Optimization of extraction process of total flavonoids from Chimonanthus salicifolius S.Y.H by HPLC combined with response surface methodology and its antibacterial activity

doi:10.3969/j.issn.1004-1524.2018.02.17

›› 2018, Vol. 30 ›› Issue (2): 298-306.DOI: 10.3969/j.issn.1004-1524.2018.02.17

• Food Science • Previous Articles Next Articles

Optimization of extraction process of total flavonoids from Chimonanthus salicifolius S.Y.H by HPLC combined with response surface methodology and its antibacterial activity

WEN Huiping¹, XIAO Jianzhong^{1, *}, LEI Weimin¹, JI Jiana²

1. College of Ecology, Lishui University, Lishui 323000, China;
2. Technical Service Center, Lishui Entry-Exit Inspcction and Quarantine, Lishui 323000, China

Received:2017-11-20 Online:2018-02-20 Published:2018-02-11

Abstract

Abstract: The contents of rutin, quercetin and kaempferol extracted from Chimonanthus salicifolius were determined by high performance liquid chromatography (HPLC). Based on the single factor analysis, the effect of ethanol concentration, extraction time and extraction temperature on the yield of flavonoids from C. salicifolius were studied by response surface methodology. Meanwhile, the antibacterial activity of flavonoids from C. salicifolius was assessed by filter paper method. The results showed that main factors affecting the extraction of total flavonoids from C. salicifolius were ethanol concentration, extraction time, extraction temperature. The optimum extraction conditions of total flavonoids were: extraction temperature of 90 ℃, solid-liquid ratio of 1:15, ethanol concentration of 79%, extraction time of 1.7 h. Under the optimal conditions, contents of rutin, quercetin, kaempferol were 0.861, 0.504, 0.492 mg·g^-1, respectively; and the extraction yield of total flavonoids was 1.857 mg·g^-1 which differed from the theoretical value of 1.50%,the total flavonoids extracted from Chimonanthus salicifolius had antibacterial activity against many kinds of bacteria, especially Bacillus cereus and Escherichia coli, the mean diameters of the inhibition zones about them were 13.21 and 11.18 mm, respectively. Therefore, response surface methodology was feasible for the extraction optimization of total flavonoids from C. salicifolius. The total flavonoids from C. salicifolius had good antibacterial activity, indicating that it might have great application prospects in the fields of medicine.

Key words: Chimonanthus salicifolius, flavonoids, response surface methodology, antibacterial activity

CLC Number:

TQ461

WEN Huiping, XIAO Jianzhong, LEI Weimin, JI Jiana. Optimization of extraction process of total flavonoids from Chimonanthus salicifolius S.Y.H by HPLC combined with response surface methodology and its antibacterial activity[J]. , 2018, 30(2): 298-306.

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Optimization of extraction process of total flavonoids from Chimonanthus salicifolius S.Y.H by HPLC combined with response surface methodology and its antibacterial activity

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