Observations on infection of Nilaparvata lugens (Stål) by Metarhizium flavoviride

doi:10.3969/j.issn.1004-1524.2019.08.16

›› 2019, Vol. 31 ›› Issue (8): 1345-1352.DOI: 10.3969/j.issn.1004-1524.2019.08.16

• Plant Protection • Previous Articles Next Articles

Observations on infection of Nilaparvata lugens (Stål) by Metarhizium flavoviride

ZHANG Juefeng^1,2, CHEN Jianming², LI Fang², ZHONG Haiying², WU Hong^1,*

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-04-04 Online:2019-08-25 Published:2019-08-30

Abstract

Abstract: In the present study, the process of Metarhizium flavoviride conidia infecting Nilaparvata lugens(Stål) and its eggs was observed by using a scanning electron microscope (SEM) and semithin section, and the effects of M. flavoviride on hatching rate and adult and nymph development duration were also detected. The results showed that M. flavoviride invaded the internode membranes on cuticle preferentially, form appressoriums, the appressorium germinated and formed an invading mycelia to invade the host's blood cavity. Mycelia multiplied rapidly using host's nutrition and accumulated in the abdomen, which caused the death of the host. M. flavoviride sprayed on rice plants could not infect N. lugens eggs produced in the leaf sheath, but could colonize and reproduce on eggs in vitro. The hatching rate of N. lugens infected by M. flavoviride was in the range of 61.10%-72.67% mostly, and the prolongation of treatment time did not decrease the hatching rate of N. lugens. There was no significant difference in duration of nymphs and adults among different treatments. Therefore, M. flavoviride could infect cuticle and eggs of N. lugens, yet the process took a long time and was susceptible to external factors. Thus, rational strategy of using M. flavoviride as a control agent against N. lugens should take external factors well into account.

Key words: Metarhizium flavoviride, Nilaparvata lugens (Stål), scanning electron microscope, semithin section, developmental duration

CLC Number:

ZHANG Juefeng, CHEN Jianming, LI Fang, ZHONG Haiying, WU Hong. Observations on infection of Nilaparvata lugens (Stål) by Metarhizium flavoviride[J]. , 2019, 31(8): 1345-1352.

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Observations on infection of Nilaparvata lugens (Stål) by Metarhizium flavoviride

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