Abstract: The present study was conducted by enzyme\|linked immunosorbent assay （ELISA） to determine aflatoxin B1 （AFB1） content in bottled Chinese rice wine. The linearity， detection sensitivity， recovery and repeatability were verified. High correlation coefficient was obtained （R2 =0.998 2） within the linear range （0.1～2.0 μg·L-1）. The detection limit for AFB1 content was 005 μg·kg-1. The average spiked recovery rate was 90.2%～106.0%， and the repeatability was 3.5%.The developed method was applied to 26 bottled Chinese rice wine collected from local markets. AFB1 was found within all collected samples， but the content of AFB1 in each wine was all below 2.0 μg·kg-1. This method could be widely applied to determine aflatoxin B1 content in a large number of commercially available Chinese rice wine.

Key words: enzyme\, linked immunosorbent assay （ELISA）, aflatoxin B1, Chinese rice wine