Study on chromosome technique of Calendula officinalis and its karyotype

doi:10.3969/j.issn.1004-1524.2020.01.11

›› 2020, Vol. 32 ›› Issue (1): 86-92.DOI: 10.3969/j.issn.1004-1524.2020.01.11

• Horticultural Science • Previous Articles Next Articles

Study on chromosome technique of Calendula officinalis and its karyotype

LONG Haimei^1,2, ZHANG Nanqing¹, LI Zongyan^1,2,*, WANG Qin¹, FU Chao¹

1. Southwest Engineering Research Center of Landscape Architecture, National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China;
2. Functional Flower Center, Southwest Forestry University, Kunming 650224, China

Received:2019-05-27 Online:2020-01-25 Published:2020-03-11

Abstract

Abstract: In order to explore the optimal chromosome technique of Calendula officinalis, its root tips were used to investigate the effects of sampling time, root length, pretreatment methods and different reagent. The results indicated that Calendula officinalis could maintain vigorous mitotic-activity with more cells at mitotic metaphase if the roots were sampled from 8:30 am to 9:30 am when they had 1.0-1.5 cm length. It was better for Calendula officinalis to be treated with saturated solution of p-dichlorobenzene for 6 h at 6 ℃. It showed that the autosomes number of Calendula officinalis was 28 consisting of median region and submedian region types of chromosomes. Moreover, the percentage of all chromosome' relative length varied from 5.93% to 8.46%. The mean indice of relative length ranged from 0.83 to 1.18. Maximum value of arm ratio was 2.34. Besides standard chromosomes, there were bar-like or point-like B-chromosomes in its somatic cells and its number varied from 11 to 18. The analysis showed that the karyotype formula was 2n=18m+10sm and its karyotype belonged to 2B based on Stebbins classification system.

Key words: Calendula officinalis, root tip, chromosome technique, karyotype analysis

CLC Number:

S667.3

LONG Haimei, ZHANG Nanqing, LI Zongyan, WANG Qin, FU Chao. Study on chromosome technique of Calendula officinalis and its karyotype[J]. , 2020, 32(1): 86-92.

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Study on chromosome technique of Calendula officinalis and its karyotype

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