›› 2017, Vol. 29 ›› Issue (6): 994-1000.DOI: 10.3969/j.issn.1004-1524.2017.06.20

• Food Science • Previous Articles     Next Articles

Rapid detection of chicken,duck and pork blending in beef products by multiple digital PCR

YANG Hua, WANG Xiaofu, XIAO Yingping, WEI Wei, XU Junfeng   

  1. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2017-04-24 Online:2017-06-20 Published:2017-09-07

Abstract: In this study,a quickly detective method,using droplet digital PCR,for detection of beef,chicken,duck and pork was established. The results showed that the selected primers and probes for the four species had good specificity,and could quickly make out these four kinds of mean component in tested samples. In all of the digital PCR analysis, the fluorescence signals of FAM and HEX can be well distinguished. For detection of 9 samples,the numbers of droplets generated in the multiple digital PCR were all above 10 000, which could meet the requirements for the poisson formula analysis in digital PCR. meanwhile,the test results of the 9 samples using multiple digital were consistent with the known information of these samples.

Key words: digital PCR, meat components detection, multiple detection, quickly detection

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