Abstract:

In the present study, meat and bone meals were used as fermentation substrate, Bacillus natto NT-6 was used as fermentation and function bacteria, and bacterial manure was produced by soil fermentation.With NT-6 quantity as the main index, the fermentation conditions were optimized by single factor tests and response surface design. On the basis, the effect of the produced bacterial manure against pathogen of the tomato stem rot (Rhizoctonia solani Kühn) were explored. It was shown that the optimal fermentation condition were as follows: the ratio of meat bone to bran was 7.8:2.2, the moisture content was 58.19%, the addition amount of rice husks was 0.82 g, the fermentation time was 100 h. Under this condition, the NT-6 quantity in fermentation product was the highest (2.92×10¹⁰ CFU·g^-1). The produced bacterial manure had no significant promotion effect on seed germination, yet increased biomass of tomato. In soils infected by R. solani, the mortality rate of tomato was 55%, yet it decreased to 20% with application of the produced bacteria manure. In general, meat and bone meals could be used to produce bacteria manure via solid fermentation by B. natto NT-6. The produced bacterial manure not only could enhance the growth of tomato, but also could increase resistance to R. solani.

Key words: Bacillus natto, meat and bone meals, antibacterial peptides, microbial fertilizer