Abstract: In the present study， seroepidemiological investigation on duck Tembusu virus （TMUV） antibody level was carried out. A total of 4 989 sera samples were collected from duck， goose and chicken during 2010-2014 in Zhejiang Province. The TMUV antibody levels of collected samples were detected by ELISA method. It was shown that the positive rates of TMUV antibody in duck， goose and chicken samples were 27.33%， 694% and 111%， respectively. By yearly analysis， TMUV antibody positive rate was 54.83% in the infected duck flocks in 2010-2011 during epidemic period. Goose and chicken sera samples were not collected in that period. TMUV positive rates in the infected duck and goose flock were 3388% and 20.00%， respectively， in 2012， and positive serum sample was not found in chicken flocks. TMUV positive rates in the infected duck， goose and chicken flock were 49.68%， 10.00% and 28.00%， respectively in 2013. TMUV positive rates in the infected duck and goose flock were 39.19% and 6.67%， respectively， in 2014， and no positive serum sample was found in the chicken flocks. In conclusion， the highest TMUV antibody level was found in the year of 2010-2011 during the epidemic period， and duck was the main TMUV infected poultry.

Key words: Tembusu virus, ELISA antibody, seroepidemiological investigation