Abstract: Rapid large\|scale propagation of submerged vegetation seedlings has been one of the difficulties in submerged vegetation restoration， ecological restoration and the aquatic environment. According to the low cost and efficient open tissue culture technology application， bacteriostatic agent selection was the key technology. Mancozeb and Kathon with different concentrations were added to MS medium to study their antibacterial action in open tissue culture of Potamogeton malaianus. The results showed that Mancozeb and Kathon showed ideal bacteriostatic effects on Potamogeton malaianus with the concentrations of 0.07-0.10·L-1 and 0.1%， respectively， which have good application value.

Key words: Potamogeton malaianus, open tissue culture, Mancozeb, Kathon