Abstract: In order to obtain the optimum extraction conditions of total flavonoids from Chrysanthemum coronarium L. seeds， the factors affecting the extraction rate of total flavonoids were evaluated by single factor experiment firstly and three main factors including the ethanol concentration， the ratio of solvent to sample and the microwave\|assisted extraction temperature were selected. Further， these factors were optimized using quadratic orthogonal rotation combination design. The optimal extraction conditions were determined as follows: the microwave\|assisted extraction temperature 72℃， the ethanol concentration 68%， and the ratio of solvent to sample 25:1. Under the optimal extraction conditions， the predicted extraction rate of total flavonoids was 329%， the measured value was 315%， and the standard deviation was -0042 5. The result indicated that the extraction technology of total flavonoids from Chrysanthemum coronarium L. seeds could be optimized by the quadratic regression orthogonal rotary method.

Key words: Chrysanthemum coronarium L. seeds；total flavonoids； quadratic regression orthogonal rotary； microwave\, assisted extraction