time and storage temperature on anthocyanin decay and antioxidant activity in wild blueberry (Vaccinium angustifolium) powder[J]. Journal of Agricultural and Food Chemistry, 2013, 61(12): 2999-3005. |
| [23] | 刘艺, 李冰, 鲁宝君, 等. 多指标综合评价不同产区蓝莓的品质[J]. 北方园艺, 2020(8): 15-23. |
| LIU Y, LI B, LU B J, et al. Comprehensive evaluation of blueberry quality in different producing areas by multiple indicators[J]. Northern Horticulture, 2020(8): 15-23. | |
| [24] | 刘军波, 邹礼根, 邱静, 等. 蓝莓果干脱水加工工艺[J]. 浙江农业科学, 2021, 62(9): 1867-1869. |
| LIU J B, ZOU L G, QIU J, et al. Study on dehydration process of blueberry[J]. Journal of Zhejiang Agricultural Sciences, 2021, 62(9): 1867-1869. | |
| [25] | 王少娟, 党娅, 赵桦. 蓝莓果酱加工工艺技术研究[J]. 黑龙江农业科学, 2016(7): 102-107. |
| WANG S J, DANG Y, ZHAO H. Researchon processing technology of blueberry jam[J]. Heilongjiang Agricultural Sciences, 2016(7): 102-107. | |
| [26] | 唐卿雁, 廖小琳, 杨丽丽, 等. 蓝莓果汁饮品配方优化及品质分析[J]. 食品研究与开发, 2022, 43(5): 115-122. |
| TANG Q Y, LIAO X L, YANG L L, et al. Formula optimization and quality analysis of blueberry juice beverage[J]. Food Research and Development, 2022, 43(5): 115-122. | |
| [27] | TASA T A, STARAST M, VOOL E, et al. Influence of soil type on half-highbush blueberry productivity[J]. Agricultural and Food Science, 2012, 21(4): 409-420. |
| [28] | 吕芳楠, 温靖, 徐玉娟, 等. 蓝莓果实的营养价值和药理作用及其加工利用[J]. 安徽农业科学, 2016, 44(18): 85-87. |
| LYU F N, WEN J, XU Y J, et al. Nutritional value, pharmacological action and processing and utilization of blueberry fruits[J]. Journal of Anhui Agricultural Sciences, 2016, 44(18): 85-87. | |
| [29] | 李斌, 鲍义文, 李佳欣, 等. 蓝莓加工副产物资源综合利用及新业态发展趋势[J]. 食品科学技术学报, 2024, 42(4): 1-10. |
| LI B, BAO Y W, LI J X, et al. Comprehensive utilization of blueberry processing by-product resources and development trend of new business forms[J]. Journal of Food Science and Technology, 2024, 42(4): 1-10. | |
| [30] | ADUNOLA P M, FERRÃO L F V, AZEVEDO C F, et al. The effect of environmental variables on the genotyping-by-environment interaction in blueberry[J]. Euphytica, 2024, 220(7): 113. |
| [31] | 欧晓华, 王储炎, 李珂昕, 等. 安徽省主栽蓝莓品种果实的综合品质研究[J]. 中国南方果树, 2019, 48(2): 125-129. |
| OU X H, WANG C Y, LI K X, et al. Integrated quality of main blueberry cultivars in Anhui Province[J]. South China Fruits, 2019, 48(2): 125-129. | |
| [32] | 韩斯, 孟宪军, 汪艳群, 等. 不同品种蓝莓品质特性及聚类分析[J]. 食品科学, 2015, 36(6): 140-144. |
| HAN S, MENG X J, WANG Y Q, et al. Quality properties and cluster analysis of different blueberry cultivars[J]. Food Science, 2015, 36(6): 140-144. | |
| [33] | 熊喜红, 徐晓云, 张强, 等. 湖北和云南地区蓝莓果实加工特性研究[J]. 北方园艺, 2020(2): 23-31. |
| XIONG X H, XU X Y, ZHANG Q, et al. Study on processing quality characteristics of blueberry fruit from Hubei and Yunnan Provinces[J]. Northern Horticulture, 2020(2): 23-31. | |
| [34] | DEL BO’ C, RISO P, BRAMBILLA A, et al. Blanching improves anthocyanin absorption from highbush blueberry (Vaccinium corymbosum L.) purée in healthy human volunteers: a pilot study[J]. Journal of Agricultural and Food Chemistry, 2012, 60(36): 9298-9304. |
| [35] | SANNI-OLAYIWOLA H O, KOLAWOLE F L, ONIFADE F O, et al. Phytochemical composition, antioxidant activities and anti-obesity potential of selected vegetables as affected by drying methods[J]. Future Postharvest and Food, 2024, 1(3): 360-375. |
| [36] | SYAMALADEVI R M, ANDREWS P K, DAVIES N M, et al. Storage effects on anthocyanins, phenolics and antioxidant activity of thermally processed conventional and organic blueberries[J]. Journal of the Science of Food and Agriculture, 2012, 92(4): 916-924. |
| [37] | 张雨, 韩延超, 刘瑞玲, 等. FIRD-HPD方式对蓝莓干燥特性及品质的影响[J]. 中国食品学报, 2024, 24(6): 237-247. |
| ZHANG Y, HAN Y C, LIU R L, et al. Effect of FIRD-HPD method on drying characteristics and quality of blueberry[J]. Journal of Chinese Institute of Food Science and Technology, 2024, 24(6): 237-247. | |
| [38] | ZHANG L L, WANG W B, YUE X Y, et al. Gallic acid as a copigment enhance anthocyanin stabilities and color characteristics in blueberry juice[J]. Journal of Food Science and Technology, 2020, 57(4): 1405-1414. |
| [1] | 李文珏, 杜辰飞, 魏春艳, 蔡丹英, 王月志, 施泽彬, 戴美松, 徐凯. 低温贮藏下梨果实代谢及其调控技术研究进展[J]. 浙江农业学报, 2026, 38(4): 824-836. |
| [2] | 乔慧茹, 房祥军, 吴伟杰, 刘瑞玲, 陈杭君, 邓尚贵, 沙浩, 郜海燕. 蓝莓与铁皮石斛叶复合乳酸菌发酵饮料工艺优化与品质分析[J]. 浙江农业学报, 2025, 37(3): 654-666. |
| [3] | 韩延超, 陈慧芝, 牛犇, 张小栓, 韩树人, 王晓艳, 王冠楠, 刘瑞玲, 郜海燕. 振动胁迫对蓝莓花色苷代谢及相关基因表达的影响[J]. 浙江农业学报, 2024, 36(3): 622-633. |
| [4] | 李璐, 韩强, 吴伟杰, 房祥军, 郜海燕. 蓝莓苹果重组蜜饯干燥工艺优化及对品质的影响[J]. 浙江农业学报, 2019, 31(5): 816-822. |
| [5] | 康海婷, 凌丹燕, 吴志娟, 路梅, 宗宇, 郭卫东. 蓝莓枝干溃疡病病原鉴定及品种抗病性研究[J]. 浙江农业学报, 2019, 31(3): 436-443. |
| [6] | 任艳玲1, 田虹2, 王涛2,*, 蔡秋2, 陈霄2, 王兴宁2, 于晓飞3, 罗阿东2, 柯绍英2, 欧后丁3, 黄碟2. 出口蓝莓基地病虫害调查初报[J]. 浙江农业学报, 2016, 28(6): 1025-. |
| [7] | 薛璐1,刘小路1,鲁晓翔1,张鹏2,陈绍慧2,李江阔2. 近红外漫反射无损检测蓝莓硬度的研究[J]. 浙江农业学报, 2015, 27(9): 1646-. |
| [8] | 杨乐,侯智霞*,杨俊枫,宫中志,王冲,史文君. UV\|C对蓝莓酚类物质及其相关酶活性的影响[J]. 浙江农业学报, 2015, 27(6): 955-. |
| [9] | 肇莹1,杨涛1,孙进杰2,杨镇1,王红1,肖军1,*. 一株蓝莓菌根真菌的鉴定及对蓝莓生长的影响 [J]. 浙江农业学报, 2015, 27(3): 400-. |
| [10] | 曹雪丹1,方修贵1,周伟东2,戚行江3,赵凯1. 澄清工艺对蓝莓果汁品质的影响[J]. 浙江农业学报, 2014, 26(4): 1042-. |
| [11] | 陈华江;陈伟平;李建华;周伟东;曹雪丹. 浙江省适栽蓝莓品种筛选试验初报[J]. , 2013, 25(4): 0-781. |
| 阅读次数 | ||||||
|
全文 |
|
|||||
|
摘要 |
|
|||||