Prevalence and genotyping of Giardia duodenalis in pre-weaned dairy calves in Sichuan Province

doi:10.3969/j.issn.1004-1524.2018.07.03

›› 2018, Vol. 30 ›› Issue (7): 1116-1121.DOI: 10.3969/j.issn.1004-1524.2018.07.03

• Animal Science • Previous Articles Next Articles

Prevalence and genotyping of Giardia duodenalis in pre-weaned dairy calves in Sichuan Province

DAN Jiaming¹, OU Hongping², YAN Guangwen³, TIAN Yinan¹, WEI Bin¹, TU Rui¹, XIAO Qicheng¹, TANG Li¹, YANG Tingyu¹, PENG Guangneng¹, WANG Yonggang⁴, ZHONG Zhijun^{1, *}

1.Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine,Sichuan Agricultural University, Chengdu 611130, China;
2. Chengdu Agricultural College,Chengdu 611130, China;
3. College of Animal Science and Technology, Xichang College, Xichang 615000, China;
4. Veterinary Station of Beichuan Qiang Autonomous County, Mianyang 622750, China

Received:2017-09-25 Online:2018-07-20 Published:2018-08-02

Abstract

Abstract: To investigate the prevalence and molecular characteristics of Giardia duodenalis (G. duodenalis) in pre-weaned dairy calves in Sichuan Province, a total of 278 pre-weaned dairy calves' fecal samples were randomly collected in Sichuan. Samples were examined by the Sheather's sucrose flotation technique, and DNA was extracted. These samples were detected for Giardia by nest PCR amplification of β-giardin (bg), tpi and gdh genes. The results showed that 9.35% (26/278) was positive for G. duodenalis infection. One assemblage was detected, which was assemblage E. Multilocus genotyping showed that 7 subtypes of assemblage including 3, 2 and 2 new subtypes (bg: E13, E14, E16; tpi: E21, E24; gdh: E18, E19) were observed based on bg, tpi and gdh genes, respectively. The results indicated that assemblage E had the ascendant position in pre-weaned dairy calves in Sichuan Province and which showed its high genetic diversity.

Key words: Giardia duodenalis, pre-weaned dairy calves, assemble E

CLC Number:

S852.7

DAN Jiaming, OU Hongping, YAN Guangwen, TIAN Yinan, WEI Bin, TU Rui, XIAO Qicheng, TANG Li, YANG Tingyu, PENG Guangneng, WANG Yonggang, ZHONG Zhijun. Prevalence and genotyping of Giardia duodenalis in pre-weaned dairy calves in Sichuan Province[J]. , 2018, 30(7): 1116-1121.

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Prevalence and genotyping of Giardia duodenalis in pre-weaned dairy calves in Sichuan Province

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