Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.

doi:10.3969/j.issn.1004-1524.2021.03.14

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 490-496.DOI: 10.3969/j.issn.1004-1524.2021.03.14

• Plant Protection • Previous Articles Next Articles

Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.

ZHU Haixia, MA Yongqiang, XIAN Wenrong^*()

Academy of Agriculture and Forestry Sciences of Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Scientific Observing and Experimental Station of Crop Pest in Xining of Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province, Xining 810016, China

Received:2020-06-08 Online:2021-04-02 Published:2021-03-25
Contact: XIAN Wenrong

Abstract

Abstract:

Avena fatua L. as a common weed in the farmland of Qinghai Province, was selected as the target receptor. The effects of Trichoderma polysporum HZ-31 on various physiological indexes of wild oats were determined after potted inoculation, and the physiological mechanism of the interaction between host and pathogen was analyzed. The results showed that there were various effects of Trichoderma polysporum HZ-31 on the defense enzymes of physiological mechanism of Avena fatua L., PAL and POD activity increased to resist the infection of the strain. The other physiological defense indexes including MDA content, CAT activity, PPO activity, SOD activity, soluble protein content and chlorophyll content were destroyed by the infection of the strain. The effect of the inhibited defense enzyme was greater than that of the promoted defense enzyme. The infection of HZ-31 inactivated or inhibited the main enzymes in Avena fatua L., interrupted the corresponding enzymatic reaction, and caused a wide range of metabolic changes in plants, including changes in cell membrane permeability, interference in photosynthesis, protein synthesis, phenol metabolism and other physiological changes.

Key words: Trichoderma polysporum, Avena fatua L., infection, physiological mechanism

CLC Number:

S451.1

ZHU Haixia, MA Yongqiang, XIAN Wenrong. Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 490-496.

Figures/Tables 6

Fig.1 Growth performance of Avena fatua L. 3, 5 ,7 days after HZ-31 inoculation

Fig.2 Analysis of the changes of MDA content in A. fatua after HZ-31 inoculation

Fig.3 Analysis of the changes of CAT, POD and SOD content in A. fatua after HZ-31 inoculation

Fig.4 Analysis of the changes of PPO and PAL activity in A. fatua after HZ-31 inoculation

Fig.5 Analysis of the changes of soluble protein content in A. fatua after HZ-31 inoculation

Fig.6 Analysis of the changes of chlorophyll content in A. fatua after HZ-31 inoculation

References 20

[1]	李亚玲, 龙书生, 郭军战 , 等. 玉米感染禾谷镰刀菌后PAL、POD活性和同工酶谱的变化[J]. 西北植物学报, 2003,23(11):1926-1930.
	LI Y L, LONG S S, GUO J Z , et al. Changes of activities of PAL and POD and bands of POD isozyme of Susceptible and resistant corn infected with Fusarium graminearum[J]. Acta Botanica Boreali-Occidentalia Sinica, 2003,23(11):1926-1930.(in Chinese with English abstract)
[2]	黄锐 . 杂交稻及其亲本抗瘟性与几种酶活性及丙二醛含量的关系[D]. 雅安: 四川农业大学, 2007.
	HUANG R . The relationship between blast resistance of hybrid rice or their parent and enzyme activities or MDA content[D]. Ya'an: Sichuan Agricultural University, 2007.(in Chinese with English abstract)
[3]	李捷 . 甘肃省枸杞根腐病病原及生理生化抗病机理研究[D]. 兰州: 甘肃农业大学, 2015.
	LI J . The root rot pathogens of Lycium bararum in Gansu Province and physiological biochemical mechanism of resistance[D]. Lanzhou: Gansu Agricultural University, 2015.(in Chinese with English abstract)
[4]	沈登荣, 何超, 徐慧琼 , 等. 端大蓟马取食对豆科植物防御酶活性及丙二醛含量的影响[J]. 安徽农业大学学报, 2017,44(4):716-719.
	SHEN D R, HE C, XU H Q , et al. Effects of Megalurothrips distalis feeding stress on the activities of defense enzymes and MDA content in leguminous plants[J]. Journal of Anhui Agricultural University, 2017,44(4):716-719.(in Chinese with English abstract)
[5]	孟璐璐, 于春蕾, 薛德胜 , 等. BHT对苹果采后灰霉病的防效及防御酶活性和丙二醛含量的影响[J]. 植物保护学报, 2019,46(3):686-692.
	MENG L L, YU C L, XUE D S , et al. Control efficiency of BHT against postharvest apple grey mold and its influence on the activity of defensive enzymes and content of malondialdehyde[J]. Journal of Plant Protection, 2019,46(3):686-692.(in Chinese with English abstract)
[6]	张秋英, 李发东, 刘孟雨 . 冬小麦叶片叶绿素含量及光合速率变化规律的研究[J]. 中国生态农业学报, 2005,13(3):95-98.
	ZHANG Q Y, LI F D, LIU M Y . Changing laws of chlorophyll content and photosynthetic rate in winter wheat leaves[J]. Chinese Journal of Eco-Agriculture, 2005,13(3):95-98.(in Chinese with English abstract)
[7]	KOCAL N, SONNEWALD U, SONNEWALD S . Cell wall-bound invertase limits sucrose export and is involved in symptom development and inhibition of photosynjournal during compatible interaction between tomato and Xanthomonas campestris pv vesicatoria[J]. Plant Physiology, 2008,148(3):1523-1536. DOI URL PMID
[8]	孟军, 陈温福, 徐正进 , 等. 水稻剑叶净光合速率与叶绿素含量的研究初报[J]. 沈阳农业大学学报, 2001,32(4):247-249.
	MENG J, CHEN W F, XU Z J , et al. Study on photosynthetic rate and chlorophyll content[J]. Journal of Shenyang Agricultural University, 2001,32(4):247-249.(in Chinese with English abstract)
[9]	BERGER S, SINHA A K, ROITSCH T . Plant physiology meets phytopathology: plant primary metabolism and plant-pathogen interactions[J]. Journal of Experimental Botany, 2007,58(15/16):4019-4026. DOI URL
[10]	KIM Y M, BOURAS N, KAV N N V, et al. Inhibition of photosynjournal and modification of the wheat leaf proteome by Ptr ToxB: a host-specific toxin from the fungal pathogen Pyrenophora tritici-repentis[J]. Proteomics, 2010,10(16):2911-2926. DOI URL PMID
[11]	穆西玉, 张海艳, 赵延明 . 粗缩病对不同抗性玉米品种叶片生理特性的影响[J]. 植物生理学报, 2014,50(6):801-804.
	MU X Y, ZHANG H Y, ZHAO Y M . Effects of maize rough dwarf virus(MRDV) on physiological characteristics in leaves of different maize(Zea mays) varieties[J]. Plant Physiology Journal, 2014,50(6):801-804.(in Chinese with English abstract)
[12]	李赤, 于莉, 刘付东标 , 等. 富贵竹中可溶性糖、蛋白质含量与细菌性茎腐病的关系[J]. 吉林农业大学学报, 2007,29(6):620-622.
	LI C, YU L, LIU F , et al. Relation between contents of soluble sugar and protein in Dracaena sanderiana virens and bacterial stem rot[J]. Journal of Jilin Agricultural University, 2007,29(6):620-622.(in Chinese with English abstract)
[13]	李伯凌, 霍本君, 朱寿松 , 等. 木薯叶片组织结构及生理生化特征与其抗细菌性枯萎病的关系[J]. 热带生物学报, 2017,8(3):292-300.
	LI B L, HUO B J, ZHU S S , et al. The structure and physiological characteristics of cassava leaves and their relationship with the resistance to bacterial blight[J]. Journal of Tropical Biology, 2017,8(3):292-300.(in Chinese with English abstract)
[14]	BALASUNDRAM N, SUNDRAM K, SAMMAN S . Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses[J]. Food Chemistry, 2006,99(1):191-203. DOI URL
[15]	高必达, 陈捷 . 生理植物病理学[M]. 北京: 科学出版社, 2006.
[16]	SEWALT V, NI W, BLOUNT J W , et al. Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonia-lyase or cinnamate 4-hydroxylase[J]. Plant Physiology, 1997,115(1):41-50. DOI URL PMID
[17]	程洁 . 白菜、甘蓝根肿病相关生理生化指标的分析[D]. 重庆: 西南大学, 2013.
	CHENG J . Study for the related analysis of physiological and biochemical mechanism in Chinese cabbage and cabbage[D]. Chongqing: Southwest University, 2013.(in Chinese with English abstract)
[18]	PERCY M E . Catalase: an old enzyme with a new role?[J]. Canadian Journal of Biochemistry and Cell Biology, 1984,62(10):1006-1014. URL PMID
[19]	MIYAGAWA Y, TAMOI M, SHIGEOKA S . Evaluation of the defense system in chloroplasts to photooxidative stress caused by paraquat using transgenic tobacco plants expressing catalase from Escherichia coli[J]. Plant and Cell Physiology, 2000,41(3):311-320. DOI URL PMID
[20]	郭新梅, 陈耀锋, 李春莲 , 等. 禾谷镰刀菌粗毒素对不同小麦品种幼苗MDA含量和SOD、PAL活性的影响[J]. 西北植物学报, 2007,27(1):68-73.
	GUO X M, CHEN Y F, LI C L , et al. Effects of Fusarium graminearum crude toxin on MDA contents and SOD and PAL activities in the seedlings of different wheat varieties[J]. Acta Botanica Boreali-Occidentalia Sinica, 2007,27(1):68-73.(in Chinese with English abstract)

Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 20

Related Articles 0

Recommended Articles

Metrics

Comments