Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25

doi:10.3969/j.issn.1004-1524.2020.08.03

›› 2020, Vol. 32 ›› Issue (8): 1351-1356.DOI: 10.3969/j.issn.1004-1524.2020.08.03

• Animal Science • Previous Articles Next Articles

Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25

WANG Xiaowei¹, FAN Yi², MA Xiaoming¹, MA Lina¹, YUE Caijuan¹, WANG Jin¹, ZHAO Zhengwei¹, MA Qing^1,*

1. Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750000, China;
2. Yinchuan Zhongke Yuanhao Technology Co., Ltd., Yinchuan 750000, China

Received:2020-02-11 Online:2020-08-25 Published:2020-08-28

Abstract

Abstract: In order to explore the relationship between the melanocortin-1-receptor gene (MC1R) and transcription factor 25 gene(TCF25), which affect the coat colors of mammals, and the coat colors of Tan sheep, real-time fluorescent quantitative PCR (qRT-PCR) technique was used to study the mRNA expression of MC1R gene and TCF25 gene in skin tissues of 15 Tan sheep with different coat colors (pure white, brown spot and black spot). The results showed that MC1R gene and TCF25 gene were expressed in three different kinds of coat colors of Tan sheep. Expression quantity of MC1R gene in skin tissues of different coat colors of Tan sheep was black spot>brown spot>pure white. The black spot group was significantly higher than the pure white group (P<0.01), the brown spot group was significantly higher than the brown spot group (P<0.05), and the brown spot group was significantly higher than the pure white group (P<0.05). The expression quantity of TCF25 gene in skin tissues of different coat colors of Tan sheep was pure white>brown spot>black spot, with no significant difference among groups (P>0.05).The results showed the relationship between the expression level of target gene and the coat colors of Tan sheep, and provided references for the color breeding and improvement of Tan sheep in the future.

Key words: Tan sheep, coat color, MC1R gene, TCF25 gene

CLC Number:

S818.9

WANG Xiaowei, FAN Yi, MA Xiaoming, MA Lina, YUE Caijuan, WANG Jin, ZHAO Zhengwei, MA Qing. Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25[J]. , 2020, 32(8): 1351-1356.

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Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25

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