Isolation, identification and degradation characteristics of a dibutyl phthalate and di-(2-ethylhexyl) phthalate degrading bacterium

doi:10.3969/j.issn.1004-1524.2019.07.15

›› 2019, Vol. 31 ›› Issue (7): 1145-1153.DOI: 10.3969/j.issn.1004-1524.2019.07.15

• Environmental Science • Previous Articles Next Articles

Isolation, identification and degradation characteristics of a dibutyl phthalate and di-(2-ethylhexyl) phthalate degrading bacterium

LIANG Haohua^1,2, TAO Hong^1,2, WANG Yajuan^2,3,*, LI Jiaoling¹

1. College of Resources and Environment, Ningxia University, Yinchuan 750021, China;
2. Key Laboratory (China-Arab) of Resource Evaluation and Environmental Regulation of Arid Region in Ningxia, Yinchuan 750021, China;
3. College of Economics and Management, Ningxia University, Yinchuan 750021, China

Received:2019-01-22 Online:2019-07-25 Published:2019-08-07

Abstract

Abstract: In order to further study the microbial remediation of major PAEs pollutants in soil, a strain, named as AS001, capable of simultaneously degrading dibutyl phthalate (DBP) and Di-(2-ethylhexyl) phthalate (DEHP) was isolated from facility vegetable soil by culture enrichment method. The strain could utilize DBP and DEHP as the sole source of carbon and energy. Based on the morphology, physiological characteristic and 16S rDNA sequence analysis, AS001 was identified as Arthrobacter sp. and the degradation characteristics of phthalate acid esters were investigated under different rotating speeds, pH, initial concentrations, inoculation amount and temperature. The result showed that the optimal degradation condition of strain AS001 was: the rotation speed 175 r·min^-1, pH 7, initial concentration 100 mg·L^-1, inoculum size (V/V) 4%, temperature 35 ℃, and the degradation rate of DBP was higher than that of DEHP under different conditions. This study provided a certain theoretical basis for the environmental conditions of PAEs pollution remediation in soil in this area.

Key words: dibutyl phthalate, di-(2-ethylhexyl) phthalate, Arthrobacter sp., degradation characteristic

CLC Number:

LIANG Haohua, TAO Hong, WANG Yajuan, LI Jiaoling. Isolation, identification and degradation characteristics of a dibutyl phthalate and di-(2-ethylhexyl) phthalate degrading bacterium[J]. , 2019, 31(7): 1145-1153.

References

[1] WANG J, CHEN G C, CHRISTIE P, et al.Occurrence and risk assessment of phthalate esters (PAEs) in vegetables and soils of suburban plastic film greenhouses[J]. Science of the Total Environment, 2015, 523: 129-137.
[2] 杨志豪, 何明靖, 杨婷, 等. 邻苯二甲酸酯在重庆市城市土壤中的污染分布特征及来源分析[J]. 环境科学, 2018, 39(7): 3358-3364.
YANG Z H, HE M J, YANG T, et al.Occurrence and distribution of phthalate esters in urban soils of Chongqing City[J]. Environmental Science, 2018, 39(7): 3358-3364.(in Chinese with English abstract)
[3] 杨婷, 何明靖, 杨志豪, 等. 邻苯二甲酸酯在三峡库区消落带非淹水期土壤中污染特征及健康风险[J]. 环境科学, 2017, 38(10): 4187-4193.
YANG T, HE M J, YANG Z H, et al.Occurrence, distribution and health risk of the phthalate esters in riparian soil in the fluctuating zone of the Three Gorges Reservoir[J]. Environmental Science, 2017, 38(10): 4187-4193.(in Chinese with English abstract)
[4] LI C, CHEN J Y, WANG J H, et al.Phthalate esters in soil, plastic film, and vegetable from greenhouse vegetable production bases in Beijing, China: concentrations, sources, and risk assessment[J]. The Science of the Total Environment, 2016, 568: 1037-1043.
[5] 徐雪, 王利军, 卢新卫. 咸阳市郊菜地土壤中邻苯二甲酸酯(PAEs)污染研究[J]. 农业环境科学学报, 2014, 33(10): 1912-1919.
XU X, WANG L J, LU X W.Pollution of phthalic acid esters (PAEs) in vegetable soils in Xianyang suburbs, northwest China[J]. Journal of Agro-Environment Science, 2014, 33(10): 1912-1919.(in Chinese with English abstract)
[6] 吴山, 李彬, 梁金明, 等. 汕头市蔬菜产区土壤-蔬菜中邻苯二甲酸酯(PAEs)污染分布特征研究[J]. 农业环境科学学报, 2015, 34(10): 1889-1896.
WU S, LI B, LIANG J M, et al.Distribution characteristics of phthalic acid esters in soils and vegetables in vegetable producing areas of Shantou city, China[J]. Journal of Agro-Environment Science, 2015, 34(10): 1889-1896.(in Chinese with English abstract)
[7] 王冬莹, 吴文成, 李霞, 等. 一株能同时降解邻苯二甲酸二丁酯(DBP)和氰戊菊酯真菌的分离、鉴定及降解特性的初步研究[J]. 环境科学学报, 2015, 35(11): 3493-3499.
WANG D Y, WU W C, LI X, et al.Isolation, identification and degradation characteristics of a dibutyl phthalate and fenvalerate degrading fungal strain[J]. Acta Scientiae Circumstantiae, 2015, 35(11): 3493-3499.(in Chinese with English abstract)
[8] 李方方, 宋建, 吕律, 等. DBP高效降解菌群的富集及其降解特性研究[J]. 环境科学学报, 2017, 37(7):2579-2585.
LI F F, SONG J, LYU L, et al.Enrichment of a highly efficient DBP-degrading bacterium consortium and its degradation characters[J]. Acta Scientiae Circumstantiae, 2017, 37(7):2579-2585. (in Chinese with English abstract)
[9] 高俊贤, 刘琦, 连梓竹, 等. 一株DBP高效降解菌的分离、鉴定与降解性能[J]. 环境工程学报, 2016, 10(3): 1521-1526.
GAO J X, LIU Q, LIAN Z Z, et al.Isolation and identification of highly efficient DBP-degrading strain and its degradation study[J]. Chinese Journal of Environmental Engineering, 2016, 10(3): 1521-1526.(in Chinese with English abstract)
[10] 金雷. 1株DBP高效降解菌H-2的分离鉴定及其降解特性研究[J]. 食品科学, 2014, 35(15):202-206.
JIN L.Isolation and characterization of a DBP-degrading strain H-2 and its degrading characteristics[J]. Food Science, 2014, 35(15):202-206. (in Chinese with English abstract)
[11] 李建龙, 邵向丽, 刘书亮, 等. 邻苯二甲酸二丁酯降解菌株的筛选鉴定及其降解特性研究[J]. 现代食品科技, 2014, 30(10): 108-114.
LI J L, SHAO X L, LIU S L, et al.Isolation and identification of dibutyl phthalate-degrading strain and its degradation characteristics[J]. Modern Food Science and Technology, 2014, 30(10): 108-114.(in Chinese with English abstract)
[12] JIN D C, BAI Z H, CHANG D D, et al. Biodegradation of di-n-butyl phthalate by an isolated Gordonia sp. strain QH-11: genetic identification and degradation kinetics[J]. Journal of Hazardous Materials, 2012, 221/222: 80-85.
[13] 陈湖星, 杨雪, 张凯, 等. 1株高效DBP降解菌的分离与特性研究[J]. 环境科学, 2013, 34(7):2882-2888.
CHEN H X, YANG X, ZHANG K, et al.Isolation and characterization of a highly efficient BBP-degrading bacterium[J]. Environmental Science, 2013, 34(7):2882-2888. (in Chinese with English Abstract)
[14] 陈学斌. DEHP降解菌的分离及其与玉米联合修复受污染土壤[D]. 广州: 暨南大学, 2017.
CHEN X B.Isolation of di(2-ethylhexyl) phthalate degrading bacteria and its combined remediation with maize for contaminated soil[D]. Guangzhou: Jinan University, 2017.(in Chinese with English abstract)
[15] 姚雪松. 邻苯二甲酸二(2-乙基己基)酯降解菌的分离鉴定及降解特性研究[D]. 牡丹江: 牡丹江师范学院, 2016.
YAO X S.Isolation, identification of a DEHP-degrading bacterium and its biodegradation characteristics[D]. Mudanjiang: Mudanjiang Normal College, 2016.(in Chinese with English abstract)
[16] 高静静, 陈丽玮, 王宜青, 等. 一株邻苯二甲酸二(2-乙基己基)酯(DEHP)高效降解菌的筛选及其降解特性[J]. 环境化学, 2016, 35(11): 2362-2369.
GAO J J, CHEN L W, WANG Y Q, et al.Isolation, identification of a DEHP-degrading bacterium and its high effective biodegradation characteristics[J]. Environmental Chemistry, 2016, 35(11): 2362-2369.(in Chinese with English abstract)
[17] 严佳丽, 陈湖星, 杨杨, 等. 一株高效DEHP降解菌的分离、鉴定及其降解特性[J]. 微生物学通报, 2014, 41(8): 1532-1540.
YAN J L, CHEN H X, YANG Y, et al.Isolation and characterization of a highly efficient DEHP-degrading bacterium[J]. Microbiology China, 2014, 41(8): 1532-1540.(in Chinese with English abstract)
[18] 张可, 陈强, 陈伟, 等. 邻苯二甲酸二甲酯(DMP)降解菌的分离鉴定及降解特性[J]. 安全与环境学报, 2017, 17(2): 645-649.
ZHANG K, CHEN Q, CHEN W, et al.On the isolation, identification and characterization of DMP-degrading strain[J]. Journal of Safety and Environment, 2017, 17(2): 645-649.(in Chinese with English abstract)
[19] 金德才, 吴学玲, 梁任星, 等. 一株DMP降解菌的分离鉴定及其降解特性[J]. 微生物学通报, 2009, 36(9): 1318-1323.
JIN D C, WU X L, LIANG R X, et al.Isolation, identification and degradation characteristics of a DMP-degrading strain[J]. Microbiology, 2009, 36(9): 1318-1323.(in Chinese with English abstract)
[20] 褚娇艳, 刘腾飞, 张汉虞, 等. 一株邻苯二甲酸二丁酯降解菌的筛选及其降解特性[J]. 微生物学通报, 2017, 44(7): 1555-1562.
CHU J Y, LIU T F, ZHANG H Y, et al.Screening and characterization of a dibutyl phthalate-degrading bacterium[J]. Microbiology China, 2017, 44(7): 1555-1562.(in Chinese with English abstract)
[21] 周长健. 高效DEHP降解菌的筛选鉴定及其土壤模拟修复初探[D]. 哈尔滨: 东北农业大学, 2016.
ZHOU C J.Isolation of an efficient di-(2-ethylhexyl) phthalate degrading bacteria and preliminary study of the soil remediation[D]. Harbin: Northeast Agricultural University, 2016.(in Chinese with English abstract)
[22] 饶潇潇, 王建超, 周震峰. 花生对土壤中邻苯二甲酸酯的吸收累积特征[J]. 环境科学学报, 2017, 37(4): 1531-1538.
RAO X X, WANG J C, ZHOU Z F.Characteristics of uptake and accumulation of phthalic acid esters in soil by peanut (Arachis hypogaea)[J]. Acta Scientiae Circumstantiae, 2017, 37(4): 1531-1538.(in Chinese with English abstract)
[23] 王冬莹. 农田土壤中典型复合有机污染物的微生物降解研究[D]. 北京: 中国环境科学研究院, 2014.
WANG D Y.Biodegradation of typical organic pollutants in farmland soil[D]. Beijing: Chinese Research Academy of Environmental Sciences, 2014. (in Chinese with English abstract)
[24] 朱旭芬. 现代微生物学实验技术[M]. 杭州: 浙江大学出版社, 2011.
[25] 李莎莎, 曹煜成, 李卓佳, 等. 碳氮营养和培养条件对芽孢杆菌(Bacillus sp.)A4生长的影响[J]. 渔业科学进展, 2017, 38(6): 119-126.
LI S S, CAO Y C, LI Z J, et al.The effects of different carbon and nitrogen nutrients and culture conditions on the growth of Bacillus sp. strain A4[J]. Progress in Fishery Sciences, 2017, 38(6): 119-126.(in Chinese with English abstract)
[26] 雷萍, 吴亚召, 张文隽, 等. 不同搅拌转速和通气量对桑黄深层发酵培养的影响[J]. 食用菌学报, 2014, 21(2): 57-62.
LEI P, WU Y Z, ZHANG W J, et al.Effects of impellor speed and aeration rate on mycelial biomass and extracellular polysaccharide production, reducing sugar consumption and dissolved oxygen levels in fermentor-grown cultures of Phellinus baumii[J]. Acta Edulis Fungi, 2014, 21(2): 57-62.(in Chinese with English abstract)
[27] 韩蕊, 王冬莹, 芮洋, 等. 一株降解邻苯二甲酸酯真菌的筛选及其降解特性研究[J]. 环境科学学报, 2013, 33(11): 2941-2946.
HAN R, WANG D Y, RUI Y, et al.Biodegradation of PAEs by a newly isolated fungus Eupenicillum javanicun F9[J]. Acta Scientiae Circumstantiae, 2013, 33(11): 2941-2946.(in Chinese with English abstract)
[28] 李玉双, 陈琳, 郭倩, 等. 沈阳市新民设施农业土壤中邻苯二甲酸酯的污染特征[J]. 农业环境科学学报, 2017, 36(6): 1118-1123.
LI Y S, CHEN L, GUO Q, et al.Pollution characteristics of phthalate esters in greenhouse agricultural soil in Xinmin, Shenyang City[J]. Journal of Agro-Environment Science, 2017, 36(6): 1118-1123.(in Chinese with English abstract)
[29] 郑顺安, 薛颖昊, 李晓华, 等. 山东寿光设施菜地土壤-农产品邻苯二甲酸酯(PAEs)污染特征调查[J]. 农业环境科学学报, 2016, 35(3): 492-499.
ZHENG S A, XUE Y H, LI X H, et al.Phthalate acid esters (PAEs) pollution in soils and agricultural products of vegetable greenhouses in Shouguang City, Shandong Province[J]. Journal of Agro-Environment Science, 2016, 35(3): 492-499.(in Chinese with English abstract)
[30] 郭静波, 陈微, 姜丽杰, 等. 邻苯二甲酸二丁酯生物降解研究进展[J]. 应用与环境生物学报, 2014, 20(6): 1104-1110.
GUO J B, CHEN W, JIANG L J, et al.Research progresses in dibutyl phthalate biodegradation[J]. Chinese Journal of Applied and Environmental Biology, 2014, 20(6): 1104-1110.(in Chinese with English abstract)

Isolation, identification and degradation characteristics of a dibutyl phthalate and di-(2-ethylhexyl) phthalate degrading bacterium

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	XU Shuangyan, ZHANG Tao, ZHANG Cheng, LIN Hui, SHUI Xianlei, ZHENG Huabao. Isolation and identification of an erythromycin degradation bacterium strain and its biodegradation characteristics [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 131-141.
[2]	GAO Wenjing, XIAO Lijiao, WANG Shunmin, HAN Qiuxia. Screening, identification and characterization of two halophilic, diesel-degrading bacteria [J]. , 2020, 32(7): 1241-1252.