Study on mRNA expression pattern of IGF-1R gene in chicken skeletal muscles during early development

doi:10.3969/j.issn.1004-1524.2020.07.04

›› 2020, Vol. 32 ›› Issue (7): 1160-1165.DOI: 10.3969/j.issn.1004-1524.2020.07.04

• Animal Science • Previous Articles Next Articles

Study on mRNA expression pattern of IGF-1R gene in chicken skeletal muscles during early development

SHENG Zhongwei, JI Gaige, LIU Yifan, JU Xiaojun, SHAN Yanju, ZOU Jianmin, ZHANG Ming, TU Yunjie, SHU Jingting^*

Jiangsu Institute of Poultry Science,Yangzhou 225125,China

Received:2019-09-30 Online:2020-07-25 Published:2020-07-28

Abstract

Abstract: In the present study, the Huashan and Qingyuan partridge chickens differing in growth rates were used as experimental materials,fluorescent real-time quantitative PCR (RT-PCR) was used to detect the mRNA expression pattern of IGF1R gene in pectoral and leg muscle of chickens on the embryonic days of 9, 12, 16, 21 and 7 days post-hatching. The results showed that pectoral muscle weight significantly decreased in the two breeds at the embryonic days 21, but leg muscle weigh significantly increased as time going. IGF1R mRNA expression tendency of pectoral muscle in Huashan partridge chickens were from the high to the low: the embryonic days 9 was an expression peak, and then the expression level significantly decreased and kept a low level and down-regulated in 7 days post-hatching again. IGF1R mRNA expression of pectoral muscle in Qingyuan partridge chickens were a wavy trend: the expression levels of IGF1R gene on pectorals at 9 and 16 embryo age were higher, others were significant down-regulated. In leg muscle, the expression levels of IGF1R gene were significant down-regulated after 16 embryo age in Huashan partridge chicken; the expression levels of IGF1R gene in Qingyuan partridge chickens were decreased gradually and there were significant differences among groups(P<0.05). The expression level of IGF1R gene in pectoral and leg muscles were significantly different between varieties at different time(P<0.05). The expression level of IGF1R gene were significantly correlated with the weight of pectoral and leg muscles, respectively (P<0.01). The above results were preliminarily revealed the development changes and difference of IGF1R gene expression in pectoral and leg muscles during early development of different breeds, provide basic data for further study on regulation mechanism of chicken muscle development.

Key words: chicken, skeletal muscle, early development, IGF1R, mRNA expression

CLC Number:

S831

SHENG Zhongwei, JI Gaige, LIU Yifan, JU Xiaojun, SHAN Yanju, ZOU Jianmin, ZHANG Ming, TU Yunjie, SHU Jingting. Study on mRNA expression pattern of IGF-1R gene in chicken skeletal muscles during early development[J]. , 2020, 32(7): 1160-1165.

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Study on mRNA expression pattern of IGF-1R gene in chicken skeletal muscles during early development

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