Transcriptome analysis of fruiting bodies of Lactarius deliciosus at two developmental stages

doi:10.3969/j.issn.1004-1524.2020.02.18

›› 2020, Vol. 32 ›› Issue (2): 337-347.DOI: 10.3969/j.issn.1004-1524.2020.02.18

• Food Science • Previous Articles Next Articles

Transcriptome analysis of fruiting bodies of Lactarius deliciosus at two developmental stages

SONG Zhiqiang¹, DING Xiang², TANG Xian¹, ZHU Miao¹, HOU Yiling^1,*

1. Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education, College of Life Sciences, China West Normal University, Nanchong 637009, China;
2. College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China

Received:2019-05-10 Online:2020-02-25 Published:2020-03-13

Abstract

Abstract: In this study, the fruiting bodies of Lactarius deliciosus were sequenced by de novo sequencing and bioinformatics analysis. The differences of gene expression between young mushroom (LDG-1) and mature mushroom (LDG-2) during the growth and development of fruiting bodies of Lactarius deliciosus were explored, and the main genes and metabolic pathways related to the growth and development of Lactarius deliciosus were also studied. The sequencing results showed that a total of 7.44G (LDG-1) and 7.21G (LDG-2) clean reads were obtained. The results of KOG functional annotation showed that Lactarius deliciosus had comprehensive and complex gene functional categories at LDG-1 and LDG-2. The results of GO enrichment indicated that there were some differences in the bio-metabolic functions of Lactarius deliciosus at different growth stages (LDG-1 and LDG-2). The analysis of gene expression level and cluster showed that the main high-expression genes were ATPase, multifunctional chaperone family genes, etc., which played an important role in energy metabolism and cell cycle regulation. Analysis of differentially expressed genes showed that compared with LDG-1, there were 16 789 differentially expressed genes in LDG-2, in which 7 635 genes were up-regulated and 9 154 genes were down-regulated. KEGG pathway enrichment of differential expression gene results showed that oxidative phosphorylation was the key pathway of energy metabolism during the growth and development of Lactarius deliciosus. Further analysis showed that MAPK signaling pathway was the key signaling pathway affecting the development of fruiting body of Lactarius deliciosus, which plays an important role in promoting sexual reproduction and regulating polar growth of mycelium of fruiting body of Lactarius deliciosus.

Key words: Lactarius deliciosus, de novo sequencing, differentially expressed genes, oxidative phosphorylation, MAPK signaling pathway

CLC Number:

S646

SONG Zhiqiang, DING Xiang, TANG Xian, ZHU Miao, HOU Yiling. Transcriptome analysis of fruiting bodies of Lactarius deliciosus at two developmental stages[J]. , 2020, 32(2): 337-347.

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Transcriptome analysis of fruiting bodies of Lactarius deliciosus at two developmental stages

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