Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene

doi:10.3969/j.issn.1004-1524.2017.08.07

›› 2017, Vol. 29 ›› Issue (8): 1271-1280.DOI: 10.3969/j.issn.1004-1524.2017.08.07

• Animal Science • Previous Articles Next Articles

Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene

ZHOU Xiumin, YANG Yongjiang, BI Yingjie, REN Weihe, ZHANG Li^*

College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China

Received:2017-05-14 Online:2017-08-20 Published:2017-09-06

Abstract

Abstract: Based on the mitochondrial DNA COX Ⅰ (mtDNA COX Ⅰ) gene, the genetic diversity and phylogenetic relationships of 6 pig breeds (Large White pig, Landrace pig, Duroc pig, Zang pig, Gansu black pig and Bamei pig) were studied. The mtDNA COX Ⅰ gene sequences obtained from 6 pig breeds were sequenced and analyzed. Besides, we constructed phylogenetic tree by using MEGA 6.0 analysis software and neighbor joining method based on Kimurk two parameter models. The results showed that there were 21 mutations in sequences of COX Ⅰ (1 545 bp), including 8 group specific mutations. Landrace pig and Duroc pig were abundant in polymorphism (13 same mutation sites), and the ratios (R) of nucleotides’ conversion number (si) and transition number (sv) were 12 and 15, which proved sequence substitutions were far from saturation. While the polymorphisms of Zang pigs, Gansu black pigs and Bamei pigs were poor. The introduced pigs were used as sire, not dam, to improve the growth performance and lean meat percentage of the domestic pig breeds. The mtDNA COX Ⅰ gene can effectively distinguish the relationship between six pig breeds. This study can provide theoretical basis for the effective protection and rational utilization of pig breeds in our country.

Key words: pig, mitochondrial cytochrome C oxidase subunit Ⅰ, phylogeography

CLC Number:

S828

ZHOU Xiumin, YANG Yongjiang, BI Yingjie, REN Weihe, ZHANG Li. Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene[J]. , 2017, 29(8): 1271-1280.

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Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene

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