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cDNA cloning and sequence analysis of Apo A1, CD36 and DGAT2 genes from goose#br#

  

  1. (1 Jinhua Career Technical College, Jinhua 321000, China; 2 Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3 College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 4 Rongyao Goose Industry Co., Ltd of Changxing, Huzhou 313100, China)
  • Online:2015-10-25 Published:2015-10-20

Abstract: The cDNA sequences of goose Apolipoprotein A\|1 (Apo A1), Fatty acid translocase (CD36) and Diacylglycerol O\|acyltransferase 2 (DGAT2) genes including full-length coding sequences (CDS) were cloned by RACE technology. It was shown that the length of goose Apo A1 cDNA was 1 106 bp (GenBank accession: KF460562), which consisted of 135 bp 5’UTR (untranslated region, UTR) sequence, 795 bp coding sequence (encoding 265 amino acids) and 176 bp 3′UTR sequence. The length of goose CD36 cDNA sequence was 2 262 bp (GenBank accession: KF460563), which consisted of 196 bp 5’UTR sequence, 1 416 bp coding sequence and 650 bp 3' UTR sequence, encoding 472 amino acids. The length of cDNA sequence of goose DGAT2 gene was 1 341 bp (GenBank accession: KF460564), consisted of 93 bp 5 ′UTR sequence, 1 077 bp coding sequence and 171 bp 3′ UTR sequence, encoding 359 amino acids. Homology analysis results indicated that goose and duck showed the highest homology (>90%) on the above three genes, while the homology with genes from human and other mammals were relatively low.

Key words:  Apo A1, CD36, DGAT2, goose