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Polymorphism of SLADRA exon 2 in Bamei pigs

  

  1. (College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China)
  • Online:2016-06-25 Published:2016-06-12

Abstract: In order to study polymorphism of SLADRA exon 2 in Bamei pigs, genetic variation of SLADRA exon 2 in Bamei pigs was identified by polymerase chain reactionsingle strand conformation polymorphism and cloning, both the nucleotide and the amino acid sequences of the different alleles were analyzed. The results showed that two alleles (A, B) and three genotypes (AA, BB, AB) of SLADRA exon 2 were detected from 222 Bamei pigs, the frequencies of the A allele and AA genotype were the highest, and A and AA were dominant. The results of sequencing showed that only one mutational nucleotide site (c.3093 A→C) and one amino acid site (p. Met→Leu) were identified. Genetic heterozygosity was 0.351 4, and polymorphic information content (PIC) was 0.308 8, which meaned SLADRA exon 2 in Bamei pigs was moderately polymorphic (0.25 <PIC <0.5). The chisquare analysis suggested that the allele and genotype frequencies were not deviated from HardyWeinberg equilibrium (P > 0.05).The finding showed that polymorphism of SLADRA exon 2 in Bamei pigs was low.

Key words: Bamei pigs, SLADRA, PCRSSCP