Abstract: In this study, in vitro culture techniques were used to study the effects of different concentrations of 7S (β-conglycinin) on IPEC-J2 (intestinal epithelial cells in piglets). The experiment was divided into six groups, A (control), B (1 mg·mL^-1 7S), C (5 mg·mL^-1 7S), D (10 mg·mL^-1 7S), E (5 mg·mL^-1 7S+1μmol·L^-1 SP600125), F (5 mg·mL^-1 7S+1 μmol·L^-1 SB202190). After 24 hours, the viability of the cells was measured by CCK-8 assay. The contents of NO, 5-HT, IL-6 and IL-10 were detected by ELISA, the expression levels of p-JNK, p-p38 and Bcl-2 proteins were detected by western blot, the relative expression of Bad, Bax, Bcl-2 and Caspase-3 mRNA was detected by PCR. The results showed that the viability of IPEC-J2 cells was decreased in groups C and D (P<0.01). The viability of cells in groups E and F was significantly higher than that in group C (P<0.05). 7S promoted the secretion of inflammatory cytokines NO, 5-HT, and IL-6 and decreased the secretion of IL-10, the addition of inhibitors decreased the secretion of cytokines NO, 5-HT, and IL-6, and increased the secretion of IL-10. 7S promoted the expression of p-JNK and p-p38 proteins and decreased the expression of Bcl-2 protein. The addition of inhibitors inhibited the expression of p-JNK and p-p38 proteins and increased the expression of Bcl-2 protein. The ratio of Bcl-2/Bax mRNA decreased with the increase of 7S concentration, and the ratio of Bax/Bcl-x_l increased with the increase of 7S concentration. The relative expression of Caspase-3 mRNA increased with the increase of 7S concentration. On contrary, often adding inhibitors, the ratio of Bcl-2/Bax increased, the ratio of Bax/Bcl-x_l decreased, and the relative expression of Caspase-3 decreased. Our results indicated that 7S caused damage to the intestinal epithelial cells of piglets through the p38/JNK MAPK signaling pathway.

Key words: β-conglycinin;, IPEC-J2, JNK, P38