Transcriptome analysis of red bud taro and green stem taro in Yanshan, Jiangxi Province

doi:10.3969/j.issn.1004-1524.2020.09.02

›› 2020, Vol. 32 ›› Issue (9): 1533-1543.DOI: 10.3969/j.issn.1004-1524.2020.09.02

• Crop Science • Previous Articles Next Articles

Transcriptome analysis of red bud taro and green stem taro in Yanshan, Jiangxi Province

YIN Minghua, CAO Qing, CHEN Hong, DENG Siyu, DENG Yanmei

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2020-02-15 Online:2020-09-25 Published:2020-10-10

Abstract

Abstract: In order to explore the molecular differences between Jiangxi Yanshan red bud taro and Jiangxi Yanshan green stem taro, the transcriptome analysis was carried out on the plantlets of Jiangxi Yanshan red bud taro (HYY group) and Jiangxi Yanshan green stem taro (QGY group). The results showed that the Clean reads of HYY group was 41 298 676, and the GC content was 53.09%; the Clean reads of QGY group was 45 551 860, and the GC content was 51.17%. In HYY group and QGY group, the pair value of expression FPKM was between 0-2, and the expression density was between 0-0.7. The number of common genes expressed in HYY group and QGY group was 14 038, the number of genes independently expressed in HYY group was 17 843, the number of genes independently expressed in QGY group was 18 634. The correlation coefficient of the expression of HYY group and QGY group was 0.035, and the correlation between samples was poor. There were 32 555 differential expressed genes (DEG) in HYY group and QGY group. Compared with QGY group, there were 15 887 up-regulated genes and 16 668 down regulated genes in HYY group. Go enrichment analysis showed that differential genes were mainly annotated to polysaccharide metabolic process, gene silencing, pectin catabolic process, carbohydrate metabolic process, auxin-activated signaling pathway, extracellular region, intrinsic component of membrane, integral component of membrane, membrane part, apoplast, hydrolase activity, acting on glycosyl bonds, hydrolase activity, hydrolyzing O-glycosyl compounds, protein dimerization activity, DNA binding, oxidoreductase activity, acting on diphenols and related substances as donors and other functions. KEGG enrichment analysis showed that the differential genes were mainly annotated to plant hormone signal transduction, plant-pathogen interaction, cysteine and methionine metabolism, phenylpropanoid biosynthesis, cortisol synthesis and secretion, peroxisome, MAPK signaling pathway-plant, mismatch repair, starch and sucrose metabolism, sulfur metabolism, pentose and glucuronate interconversions, C-type lectin receptor signaling pathway, glycerolipid metabolism, biosynthesis of unsaturated fatty acids, alpha-linolenic acid metabolism, diterpenoid biosynthesis, flavonoid biosynthesis, fatty acid elongation, photosynthesis, indole alkaloid biosynthesis and other pathways. The results of this study will provide reference for variety identification and molecular breeding of Jiangxi red bud taro and Jiangxi green stem taro.

Key words: Jiangxi Yanshan red bud taro, Jiangxi Yanshan green stem taro, transcriptome analysis

CLC Number:

S532

YIN Minghua, CAO Qing, CHEN Hong, DENG Siyu, DENG Yanmei. Transcriptome analysis of red bud taro and green stem taro in Yanshan, Jiangxi Province[J]. , 2020, 32(9): 1533-1543.

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Transcriptome analysis of red bud taro and green stem taro in Yanshan, Jiangxi Province

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