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Abstract: In order to study polymorphism of SLADRA exon 2 in Bamei pigs, genetic variation of SLADRA exon 2 in Bamei pigs was identified by polymerase chain reactionsingle strand conformation polymorphism and cloning, both the nucleotide and the amino acid sequences of the different alleles were analyzed. The results showed that two alleles (A, B) and three genotypes (AA, BB, AB) of SLADRA exon 2 were detected from 222 Bamei pigs, the frequencies of the A allele and AA genotype were the highest, and A and AA were dominant. The results of sequencing showed that only one mutational nucleotide site (c.3093 A→C) and one amino acid site (p. Met→Leu) were identified. Genetic heterozygosity was 0.351 4, and polymorphic information content (PIC) was 0.308 8, which meaned SLADRA exon 2 in Bamei pigs was moderately polymorphic (0.25 <PIC <0.5). The chisquare analysis suggested that the allele and genotype frequencies were not deviated from HardyWeinberg equilibrium (P > 0.05).The finding showed that polymorphism of SLADRA exon 2 in Bamei pigs was low.
Key words: Bamei pigs, SLADRA, PCRSSCP
JIN Li, TAN Xiaoyin, MU Tong, WANG Guomei, ZHANG Li, LIU Lixia*. Polymorphism of SLADRA exon 2 in Bamei pigs[J]. .
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http://www.zjnyxb.cn/EN/Y2016/V28/I6/922