Functional study of MoZds1 in the rice blast fungus

doi:10.3969/j.issn.1004-1524.20230506

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (3): 559-568.DOI: 10.3969/j.issn.1004-1524.20230506

• Plant Protection • Previous Articles Next Articles

Functional study of MoZds1 in the rice blast fungus

HUANG Changli¹(), ZHU Xueming²^,^*(), LI Lin², BAO Jiandong², YU Xiaoping¹, LIN Fucheng¹^,²^,^*()

1. College of Life Sciences, China Jiliang University, Hangzhou 310018, China
2. State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-04-21 Online:2024-03-25 Published:2024-04-09

Abstract

Abstract:

Rice blast disease caused by Magnaporthe oryzae has posed a serious threat to rice yield in China. Studying the gene functions of M. oryzae is beneficial for better understanding the pathogenic mechanism of M.oryzae and lays a foundation for the control of rice blast disease. In this study, a yeast ZDS1 homolog gene MoZDS1 was screened through genetic methods in M. oryzae. The gene deletion mutant ΔMozds1 was successfully obtained by gene knockout method, and it was found that the deletion of MoZDS1 seriously affected the vegetative growth. In order to explore the functions of MoZds1, this study analyzed the roles of Mozds1 in maintaining vegetative growth, conidiation, response to cell wall stress and autophagy. The results showed that MoZds1 positively regulated the mycelium growth and negatively regulated the conidia production. In addition, MoZds1 was also involved in the cell wall integrity pathway and regulated the glycogen degradation in conidia, however, the deletion of MoZds1 did not show a significant effect on the autophagy pathway. Overall, this analysis of the function of MoZds1 systematically in M. oryzae, may provide a theoretical basis for further in-depth research on the function of MoZds1.

Key words: Magnaporthe oryzae, aerogenic mycelium, cell wall integrity, autophagy

CLC Number:

Q786

HUANG Changli, ZHU Xueming, LI Lin, BAO Jiandong, YU Xiaoping, LIN Fucheng. Functional study of MoZds1 in the rice blast fungus[J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 559-568.

Figures/Tables 8

Fig.1 Identification and analysis of MoZDS1

Fig.2 Domain structure analysis of MoZds_C in M. oryzae

Fig.3 Phenotypic analysis of ΔMozds1 mutants Asterisks represent significant differences (*, P<0.05; **, P<0.01), the same as below. Scale =10 μm.

Fig.4 Pathogenicity analysis of ΔMozds1 mutants

Fig.5 Influence of stress environment on MoZds1

Fig.6 Fluorescence localization analysis of GFP-MoZds1 Fluorescence localization of GFP-MoZds1 in conidia and mycelia, scale =10 μm.

Fig.7 Observation of glycogen degradation of conidia and appressorium Scale =10 μm.

Fig.8 GFP-Atg8 and GFP expression levels

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Functional study of MoZds1 in the rice blast fungus

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