›› 2011, Vol. 23 ›› Issue (2): 0-272.

• 动物科学 •    

绍兴鸭线粒体基因组全序列测定与分析

涂剑锋,司方方,邢秀梅,徐佳萍,杨福合*   

  1. 中国农业科学院 特产研究所,吉林 吉林 132109
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25

Complete sequence determination and analysis of mitochondrial genome of Shaoxing duck

TU Jian-feng;SI Fang-fang;XING Xiu-mei;XU Jia-ping;YANG Fu-he*   

  1. Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-25 Published:2011-03-25

摘要: 参照近源物种线粒体基因组序列设计15对引物,通过PCR扩增、测序、拼接,获得绍兴鸭(Anas platyrhychos)线粒体基因组全序列并进行序列分析。绍兴鸭线粒体基因组全长16 604 bp,碱基组成为29.20%A,22.19%T,15.78%G,32.83%C,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码控制区(D-loop),基因组成及排列顺序与其他鸟类相似,表明鸟类线粒体DNA进化上有较高的保守性。

关键词: 绍兴鸭, 线粒体基因组, 序列分析

Abstract: Using PCR amplification, sequencing and assembling, the complete mitochondrial genome of Shaoxing duck (Anas platyrhychos) was obtained. The full sequence was acquired from 15 primers designed according to close species mitochondrial genome. The entire mitochondrial genome of Shaoxing duck was 16 604 bp in length, which contained 37 genes (13 protein coding genes, 2 rRNA, 22 tRNA) and a non-coding control region (D-loop). The composition of the nucleotides A, T, G, C was 29.20%, 22.19%, 15.78%, 32.83%, respectively. The gene composition and sequence arrangement was similar to other birds, indicating that mitochondrial DNA of bird was more conservative in evolution.

Key words: Shaoxing duck, mitochondrial genome, sequence analysis